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The map label for this gene is sucB_2

Identifier: 15618438

GI number: 15618438

Start: 608723

End: 609904

Strand: Reverse

Name: sucB_2

Synonym: CPn0527

Alternate gene names: 15618438

Gene position: 609904-608723 (Counterclockwise)

Preceding gene: 15618439

Following gene: 15618437

Centisome position: 49.58

GC content: 43.99

Gene sequence:

>1182_bases
ATGATATTTGAGTTCCGATTCCCTAAAATAGGAGAGACGAGTTCCGGAGGATCTATAGTCCGTTGGTTAAAAAATTTGGG
TGATCATGTAGCTAGAGATGAGCCTCTGATTGAAGTATCTACGGATAAAATTGCTACAGAATTACCCTCTCCTAAAGCAG
GCCGACTGGTGCGTTTCTGCGTCAATGAGGGAGACGAGGTTGCTTCTGGGGATGTTTTAGGATTGATAGAGCTTGAGGAG
ATTTCCGAAGCTGATGATGAGAGCACCTCATGTCCTCCGACTTCTTGTGAAACAAAGTCGGAGGCGGGTTCCAGCAGTTC
TTCGGTATGGTTTTCTCCTGCCGTGCTGAGTTTAGCTCAACGTGAAGGCATTGGTCTTGATAACCTCCAAAAGATTGCCG
GCACGGGGAAAGGGGGACGAGTGACTCGTCAGGATTTAGAAGCGTATATTTCAGAATCGCAACAAGTTTCTATTCCCGAA
ATATTTCAAGGAGAAGTGAATCGCATTCCTATGTCTCCGCTACGTCGGGCAATAGCTTCTTCTCTCTCCAAGTCTTCAGA
TGAGGTTCCTCACGCATCTTTGGTTGTTGATGTCGATGTCACAGATCTTATGAATCTGATTTCTGGTGAACGCCAACGCT
TCTTAGATACGCATGGGGTGAAGCTAACGATTACAAGTTTCATTGTACAGTGTTTAGCTCAGACTTTAAGGCAGTTTCCT
TTATTGAATGGTTCCTTAGATGGGACTACCATTGTTATGAAGAAATCTGTGAATGTAGGCGTTGCCGTGAACCTCAATAA
GGAAGGGGTTGTTGTTCCTGTCATCCACAATTGTCAAGATCGCGGTTTAGTAAGTATTGCAAAGGCCTTGGCGGATCTAT
CTTCAAGGGCTCGGTTAAATAAATTGGATCCTAGTGAAGTGCAAGATGGCAGCGTTACTGTCACGAATTTTGGAATGACG
GGAGCTTTGATTGGGATGCCCATCATACGTTATCCTGAAGTTGCTATTTTAGGAATTGGCACAATACAAAAACGTGTTGT
CGTCCGTGATGACGATTCTTTAGCCATTCGCAAAATGGTCTATGTGACACTTACCTTTGACCATAGAGTATTGGATGGTA
TTTACGGCAGTGAGTTTTTAACCTCATTGAAAAATCGTTTGGAGTCTGTTACGATGGGCTAA

Upstream 100 bases:

>100_bases
GCTGTAGTTGCTACTTATGTAGCTTCTGGAGAAGGAGAGCTTTCTCCTTATGAATCGATAAAACAGGAAAGCGTTGAAAC
TACATAGAAGGTAACGATAC

Downstream 100 bases:

>100_bases
AGTGTAGCCAATCCAAAGAGAACCGAATGCCTTCCCCGATGATTTCTACTGACGTATGCCAAGACATTCTAGGTAAGCAA
AAAGAAGCTGTAGATTTTTT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MIFEFRFPKIGETSSGGSIVRWLKNLGDHVARDEPLIEVSTDKIATELPSPKAGRLVRFCVNEGDEVASGDVLGLIELEE
ISEADDESTSCPPTSCETKSEAGSSSSSVWFSPAVLSLAQREGIGLDNLQKIAGTGKGGRVTRQDLEAYISESQQVSIPE
IFQGEVNRIPMSPLRRAIASSLSKSSDEVPHASLVVDVDVTDLMNLISGERQRFLDTHGVKLTITSFIVQCLAQTLRQFP
LLNGSLDGTTIVMKKSVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARLNKLDPSEVQDGSVTVTNFGMT
GALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMG

Sequences:

>Translated_393_residues
MIFEFRFPKIGETSSGGSIVRWLKNLGDHVARDEPLIEVSTDKIATELPSPKAGRLVRFCVNEGDEVASGDVLGLIELEE
ISEADDESTSCPPTSCETKSEAGSSSSSVWFSPAVLSLAQREGIGLDNLQKIAGTGKGGRVTRQDLEAYISESQQVSIPE
IFQGEVNRIPMSPLRRAIASSLSKSSDEVPHASLVVDVDVTDLMNLISGERQRFLDTHGVKLTITSFIVQCLAQTLRQFP
LLNGSLDGTTIVMKKSVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARLNKLDPSEVQDGSVTVTNFGMT
GALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMG
>Mature_393_residues
MIFEFRFPKIGETSSGGSIVRWLKNLGDHVARDEPLIEVSTDKIATELPSPKAGRLVRFCVNEGDEVASGDVLGLIELEE
ISEADDESTSCPPTSCETKSEAGSSSSSVWFSPAVLSLAQREGIGLDNLQKIAGTGKGGRVTRQDLEAYISESQQVSIPE
IFQGEVNRIPMSPLRRAIASSLSKSSDEVPHASLVVDVDVTDLMNLISGERQRFLDTHGVKLTITSFIVQCLAQTLRQFP
LLNGSLDGTTIVMKKSVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARLNKLDPSEVQDGSVTVTNFGMT
GALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMVYVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=429, Percent_Identity=27.7389277389277, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI110671329, Length=416, Percent_Identity=25.4807692307692, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI203098753, Length=445, Percent_Identity=27.8651685393258, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI203098816, Length=445, Percent_Identity=27.8651685393258, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=31.6964285714286, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786946, Length=399, Percent_Identity=29.0726817042606, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=30.2211302211302, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=394, Percent_Identity=30.2030456852792, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=423, Percent_Identity=27.6595744680851, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=25.4196642685851, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=33.1818181818182, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=383, Percent_Identity=26.6318537859008, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.2331838565022, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859875, Length=422, Percent_Identity=26.7772511848341, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=32.7354260089686, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=32.7354260089686, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 42486; Mature: 42486

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFEFRFPKIGETSSGGSIVRWLKNLGDHVARDEPLIEVSTDKIATELPSPKAGRLVRFC
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCHHEE
VNEGDEVASGDVLGLIELEEISEADDESTSCPPTSCETKSEAGSSSSSVWFSPAVLSLAQ
CCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHH
REGIGLDNLQKIAGTGKGGRVTRQDLEAYISESQQVSIPEIFQGEVNRIPMSPLRRAIAS
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHH
SLSKSSDEVPHASLVVDVDVTDLMNLISGERQRFLDTHGVKLTITSFIVQCLAQTLRQFP
HHHCCCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCC
LLNGSLDGTTIVMKKSVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARLN
CCCCCCCCEEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
KLDPSEVQDGSVTVTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMV
CCCCCCCCCCCEEEEECCCHHHHHCCCHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEE
YVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMG
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFEFRFPKIGETSSGGSIVRWLKNLGDHVARDEPLIEVSTDKIATELPSPKAGRLVRFC
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCCHHEE
VNEGDEVASGDVLGLIELEEISEADDESTSCPPTSCETKSEAGSSSSSVWFSPAVLSLAQ
CCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHH
REGIGLDNLQKIAGTGKGGRVTRQDLEAYISESQQVSIPEIFQGEVNRIPMSPLRRAIAS
HCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHH
SLSKSSDEVPHASLVVDVDVTDLMNLISGERQRFLDTHGVKLTITSFIVQCLAQTLRQFP
HHHCCCCCCCCEEEEEECCHHHHHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCC
LLNGSLDGTTIVMKKSVNVGVAVNLNKEGVVVPVIHNCQDRGLVSIAKALADLSSRARLN
CCCCCCCCEEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
KLDPSEVQDGSVTVTNFGMTGALIGMPIIRYPEVAILGIGTIQKRVVVRDDDSLAIRKMV
CCCCCCCCCCCEEEEECCCHHHHHCCCHHCCCCEEEEECCCCCEEEEEECCCCCEEEEEE
YVTLTFDHRVLDGIYGSEFLTSLKNRLESVTMG
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]