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The map label for this gene is murD

Identifier: 15607039

GI number: 15607039

Start: 1460123

End: 1461370

Strand: Direct

Name: murD

Synonym: aq_2075

Alternate gene names: 15607039

Gene position: 1460123-1461370 (Clockwise)

Preceding gene: 15607038

Following gene: 15607041

Centisome position: 94.12

GC content: 42.07

Gene sequence:

>1248_bases
ATGAAGTTCCTCGTCTGGGGACTGGGAAGAAGCGGGAAAGGCGCACTGAAACTTTTGAAGGAGAGAGGATTTGAAGTTTA
CGCAGGAGACGATTCCCAAAATCCAGAGCTCTGGAGAGAAGTTCTCGGTGAAGTGGACACCGTAGTTCTCTCCCCCGGTA
TTCCGCCTTCTCACCCTCTCTGGAAAGAAGCCCTGAAGAAAGAAAAAGAAGTCGTAGGGGAGCTTGAACTCGCATACAGG
TTCTTTAAAGGGAAAGTAATAGCGATTACGGGAACGGACGGGAAGTCCACAACAACGAGACTCACTTACTTAATACTTAA
AAAATTCTTTGACGAAGTCTTTGAAGCCGGAAATATAGGAAAGCCTTTTTCCGAAGTTGTTCTTGAAAATCCAGAAGGTA
TAGCGGTTCTTGAAGTCTCCTCCTTTCAGGGGAAAACCCTAAAGACCTTTAGGCCAAATATTGGGGCCTTTATCAGCTTT
TCTGTGGACCACCTAGACTGGCATCCTAGTATAGAAGATTATTTAAAGAGTAAATACAGGATATTTGAAAACCAAACGGA
AGAGGATTTTTTAATACTGAACGACCTCGTTTATGAAATTAAAAAAACACCAAGCAGGGCAAGAAAAGTACTCTTTTCTG
AGCTATACATAAACTCGGACAGCGTTTTTTACAAAGACATAAGACTTTTTAACCCTAAAAACTTGAAAATAAGGGGACTT
CACAACGTTTACAACGCAAGCGTAGCGTCTCTGATAGCACTTACTCTGGGTTTAAAGCCCGAGGATTTTGAAGAAGTGAT
TTACGAATTTCGCGGTCTTCCACACAGGCTGGAATTTTTGGGAAATTTTAATGGAGTTGAAGTTTATAACGATTCCAAGT
CCACAACTCCCCACGCCTTAATGCACGCTCTGAAAACCTTTCCCGACAATTCAGTTATTCTCATAGTTGGGGGGAAAGAC
AAAGGAGCAGACTTTTACTCCCTTAGGCATATAGTTCAAAAAAAGGTAAAAATCGCCCTTGCAATAGGAGAGACAAAGGA
AAAGATAAAGGACTCTTGGAAGGATATCACCGAGGTGAAAACATGTAACACTCTGGAAGAGGCGGTAAAGCTTGCGAGAG
AAGTATCTAAACTCGGAAATGTAGTTCTCTTTTCGCCCGCCTGTTCTTCCTTTGATATGTTCAGGAATTACGAAGAGAGG
GGAGAGAAGTTTAAGGAACTTGTAGAAATATGGTTCACTCGGACTTAG

Upstream 100 bases:

>100_bases
AGTGGATACTCCACAAAAACAACGTGTTCGTCTGGGAAACGGAAAAGGAAAATCCCTACGAAGCTCTCTCTGAAGTGTCC
CAGATAAAAGAAGCTGTGAC

Downstream 100 bases:

>100_bases
TCCTTTTTATAAACTCCCTTACCTTTTTAAAATCTTTCTTTCCCTTTTCCTTTTCAACTCCCGAAGATACATCTACTCCG
TAAGGTTTTACAAACTCTAT

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKFLVWGLGRSGKGALKLLKERGFEVYAGDDSQNPELWREVLGEVDTVVLSPGIPPSHPLWKEALKKEKEVVGELELAYR
FFKGKVIAITGTDGKSTTTRLTYLILKKFFDEVFEAGNIGKPFSEVVLENPEGIAVLEVSSFQGKTLKTFRPNIGAFISF
SVDHLDWHPSIEDYLKSKYRIFENQTEEDFLILNDLVYEIKKTPSRARKVLFSELYINSDSVFYKDIRLFNPKNLKIRGL
HNVYNASVASLIALTLGLKPEDFEEVIYEFRGLPHRLEFLGNFNGVEVYNDSKSTTPHALMHALKTFPDNSVILIVGGKD
KGADFYSLRHIVQKKVKIALAIGETKEKIKDSWKDITEVKTCNTLEEAVKLAREVSKLGNVVLFSPACSSFDMFRNYEER
GEKFKELVEIWFTRT

Sequences:

>Translated_415_residues
MKFLVWGLGRSGKGALKLLKERGFEVYAGDDSQNPELWREVLGEVDTVVLSPGIPPSHPLWKEALKKEKEVVGELELAYR
FFKGKVIAITGTDGKSTTTRLTYLILKKFFDEVFEAGNIGKPFSEVVLENPEGIAVLEVSSFQGKTLKTFRPNIGAFISF
SVDHLDWHPSIEDYLKSKYRIFENQTEEDFLILNDLVYEIKKTPSRARKVLFSELYINSDSVFYKDIRLFNPKNLKIRGL
HNVYNASVASLIALTLGLKPEDFEEVIYEFRGLPHRLEFLGNFNGVEVYNDSKSTTPHALMHALKTFPDNSVILIVGGKD
KGADFYSLRHIVQKKVKIALAIGETKEKIKDSWKDITEVKTCNTLEEAVKLAREVSKLGNVVLFSPACSSFDMFRNYEER
GEKFKELVEIWFTRT
>Mature_415_residues
MKFLVWGLGRSGKGALKLLKERGFEVYAGDDSQNPELWREVLGEVDTVVLSPGIPPSHPLWKEALKKEKEVVGELELAYR
FFKGKVIAITGTDGKSTTTRLTYLILKKFFDEVFEAGNIGKPFSEVVLENPEGIAVLEVSSFQGKTLKTFRPNIGAFISF
SVDHLDWHPSIEDYLKSKYRIFENQTEEDFLILNDLVYEIKKTPSRARKVLFSELYINSDSVFYKDIRLFNPKNLKIRGL
HNVYNASVASLIALTLGLKPEDFEEVIYEFRGLPHRLEFLGNFNGVEVYNDSKSTTPHALMHALKTFPDNSVILIVGGKD
KGADFYSLRHIVQKKVKIALAIGETKEKIKDSWKDITEVKTCNTLEEAVKLAREVSKLGNVVLFSPACSSFDMFRNYEER
GEKFKELVEIWFTRT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=430, Percent_Identity=30.2325581395349, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_AQUAE (O67852)

Other databases:

- EMBL:   AE000657
- PIR:   H70477
- RefSeq:   NP_214421.1
- ProteinModelPortal:   O67852
- GeneID:   1193841
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2075
- NMPDR:   fig|224324.1.peg.1436
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   O67852
- ProtClustDB:   CLSK2299927
- BioCyc:   AAEO224324:AQ_2075-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47285; Mature: 47285

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLVWGLGRSGKGALKLLKERGFEVYAGDDSQNPELWREVLGEVDTVVLSPGIPPSHPL
CCEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCHH
WKEALKKEKEVVGELELAYRFFKGKVIAITGTDGKSTTTRLTYLILKKFFDEVFEAGNIG
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KPFSEVVLENPEGIAVLEVSSFQGKTLKTFRPNIGAFISFSVDHLDWHPSIEDYLKSKYR
CCHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
IFENQTEEDFLILNDLVYEIKKTPSRARKVLFSELYINSDSVFYKDIRLFNPKNLKIRGL
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCEEEEEEEEECCCCEEEEEH
HNVYNASVASLIALTLGLKPEDFEEVIYEFRGLPHRLEFLGNFNGVEVYNDSKSTTPHAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCHHHH
MHALKTFPDNSVILIVGGKDKGADFYSLRHIVQKKVKIALAIGETKEKIKDSWKDITEVK
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
TCNTLEEAVKLAREVSKLGNVVLFSPACSSFDMFRNYEERGEKFKELVEIWFTRT
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFLVWGLGRSGKGALKLLKERGFEVYAGDDSQNPELWREVLGEVDTVVLSPGIPPSHPL
CCEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCHH
WKEALKKEKEVVGELELAYRFFKGKVIAITGTDGKSTTTRLTYLILKKFFDEVFEAGNIG
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KPFSEVVLENPEGIAVLEVSSFQGKTLKTFRPNIGAFISFSVDHLDWHPSIEDYLKSKYR
CCHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
IFENQTEEDFLILNDLVYEIKKTPSRARKVLFSELYINSDSVFYKDIRLFNPKNLKIRGL
HHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCEEEEEEEEECCCCEEEEEH
HNVYNASVASLIALTLGLKPEDFEEVIYEFRGLPHRLEFLGNFNGVEVYNDSKSTTPHAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCHHHH
MHALKTFPDNSVILIVGGKDKGADFYSLRHIVQKKVKIALAIGETKEKIKDSWKDITEVK
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHH
TCNTLEEAVKLAREVSKLGNVVLFSPACSSFDMFRNYEERGEKFKELVEIWFTRT
HHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320