Click here to switch to the map view.

The map label for this gene is lepB

Identifier: 15618034

GI number: 15618034

Start: 141824

End: 143734

Strand: Reverse

Name: lepB

Synonym: CPn0110

Alternate gene names: 15618034

Gene position: 143734-141824 (Counterclockwise)

Preceding gene: 15618035

Following gene: 15618032

Centisome position: 11.68

GC content: 40.55

Gene sequence:

>1911_bases
ATGAAACAACACTATTCTCTAAATAAAAGTCGTCATATCCTCCGCAGTACTTATAAGCTTTTAAAAAGTAAAAAACTCGC
CCATTCCCCTGCAGATAAAAAGCAACTGCAAGAACTACTAGAACAACTAGAAGAGGCTATCTTTGAACATGATCAAGAAA
CTGCAAGCGACTTAGCTCAGCAAGCATTAGCATTTTCCAACCGTTATCCTAATTCCTTCGGACGCAAAACCTATGAGCTT
ATCAAGGCCCTTCTTTTTGCTGGTGTTGTAGCCTTCTTAGTTCGGCAATTTTGGTTTGAACTTTATGAAGTGCCTACAGG
ATCCATGAGGCCTACAATTTTAGAACAGGATCGGATTCTTGTATCCAAAACAACATTTGGTCTCCATTGCCCTTTTGCTA
AGAAACCACTTGCCTTCAATCCTGAATCCGTAACTCGCGGGGGTCTTGTTGTTTTCACTGTAGGCGACCTCCCTATCCCA
GATGCTGATACAAAGTACTTCGGATTGATTCCAGGAAAAAAGCGTTACATTAAACGTTGCATGGGAAGACCTGGGGACTT
CTTATATTTCTATGGAGGAAAAATTTATGGTCTTGATGATGCAGGTAAACGCATAGAGTTTCCTTCTGTCCATGGTTTAG
AAAACTTATATCACGTCCCCTATATATCCTTTGATGGCACTACCAGCAGCCATACAGAAGGGCAGAAAACAATTATAGAT
TTTAAGCAGTTCAATCAAAGTTATGGTCGGCTGATTTTCCCTCAAACCTCCATGTATGGACAATTCTTTGACCATAAAGA
ATGGCATCAAGACGAGCCTAATAAATTAAAAGATCCTCATCTTTCGCCAGTCAGCTATGCCGATCTTTTTGGTATGGGTA
ACTATGCTATGGTGCGCATCTTAACAGAACATCAGGCACGAACATCCCATCTACTTCCGAATCCAGGAAGTCCAACTAAA
GTCTACTTAGAAATTTGCCATACAGCGAACCTTTCCTACCCAAAGCCTCTGTTGCGTCACTATGAGCATCAGCTCTCGCC
TGCGATTCAACCTATGAAGACTTTACTTCCTTTGCGTAAGGAACATTTGCACTTAATTCGGAACAATCTTACTACCTCTC
GTTTTATTGTTGCTCAAGGATGTGCGTATAAATACCATCAATTCAAGATTAACACTTCAGGAATTGCCAAAGCCTATGCA
ATTCTCCTGCCCAAGGTCCCTGATGGTTGTTATGAATATTCTAAAGGCGAAGCGTATCAAATTGGCTTTGGAGAGATTCG
TTATAAGCTAAAATCTTCTCACCCCCTTACTCAGCTCAATGATAAGCAAGTGATTGAACTTTTTAACTGCGGGATCAACT
TTAGTTCTATTTATAATCCTGTGAATCCGCTGCAAGCACCTTTACCTAACCGTTATGCATTCTTTAACCAAGGGAATCTT
TATATCATGGATTCTCCTGTATTTATAAAGAATGATCCAACTCTGCAAAAATTTGTGACTTCTGAAACGGAAAAGCAAGA
GGGGTCTTCAGAGACACAACCCTATATAGCTTTTGTTGACAAGGGACTCCCTCCAGAAGATTTTAAAGAATTCGTGGAGT
TTATACATAATTTTGGTATTCAAGTTCCTAAAGGTCATGTTCTCGTCTTGGGAGATAACTACCCTATGAGTGCGGATAGT
CGAGAATTTGGCTTTGTTCCTATGGAAAATCTCTTAGGATCTCCTCTATGTACATTCTGGCCTATTGGACGCATGGGACG
GTTAACTGGAGTTTCTGCTCCAACAACACTCTCAGGTTATCTTGTTAGTGGGATAGCATTAGCGACGGGTCTCTCTCTCA
TTGGATATGTCTACTATCAAAAACGACGCAGACTCTTTCCTAAGAAAGAGGAGAAAAACCACAAGAAATAA

Upstream 100 bases:

