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The map label for this gene is ruvC

Identifier: 15618531

GI number: 15618531

Start: 714141

End: 714647

Strand: Reverse

Name: ruvC

Synonym: CPn0621

Alternate gene names: 15618531

Gene position: 714647-714141 (Counterclockwise)

Preceding gene: 15618532

Following gene: 15618530

Centisome position: 58.09

GC content: 42.41

Gene sequence:

>507_bases
GTGTCAGAACTGATTATAGGTGTGGATCCAGGAACGATAGTCGCAGGATATGCCATCATTGCAGTGGAACAGCGCTATCA
ATTACGTCCTTATAGTTATGGTGCCATTCGTTTATCTTCCGACATGCCACTGCCTATGCGCTATAAGACTTTGTTTGAGC
AACTCTCAGGAGTATTAGATGATACGCAACCTAATGCTATGGTCCTAGAAACGCAATTTGTAAATAAGAATCCTCAAAGT
ACTATGAAATTAGCTATGGCACGAGGAATCGTTTTATTGGCTGCAGCTCAGCGTGATATCCTCATTTTTGAATATGCCCC
AAATGTTGCTAAGAAAGCAGTAGTCGGTAAGGGTCATGCAAGTAAAAGACAGGTCCAGGTGATGGTAAGCAAGATTCTAA
ATGTTCCCGAAGTTTTGCACCCTTCGAATGAAGATATTGCTGATGCCTTTGCACTTGCTATATGTCATACTCATGTAGCG
CGTAGCCCTCTTTGTGGAGTGAGATAA

Upstream 100 bases:

>100_bases
TTTTTTTCTAGAAACCGAGAATTCACTACTTGAGAAGTGATTATCTAGGTTAGGATCATCTTTTAAAGACAATAAGTTCA
AAGTATTTCAGGAATCGATT

Downstream 100 bases:

>100_bases
GATGTACGACTATATTCGTGGAACACTGACCTATGTGCATACTGGTGCAATTGTTATAGAATGCCAAGGTATTGGCTACC
ATATTGCTATTACAGAACGT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSELIIGVDPGTIVAGYAIIAVEQRYQLRPYSYGAIRLSSDMPLPMRYKTLFEQLSGVLDDTQPNAMVLETQFVNKNPQS
TMKLAMARGIVLLAAAQRDILIFEYAPNVAKKAVVGKGHASKRQVQVMVSKILNVPEVLHPSNEDIADAFALAICHTHVA
RSPLCGVR

Sequences:

>Translated_168_residues
MSELIIGVDPGTIVAGYAIIAVEQRYQLRPYSYGAIRLSSDMPLPMRYKTLFEQLSGVLDDTQPNAMVLETQFVNKNPQS
TMKLAMARGIVLLAAAQRDILIFEYAPNVAKKAVVGKGHASKRQVQVMVSKILNVPEVLHPSNEDIADAFALAICHTHVA
RSPLCGVR
>Mature_167_residues
SELIIGVDPGTIVAGYAIIAVEQRYQLRPYSYGAIRLSSDMPLPMRYKTLFEQLSGVLDDTQPNAMVLETQFVNKNPQST
MKLAMARGIVLLAAAQRDILIFEYAPNVAKKAVVGKGHASKRQVQVMVSKILNVPEVLHPSNEDIADAFALAICHTHVAR
SPLCGVR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=133, Percent_Identity=39.0977443609023, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CHLPN (Q9Z7T3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72057
- PIR:   B86568
- RefSeq:   NP_224817.1
- RefSeq:   NP_300677.1
- RefSeq:   NP_444678.1
- RefSeq:   NP_876919.1
- ProteinModelPortal:   Q9Z7T3
- SMR:   Q9Z7T3
- GeneID:   1467326
- GeneID:   895409
- GeneID:   919385
- GeneID:   963017
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0126
- KEGG:   cpn:CPn0621
- KEGG:   cpt:CpB0647
- TIGR:   CP_0126
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- PhylomeDB:   Q9Z7T3
- ProtClustDB:   PRK00039
- BioCyc:   CPNE115711:CP_0126-MONOMER
- BioCyc:   CPNE115713:CPN0621-MONOMER
- BioCyc:   CPNE138677:CPJ0621-MONOMER
- BioCyc:   CPNE182082:CPB0647-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18404; Mature: 18273

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIIGVDPGTIVAGYAIIAVEQRYQLRPYSYGAIRLSSDMPLPMRYKTLFEQLSGVLD
CCCEEEECCCCHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
DTQPNAMVLETQFVNKNPQSTMKLAMARGIVLLAAAQRDILIFEYAPNVAKKAVVGKGHA
CCCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHCCCCC
SKRQVQVMVSKILNVPEVLHPSNEDIADAFALAICHTHVARSPLCGVR
HHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SELIIGVDPGTIVAGYAIIAVEQRYQLRPYSYGAIRLSSDMPLPMRYKTLFEQLSGVLD
CCEEEECCCCHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
DTQPNAMVLETQFVNKNPQSTMKLAMARGIVLLAAAQRDILIFEYAPNVAKKAVVGKGHA
CCCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHCCCCC
SKRQVQVMVSKILNVPEVLHPSNEDIADAFALAICHTHVARSPLCGVR
HHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362