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The map label for this gene is vacB

Identifier: 15607021

GI number: 15607021

Start: 1438996

End: 1441113

Strand: Direct

Name: vacB

Synonym: aq_2046

Alternate gene names: 15607021

Gene position: 1438996-1441113 (Clockwise)

Preceding gene: 15607018

Following gene: 15607022

Centisome position: 92.76

GC content: 46.79

Gene sequence:

>2118_bases
ATGGACAAAGAAAAACTAGAAAAAGAAGTAATAAAACTCCTGTCAAAGAAGAAAAAACCCCTTCACTTCCTACAGATAGC
TAAAGCCCTTGGGCTCGGGAAGAAGGAGAGGAAGACCTTAAAAAAGGTAATGAGAAAGCTCAAGAAGGAGGGTAAAGTAA
AGGTTGTAAAGGGTAAGTACGAGTACACGGGGGAAGAGGTGGTCACAGGAACCGTTATAGCCTACCCTGGAGGATTCGGA
TTTCTGGAAGTGGAGGGAGGAAAGGATATATACATACCTCCCTTTGAAATGGTAAAAGTTTTTCACGGAGATGTCGTAAA
AGCAAAAGTAACCGAGTTTAAAGGAAAAAAGGAAGTAAGGATAATAAAGGTCCTAAAGAGGGCTAAGAAAGACATAGTGG
CGAAGGTTGTTTTTGAAGACGAGCAGTGCTACGTTGTACCCTTAGACGAAAACGCCCACCACCGTATCCTACTCTCAAAA
AAGGACTGCCAAAAATTAAAGGAAGGTGAAGTAGTTGTCCTTAAGATAACCCAGTTTCCCACGAAGAAATCGCCCGCGAG
GGGGAAAGTTATAGAAGTTCTCGGAAATCCGAAGGAGAAGTTCATAGCGATAGACGTAATCATAAGGAAGTACAACCTAC
CCACATCCTACCCTGAGAAAGTTATTAAGGAAGTTGAGGCAATTCCGGAAGAGATTCCCGAGGAAGAAATAAAGAGGAGA
AGAGATTTAAGGGAGCAGTTGTGCTTTACTATAGACCCCGAGAAGGCAGGGGATTTTGACGACGCTGTGGCGATAGAGCT
CACACCCGAAGGCTACTATAAACTTTACGTCCACATAGCGGATGTGAGTTATTACGTAAGGGAAGGAACGGAAACGGATA
AGGAAGCCTACAAAAGAGGTTTTACGTACTATTTTCCCGACAGGGCTCTCCACATGCTTCCTGAAAAACTCTCCGCAAAA
CTCTGCAGTCTCAGACCCAACGAAGACAAGCTCGCCTTTACCGTTGAGATGGTATTTGACGAGAGCGGAAACCTGAAGGC
TTACGACATTTACGAGAGCGTTATAAGGAGCAAGGCACGCCTCACTTACAACGAAGCCCTTGCCCTTATAGTGGGAGATC
CAGCCCTTGAGAAGAAGTTCCCCAACCTCGTAGAGCCTCTAAGGATGATGGAGACCCTTTACAGGATTTTGAGCAGGAAA
AGGTGGGAGATGGGGAGTATAGACTTTGACCTCCCCGAGGCGGAGGTTATCGTTGATGAATACGGTGAGCCCACCGCTAT
ATACCCCTACGAGAGGCACGTAGCCCACAGGATAATTGAACACTTTATGATATCTGCAAACGAGACGGTAGCCCTGCACC
TTGAACACGCAGGATACCCTTGCCTTTACCGTGTTCACGAACCGCCAGACGAAGAAAAGGTAGAAAACCTCTTGGAAATC
CTCGAAGGACTCGGATATAAAGTGAAGCGTCCACACGAGTACACTCCCAAGTTCTTCCAGAAGATAATAGAGGACTTTGA
AGGAAGACCAGAAGAAAACCTTGTCAGGTTTCTCACCTTAAGAGCTATGGCGAGGGCAAAGTACAGCCCTCACAACGTGG
GACACTTCGGACTTGCCCTTGAACACTACGCCCACTTTACCTCTCCCATCAGGCGTTATCCAGACATAATAGTCCACAGA
CTCCTCAAGAAAGCCCTGAGAGGTGAGGAGATAGACTACGAGAAGACCTTGGCCTACCTCGAAGAGGCGGGAAACCACCT
TTCCAAGCAGGAGAGGATAGCGGACGAGGCGGAAATGGAAGCGATAGATTACCTCAAGGCGAGGTACATGAAGGGAAGGA
TAGGTGAAGAGTTCATCGGAATTATCACGGGAGTTGTTGCCTTCGGATTTTTCGTTGAACTTGAGGAGAATCTTGTTGAG
GGACTCGTGAAGATAAACACGCTCACGGACGATGAGTACGTATTTGACGAACCCGCTCACAGATTAGTTGGTGTGAGGAC
GGGAAAAGTTTTCAGACTCGGCGACCACGTGAAAGTAAGGTGCATAGCGGTGGACGAGGAAAGGGCAAGGGTGGAGTTTG
AACTGATAGAAAAGCTTGAAAAACACGAAACCTTATGA

Upstream 100 bases:

>100_bases
TTTTTTATCCTCTCAAGCGTTGGTTTTACGTCAAAGTCCTTTTCTTTGTATAGTTCCCACAAAATTCCCATGGCGAATAG
AGTTTATAATTTAAAGCATA

Downstream 100 bases:

>100_bases
AAATTTTATAAATAAATGTCTAAGGATAAGGAGAGGAGAAGGAAAATACTCCAGCTTTTGAAGGAAAGGGAAAGGACTCC
TAAGGAACTTGCCACCGTAT

Product: VacB/RNase II family 3'-5' exoribonuclease

Products: NA

Alternate protein names: RNase R; VacB protein homolog

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MDKEKLEKEVIKLLSKKKKPLHFLQIAKALGLGKKERKTLKKVMRKLKKEGKVKVVKGKYEYTGEEVVTGTVIAYPGGFG
FLEVEGGKDIYIPPFEMVKVFHGDVVKAKVTEFKGKKEVRIIKVLKRAKKDIVAKVVFEDEQCYVVPLDENAHHRILLSK
KDCQKLKEGEVVVLKITQFPTKKSPARGKVIEVLGNPKEKFIAIDVIIRKYNLPTSYPEKVIKEVEAIPEEIPEEEIKRR
RDLREQLCFTIDPEKAGDFDDAVAIELTPEGYYKLYVHIADVSYYVREGTETDKEAYKRGFTYYFPDRALHMLPEKLSAK
LCSLRPNEDKLAFTVEMVFDESGNLKAYDIYESVIRSKARLTYNEALALIVGDPALEKKFPNLVEPLRMMETLYRILSRK
RWEMGSIDFDLPEAEVIVDEYGEPTAIYPYERHVAHRIIEHFMISANETVALHLEHAGYPCLYRVHEPPDEEKVENLLEI
LEGLGYKVKRPHEYTPKFFQKIIEDFEGRPEENLVRFLTLRAMARAKYSPHNVGHFGLALEHYAHFTSPIRRYPDIIVHR
LLKKALRGEEIDYEKTLAYLEEAGNHLSKQERIADEAEMEAIDYLKARYMKGRIGEEFIGIITGVVAFGFFVELEENLVE
GLVKINTLTDDEYVFDEPAHRLVGVRTGKVFRLGDHVKVRCIAVDEERARVEFELIEKLEKHETL

Sequences:

>Translated_705_residues
MDKEKLEKEVIKLLSKKKKPLHFLQIAKALGLGKKERKTLKKVMRKLKKEGKVKVVKGKYEYTGEEVVTGTVIAYPGGFG
FLEVEGGKDIYIPPFEMVKVFHGDVVKAKVTEFKGKKEVRIIKVLKRAKKDIVAKVVFEDEQCYVVPLDENAHHRILLSK
KDCQKLKEGEVVVLKITQFPTKKSPARGKVIEVLGNPKEKFIAIDVIIRKYNLPTSYPEKVIKEVEAIPEEIPEEEIKRR
RDLREQLCFTIDPEKAGDFDDAVAIELTPEGYYKLYVHIADVSYYVREGTETDKEAYKRGFTYYFPDRALHMLPEKLSAK
LCSLRPNEDKLAFTVEMVFDESGNLKAYDIYESVIRSKARLTYNEALALIVGDPALEKKFPNLVEPLRMMETLYRILSRK
RWEMGSIDFDLPEAEVIVDEYGEPTAIYPYERHVAHRIIEHFMISANETVALHLEHAGYPCLYRVHEPPDEEKVENLLEI
LEGLGYKVKRPHEYTPKFFQKIIEDFEGRPEENLVRFLTLRAMARAKYSPHNVGHFGLALEHYAHFTSPIRRYPDIIVHR
LLKKALRGEEIDYEKTLAYLEEAGNHLSKQERIADEAEMEAIDYLKARYMKGRIGEEFIGIITGVVAFGFFVELEENLVE
GLVKINTLTDDEYVFDEPAHRLVGVRTGKVFRLGDHVKVRCIAVDEERARVEFELIEKLEKHETL
>Mature_705_residues
MDKEKLEKEVIKLLSKKKKPLHFLQIAKALGLGKKERKTLKKVMRKLKKEGKVKVVKGKYEYTGEEVVTGTVIAYPGGFG
FLEVEGGKDIYIPPFEMVKVFHGDVVKAKVTEFKGKKEVRIIKVLKRAKKDIVAKVVFEDEQCYVVPLDENAHHRILLSK
KDCQKLKEGEVVVLKITQFPTKKSPARGKVIEVLGNPKEKFIAIDVIIRKYNLPTSYPEKVIKEVEAIPEEIPEEEIKRR
RDLREQLCFTIDPEKAGDFDDAVAIELTPEGYYKLYVHIADVSYYVREGTETDKEAYKRGFTYYFPDRALHMLPEKLSAK
LCSLRPNEDKLAFTVEMVFDESGNLKAYDIYESVIRSKARLTYNEALALIVGDPALEKKFPNLVEPLRMMETLYRILSRK
RWEMGSIDFDLPEAEVIVDEYGEPTAIYPYERHVAHRIIEHFMISANETVALHLEHAGYPCLYRVHEPPDEEKVENLLEI
LEGLGYKVKRPHEYTPKFFQKIIEDFEGRPEENLVRFLTLRAMARAKYSPHNVGHFGLALEHYAHFTSPIRRYPDIIVHR
LLKKALRGEEIDYEKTLAYLEEAGNHLSKQERIADEAEMEAIDYLKARYMKGRIGEEFIGIITGVVAFGFFVELEENLVE
GLVKINTLTDDEYVFDEPAHRLVGVRTGKVFRLGDHVKVRCIAVDEERARVEFELIEKLEKHETL

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI190014625, Length=531, Percent_Identity=29.3785310734463, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI190014623, Length=531, Percent_Identity=29.3785310734463, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI134288890, Length=446, Percent_Identity=30.2690582959641, Blast_Score=185, Evalue=2e-46,
Organism=Homo sapiens, GI19115966, Length=544, Percent_Identity=26.8382352941176, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI219521928, Length=544, Percent_Identity=26.8382352941176, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI156105695, Length=375, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI156105693, Length=374, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI87082383, Length=704, Percent_Identity=37.5, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1787542, Length=583, Percent_Identity=28.4734133790738, Blast_Score=199, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI212645896, Length=575, Percent_Identity=29.2173913043478, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17553506, Length=463, Percent_Identity=31.7494600431965, Blast_Score=189, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324552, Length=461, Percent_Identity=31.0195227765727, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320499, Length=464, Percent_Identity=22.8448275862069, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24649634, Length=512, Percent_Identity=29.1015625, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19922976, Length=367, Percent_Identity=31.8801089918256, Blast_Score=174, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24654597, Length=367, Percent_Identity=31.8801089918256, Blast_Score=174, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24654592, Length=367, Percent_Identity=31.8801089918256, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNR_AQUAE (O67834)

Other databases:

- EMBL:   AE000657
- PIR:   F70475
- RefSeq:   NP_214403.1
- ProteinModelPortal:   O67834
- SMR:   O67834
- GeneID:   1193731
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2046
- NMPDR:   fig|224324.1.peg.1418
- HOGENOM:   HBG690243
- OMA:   GHRDGFG
- PhylomeDB:   O67834
- ProtClustDB:   CLSK2299917
- BioCyc:   AAEO224324:AQ_2046-MONOMER
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR23355:SF9
- SMART:   SM00357
- SMART:   SM00955
- SMART:   SM00316
- TIGRFAMs:   TIGR00358
- TIGRFAMs:   TIGR02063

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 3.1.-.- [C]

Molecular weight: Translated: 81539; Mature: 81539

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS01175 RIBONUCLEASE_II; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKEKLEKEVIKLLSKKKKPLHFLQIAKALGLGKKERKTLKKVMRKLKKEGKVKVVKGKY
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
EYTGEEVVTGTVIAYPGGFGFLEVEGGKDIYIPPFEMVKVFHGDVVKAKVTEFKGKKEVR
EECCCEEEEEEEEEECCCCEEEEECCCCEEECCHHHHHHHHCCCHHEEHHHHCCCCCHHH
IIKVLKRAKKDIVAKVVFEDEQCYVVPLDENAHHRILLSKKDCQKLKEGEVVVLKITQFP
HHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
TKKSPARGKVIEVLGNPKEKFIAIDVIIRKYNLPTSYPEKVIKEVEAIPEEIPEEEIKRR
CCCCCCCCCEEEECCCCHHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
RDLREQLCFTIDPEKAGDFDDAVAIELTPEGYYKLYVHIADVSYYVREGTETDKEAYKRG
HHHHHHHCEEECCCCCCCCCCEEEEEECCCCEEEEEEEEECHHHHHHCCCCCHHHHHHCC
FTYYFPDRALHMLPEKLSAKLCSLRPNEDKLAFTVEMVFDESGNLKAYDIYESVIRSKAR
CEEECCCHHHHHCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHH
LTYNEALALIVGDPALEKKFPNLVEPLRMMETLYRILSRKRWEMGSIDFDLPEAEVIVDE
EEHHHEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCC
YGEPTAIYPYERHVAHRIIEHFMISANETVALHLEHAGYPCLYRVHEPPDEEKVENLLEI
CCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHH
LEGLGYKVKRPHEYTPKFFQKIIEDFEGRPEENLVRFLTLRAMARAKYSPHNVGHFGLAL
HHCCCCEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
EHYAHFTSPIRRYPDIIVHRLLKKALRGEEIDYEKTLAYLEEAGNHLSKQERIADEAEME
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDYLKARYMKGRIGEEFIGIITGVVAFGFFVELEENLVEGLVKINTLTDDEYVFDEPAH
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCHH
RLVGVRTGKVFRLGDHVKVRCIAVDEERARVEFELIEKLEKHETL
HHEEECCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKEKLEKEVIKLLSKKKKPLHFLQIAKALGLGKKERKTLKKVMRKLKKEGKVKVVKGKY
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
EYTGEEVVTGTVIAYPGGFGFLEVEGGKDIYIPPFEMVKVFHGDVVKAKVTEFKGKKEVR
EECCCEEEEEEEEEECCCCEEEEECCCCEEECCHHHHHHHHCCCHHEEHHHHCCCCCHHH
IIKVLKRAKKDIVAKVVFEDEQCYVVPLDENAHHRILLSKKDCQKLKEGEVVVLKITQFP
HHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
TKKSPARGKVIEVLGNPKEKFIAIDVIIRKYNLPTSYPEKVIKEVEAIPEEIPEEEIKRR
CCCCCCCCCEEEECCCCHHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
RDLREQLCFTIDPEKAGDFDDAVAIELTPEGYYKLYVHIADVSYYVREGTETDKEAYKRG
HHHHHHHCEEECCCCCCCCCCEEEEEECCCCEEEEEEEEECHHHHHHCCCCCHHHHHHCC
FTYYFPDRALHMLPEKLSAKLCSLRPNEDKLAFTVEMVFDESGNLKAYDIYESVIRSKAR
CEEECCCHHHHHCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHH
LTYNEALALIVGDPALEKKFPNLVEPLRMMETLYRILSRKRWEMGSIDFDLPEAEVIVDE
EEHHHEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCC
YGEPTAIYPYERHVAHRIIEHFMISANETVALHLEHAGYPCLYRVHEPPDEEKVENLLEI
CCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHH
LEGLGYKVKRPHEYTPKFFQKIIEDFEGRPEENLVRFLTLRAMARAKYSPHNVGHFGLAL
HHCCCCEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
EHYAHFTSPIRRYPDIIVHRLLKKALRGEEIDYEKTLAYLEEAGNHLSKQERIADEAEME
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDYLKARYMKGRIGEEFIGIITGVVAFGFFVELEENLVEGLVKINTLTDDEYVFDEPAH
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCHH
RLVGVRTGKVFRLGDHVKVRCIAVDEERARVEFELIEKLEKHETL
HHEEECCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320