>100_bases
ATTTAGGTCTTCAGTCTCCATTAGCTCTAAAGTATCCAATGCAGCCGTTCTATAATCAATCTAAAGAGATAGAATCTGAC
TTTCTTACCCCAGTATCTTT

Downstream 100 bases:

>100_bases
CCATTCTTATGGTTTTTCAGTTGTTTCTAGAAAATCTCTAAGAATCTATAGAACTAACTGTAATTTCAATTTGCGTTGCA
TGTCCATTAATATCCCAGTT

Product: signal peptidase I

Products: NA

Alternate protein names: Signal Peptidase

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MKQHYSLNKSRHILRSTYKLLKSKKLAHSPADKKQLQELLEQLEEAIFEHDQETASDLAQQALAFSNRYPNSFGRKTYEL
IKALLFAGVVAFLVRQFWFELYEVPTGSMRPTILEQDRILVSKTTFGLHCPFAKKPLAFNPESVTRGGLVVFTVGDLPIP
DADTKYFGLIPGKKRYIKRCMGRPGDFLYFYGGKIYGLDDAGKRIEFPSVHGLENLYHVPYISFDGTTSSHTEGQKTIID
FKQFNQSYGRLIFPQTSMYGQFFDHKEWHQDEPNKLKDPHLSPVSYADLFGMGNYAMVRILTEHQARTSHLLPNPGSPTK
VYLEICHTANLSYPKPLLRHYEHQLSPAIQPMKTLLPLRKEHLHLIRNNLTTSRFIVAQGCAYKYHQFKINTSGIAKAYA
ILLPKVPDGCYEYSKGEAYQIGFGEIRYKLKSSHPLTQLNDKQVIELFNCGINFSSIYNPVNPLQAPLPNRYAFFNQGNL
YIMDSPVFIKNDPTLQKFVTSETEKQEGSSETQPYIAFVDKGLPPEDFKEFVEFIHNFGIQVPKGHVLVLGDNYPMSADS
REFGFVPMENLLGSPLCTFWPIGRMGRLTGVSAPTTLSGYLVSGIALATGLSLIGYVYYQKRRRLFPKKEEKNHKK

Sequences:

>Translated_636_residues
MKQHYSLNKSRHILRSTYKLLKSKKLAHSPADKKQLQELLEQLEEAIFEHDQETASDLAQQALAFSNRYPNSFGRKTYEL
IKALLFAGVVAFLVRQFWFELYEVPTGSMRPTILEQDRILVSKTTFGLHCPFAKKPLAFNPESVTRGGLVVFTVGDLPIP
DADTKYFGLIPGKKRYIKRCMGRPGDFLYFYGGKIYGLDDAGKRIEFPSVHGLENLYHVPYISFDGTTSSHTEGQKTIID
FKQFNQSYGRLIFPQTSMYGQFFDHKEWHQDEPNKLKDPHLSPVSYADLFGMGNYAMVRILTEHQARTSHLLPNPGSPTK
VYLEICHTANLSYPKPLLRHYEHQLSPAIQPMKTLLPLRKEHLHLIRNNLTTSRFIVAQGCAYKYHQFKINTSGIAKAYA
ILLPKVPDGCYEYSKGEAYQIGFGEIRYKLKSSHPLTQLNDKQVIELFNCGINFSSIYNPVNPLQAPLPNRYAFFNQGNL
YIMDSPVFIKNDPTLQKFVTSETEKQEGSSETQPYIAFVDKGLPPEDFKEFVEFIHNFGIQVPKGHVLVLGDNYPMSADS
REFGFVPMENLLGSPLCTFWPIGRMGRLTGVSAPTTLSGYLVSGIALATGLSLIGYVYYQKRRRLFPKKEEKNHKK
>Mature_636_residues
MKQHYSLNKSRHILRSTYKLLKSKKLAHSPADKKQLQELLEQLEEAIFEHDQETASDLAQQALAFSNRYPNSFGRKTYEL
IKALLFAGVVAFLVRQFWFELYEVPTGSMRPTILEQDRILVSKTTFGLHCPFAKKPLAFNPESVTRGGLVVFTVGDLPIP
DADTKYFGLIPGKKRYIKRCMGRPGDFLYFYGGKIYGLDDAGKRIEFPSVHGLENLYHVPYISFDGTTSSHTEGQKTIID
FKQFNQSYGRLIFPQTSMYGQFFDHKEWHQDEPNKLKDPHLSPVSYADLFGMGNYAMVRILTEHQARTSHLLPNPGSPTK
VYLEICHTANLSYPKPLLRHYEHQLSPAIQPMKTLLPLRKEHLHLIRNNLTTSRFIVAQGCAYKYHQFKINTSGIAKAYA
ILLPKVPDGCYEYSKGEAYQIGFGEIRYKLKSSHPLTQLNDKQVIELFNCGINFSSIYNPVNPLQAPLPNRYAFFNQGNL
YIMDSPVFIKNDPTLQKFVTSETEKQEGSSETQPYIAFVDKGLPPEDFKEFVEFIHNFGIQVPKGHVLVLGDNYPMSADS
REFGFVPMENLLGSPLCTFWPIGRMGRLTGVSAPTTLSGYLVSGIALATGLSLIGYVYYQKRRRLFPKKEEKNHKK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 72508; Mature: 72508

