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The map label for this gene is artJ

Identifier: 15618393

GI number: 15618393

Start: 560958

End: 561737

Strand: Reverse

Name: artJ

Synonym: CPn0482

Alternate gene names: 15618393

Gene position: 561737-560958 (Counterclockwise)

Preceding gene: 15618397

Following gene: 15618392

Centisome position: 45.66

GC content: 41.03

Gene sequence:

>780_bases
ATGATAAAACAAATAGGCCGTTTTTTTAGAGCATTTATTTTTATAATGCCTTTATCTTTAACAAGTTGTGAGTCTAAAAT
CGATCGAAATCGCATCTGGATTGTAGGTACGAATGCTACATATCCTCCTTTTGAGTATGTGGATGCTCAGGGGGAAGTTG
TAGGTTTCGATATAGATTTGGCAAAGGCAATTAGTGAAAAACTTGGCAAGCAATTGGAAGTTAGAGAATTCGCTTTCGAT
GCTTTAATTTTAAATTTAAAAAAACATCGTATCGATGCAATTTTAGCAGGAATGTCCATTACTCCTTCGCGTCAGAAGGA
AATCGCCCTGCTTCCCTATTATGGCGATGAGGTTCAAGAGCTGATGGTGGTTTCTAAGCGGTCTTTAGAGACCCCTGTGC
TTCCCCTAACACAGTATTCTTCTGTTGCTGTTCAGACAGGAACGTTTCAGGAGCATTATCTTTTATCTCAGCCCGGAATT
TGTGTCCGTTCTTTTGATAGCACCTTGGAGGTGATTATGGAAGTTCGTTATGGGAAATCTCCGGTTGCCGTTCTAGAACC
CTCGGTAGGACGTGTCGTTCTTAAAGACTTCCCTAATCTTGTTGCAACAAGATTAGAGCTCCCTCCTGAATGTTGGGTGT
TGGGCTGTGGTCTCGGCGTAGCTAAAGATCGTCCTGAAGAAATACAAACGATTCAACAAGCGATTACAGATTTAAAGAGC
GAAGGGGTGATTCAATCTTTAACCAAGAAATGGCAACTTTCTGAAGTTGCTTACGAATAG

Upstream 100 bases:

>100_bases
AAAATTATTCAAAGTTAAAACTTTTTCGAGTTTGATGTATTTATTTTTTTATGTAAACTTTAACACATGATAGAATTTAG
CGTATAGAGCGCAAACTTTC

Downstream 100 bases:

>100_bases
AGGGTATTCTTATGGCAACCTCTGTTCCTGTAACTTCATCTACTTCTGTAGGAGAGGCTAACTCCTCCAACGAAAGATTT
ACTGAACGAACATCGCGAAT

Product: arginine periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIKQIGRFFRAFIFIMPLSLTSCESKIDRNRIWIVGTNATYPPFEYVDAQGEVVGFDIDLAKAISEKLGKQLEVREFAFD
ALILNLKKHRIDAILAGMSITPSRQKEIALLPYYGDEVQELMVVSKRSLETPVLPLTQYSSVAVQTGTFQEHYLLSQPGI
CVRSFDSTLEVIMEVRYGKSPVAVLEPSVGRVVLKDFPNLVATRLELPPECWVLGCGLGVAKDRPEEIQTIQQAITDLKS
EGVIQSLTKKWQLSEVAYE

Sequences:

>Translated_259_residues
MIKQIGRFFRAFIFIMPLSLTSCESKIDRNRIWIVGTNATYPPFEYVDAQGEVVGFDIDLAKAISEKLGKQLEVREFAFD
ALILNLKKHRIDAILAGMSITPSRQKEIALLPYYGDEVQELMVVSKRSLETPVLPLTQYSSVAVQTGTFQEHYLLSQPGI
CVRSFDSTLEVIMEVRYGKSPVAVLEPSVGRVVLKDFPNLVATRLELPPECWVLGCGLGVAKDRPEEIQTIQQAITDLKS
EGVIQSLTKKWQLSEVAYE
>Mature_259_residues
MIKQIGRFFRAFIFIMPLSLTSCESKIDRNRIWIVGTNATYPPFEYVDAQGEVVGFDIDLAKAISEKLGKQLEVREFAFD
ALILNLKKHRIDAILAGMSITPSRQKEIALLPYYGDEVQELMVVSKRSLETPVLPLTQYSSVAVQTGTFQEHYLLSQPGI
CVRSFDSTLEVIMEVRYGKSPVAVLEPSVGRVVLKDFPNLVATRLELPPECWVLGCGLGVAKDRPEEIQTIQQAITDLKS
EGVIQSLTKKWQLSEVAYE

Specific function: Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Secreted. Cell surface

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1787085, Length=219, Percent_Identity=31.5068493150685, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787088, Length=219, Percent_Identity=31.9634703196347, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=31.8965517241379, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788649, Length=228, Percent_Identity=30.2631578947368, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788228, Length=266, Percent_Identity=23.3082706766917, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ARTJ_CHLPN (Q9Z869)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   F72073
- PIR:   F86550
- PIR:   G81595
- RefSeq:   NP_224678.1
- RefSeq:   NP_300537.1
- RefSeq:   NP_444823.1
- RefSeq:   NP_876774.1
- PDB:   3N26
- PDBsum:   3N26
- ProteinModelPortal:   Q9Z869
- GeneID:   1467181
- GeneID:   895494
- GeneID:   919218
- GeneID:   962901
- GenomeReviews:   AE001363_GR
- KEGG:   cpn:CPn0482
- TIGR:   CP_0272
- HOGENOM:   HBG698334
- ProtClustDB:   CLSK871344
- BioCyc:   CPNE115713:CPN0482-MONOMER
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 29070; Mature: 29070

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 38-38 BINDING 104-104 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQIGRFFRAFIFIMPLSLTSCESKIDRNRIWIVGTNATYPPFEYVDAQGEVVGFDIDL
CHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCEEEECHHH
AKAISEKLGKQLEVREFAFDALILNLKKHRIDAILAGMSITPSRQKEIALLPYYGDEVQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHH
LMVVSKRSLETPVLPLTQYSSVAVQTGTFQEHYLLSQPGICVRSFDSTLEVIMEVRYGKS
HHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHEEECCCCCEEHHHHHHHHHHHHHHCCCC
PVAVLEPSVGRVVLKDFPNLVATRLELPPECWVLGCGLGVAKDRPEEIQTIQQAITDLKS
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
EGVIQSLTKKWQLSEVAYE
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKQIGRFFRAFIFIMPLSLTSCESKIDRNRIWIVGTNATYPPFEYVDAQGEVVGFDIDL
CHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCEEEECHHH
AKAISEKLGKQLEVREFAFDALILNLKKHRIDAILAGMSITPSRQKEIALLPYYGDEVQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHH
LMVVSKRSLETPVLPLTQYSSVAVQTGTFQEHYLLSQPGICVRSFDSTLEVIMEVRYGKS
HHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHEEECCCCCEEHHHHHHHHHHHHHHCCCC
PVAVLEPSVGRVVLKDFPNLVATRLELPPECWVLGCGLGVAKDRPEEIQTIQQAITDLKS
CCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHC
EGVIQSLTKKWQLSEVAYE
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362