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHYSLNKSRHILRSTYKLLKSKKLAHSPADKKQLQELLEQLEEAIFEHDQETASDLAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QALAFSNRYPNSFGRKTYELIKALLFAGVVAFLVRQFWFELYEVPTGSMRPTILEQDRIL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEE
VSKTTFGLHCPFAKKPLAFNPESVTRGGLVVFTVGDLPIPDADTKYFGLIPGKKRYIKRC
EEECCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHHHHH
MGRPGDFLYFYGGKIYGLDDAGKRIEFPSVHGLENLYHVPYISFDGTTSSHTEGQKTIID
CCCCCCEEEEECCEEEECCCCCCEECCCCCCCHHHHEECCEEEECCCCCCCCCCCHHHHH
FKQFNQSYGRLIFPQTSMYGQFFDHKEWHQDEPNKLKDPHLSPVSYADLFGMGNYAMVRI
HHHHHHHHCEEECCCHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHEEEE
LTEHQARTSHLLPNPGSPTKVYLEICHTANLSYPKPLLRHYEHQLSPAIQPMKTLLPLRK
EHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EHLHLIRNNLTTSRFIVAQGCAYKYHQFKINTSGIAKAYAILLPKVPDGCYEYSKGEAYQ
HHHHHHHHCCCHHEEEEECCCEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHCCCCCEEE
IGFGEIRYKLKSSHPLTQLNDKQVIELFNCGINFSSIYNPVNPLQAPLPNRYAFFNQGNL
ECHHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCE
YIMDSPVFIKNDPTLQKFVTSETEKQEGSSETQPYIAFVDKGLPPEDFKEFVEFIHNFGI
EEEECCEEECCCCHHHHHHHCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
QVPKGHVLVLGDNYPMSADSREFGFVPMENLLGSPLCTFWPIGRMGRLTGVSAPTTLSGY
CCCCCCEEEEECCCCCCCCCCCCCCEEHHHHCCCCCHHHCCCCCCCCCCCCCCCCHHHHH
LVSGIALATGLSLIGYVYYQKRRRLFPKKEEKNHKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
>Mature Secondary Structure
MKQHYSLNKSRHILRSTYKLLKSKKLAHSPADKKQLQELLEQLEEAIFEHDQETASDLAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QALAFSNRYPNSFGRKTYELIKALLFAGVVAFLVRQFWFELYEVPTGSMRPTILEQDRIL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEE
VSKTTFGLHCPFAKKPLAFNPESVTRGGLVVFTVGDLPIPDADTKYFGLIPGKKRYIKRC
EEECCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHHHHH
MGRPGDFLYFYGGKIYGLDDAGKRIEFPSVHGLENLYHVPYISFDGTTSSHTEGQKTIID
CCCCCCEEEEECCEEEECCCCCCEECCCCCCCHHHHEECCEEEECCCCCCCCCCCHHHHH
FKQFNQSYGRLIFPQTSMYGQFFDHKEWHQDEPNKLKDPHLSPVSYADLFGMGNYAMVRI
HHHHHHHHCEEECCCHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHEEEE
LTEHQARTSHLLPNPGSPTKVYLEICHTANLSYPKPLLRHYEHQLSPAIQPMKTLLPLRK
EHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EHLHLIRNNLTTSRFIVAQGCAYKYHQFKINTSGIAKAYAILLPKVPDGCYEYSKGEAYQ
HHHHHHHHCCCHHEEEEECCCEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHCCCCCEEE
IGFGEIRYKLKSSHPLTQLNDKQVIELFNCGINFSSIYNPVNPLQAPLPNRYAFFNQGNL
ECHHHEEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEECCCE
YIMDSPVFIKNDPTLQKFVTSETEKQEGSSETQPYIAFVDKGLPPEDFKEFVEFIHNFGI
EEEECCEEECCCCHHHHHHHCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
QVPKGHVLVLGDNYPMSADSREFGFVPMENLLGSPLCTFWPIGRMGRLTGVSAPTTLSGY
CCCCCCEEEEECCCCCCCCCCCCCCEEHHHHCCCCCHHHCCCCCCCCCCCCCCCCHHHHH
LVSGIALATGLSLIGYVYYQKRRRLFPKKEEKNHKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA