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The map label for this gene is tufA2 [H]

Identifier: 15606942

GI number: 15606942

Start: 1356932

End: 1358149

Strand: Direct

Name: tufA2 [H]

Synonym: aq_1928

Alternate gene names: 15606942

Gene position: 1356932-1358149 (Clockwise)

Preceding gene: 15606939

Following gene: 15606943

Centisome position: 87.47

GC content: 50.0

Gene sequence:

>1218_bases
ATGGCAAAGGAGAAATTTGAAAGGACAAAAGAACACGTAAACGTGGGAACAATAGGACACGTTGACCACGGCAAAAGCAC
TCTCACTTCCGCTATAACATGCGTTCTCGCTGCAGGCCTCGTTGAAGGCGGTAAAGCTAAGTGCTTCAAATACGAAGAAA
TCGACAAGGCTCCAGAAGAAAAAGAAAGAGGAATCACGATAAACATCACCCACGTTGAGTACGAAACCGCCAAACGCCAC
TACGCTCACGTTGACTGCCCCGGTCACGCTGACTACATCAAGAATATGATCACCGGCGCAGCTCAAATGGACGGTGCAAT
ACTCGTTGTTTCCGCAGCAGACGGCCCCATGCCCCAAACTAGAGAACACGTGCTTCTCGCAAGACAGGTTAACGTTCCTT
ACATAGTCGTTTTCATGAGCAAGTGTGATATGGTTGACGACGAAGAACTTTTAGAACTCGTTGAGCTCGAAGTTAGAGAA
CTTCTCTCCAAGTACGAGTATCCCGGAGACGAAGTTCCCGTTATCAGAGGATCGGCACTTGGAGCACTTCAAGAACTCGA
ACAAAACTCTCCCGGCAAGTGGGTTGAGTCCATAAAAGAGCTTCTCAACGCGATGGACGAGTACATACCGACACCACAAA
GGGAAGTGGACAAGCCCTTCCTTATGCCAATAGAAGACGTGTTTAGCATATCCGGAAGAGGAACGGTTGTAACCGGAAGA
GTAGAGAGAGGAGTTCTGAGGCCCGGAGACGAAGTAGAGATAGTAGGACTCAGGGAAGAGCCCCTCAAGACAGTTGCAAC
GTCTATAGAGATGTTCAGGAAGGTGCTTGACGAGGCACTGCCCGGAGACAACATAGGAGTGCTGCTGAGGGGAGTAGGAA
GGGACGACGTAGAAAGGGGACAGGTATTGGCGCAACCTGGGAGTGTAAAGGCTCACAAGAGGTTCAGGGCACAGGTATAT
GTACTTAGCAAGGAGGAAGGAGGAAGACACACTCCGTTCTTTGTGAACTACAGACCGCAGTTTTACTTCAGGACGGCTGA
CGTGACGGGGACTGTGGTGAAGCTTCCAGAAGGAGTGGAGATGGTGATGCCTGGGGACAACGTAGAGCTTGAGGTGGAGC
TTATAGCACCTGTGGCGTTGGAAGAAGGGCTCAGGTTTGCGATAAGGGAAGGTGGTAGAACAGTAGGTGCAGGTGTCGTT
ACTAAAATACTGGATTGA

Upstream 100 bases:

>100_bases
GTCCGGTCCTGGGCTCCACTTCCCTGATAAAAATCCCTCTTTACAAACGTAAAAGAAATAATTTATAATATTTTGTTACC
TCAAAATTCGGAGGTAGGAA

Downstream 100 bases:

>100_bases
GGTTTGAAAAATGGCTAGGGAAATAATTACTCTCGCTTGTACCGAATGCAAGAGGAGGAACTACACCACTACGAAGAACA
AGCAGAAGCACCCCGAGAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRH
YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMSKCDMVDDEELLELVELEVRE
LLSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGRDDVERGQVLAQPGSVKAHKRFRAQVY
VLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVV
TKILD

Sequences:

>Translated_405_residues
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRH
YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMSKCDMVDDEELLELVELEVRE
LLSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGRDDVERGQVLAQPGSVKAHKRFRAQVY
VLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVV
TKILD
>Mature_404_residues
AKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRHY
AHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMSKCDMVDDEELLELVELEVREL
LSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGRV
ERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGRDDVERGQVLAQPGSVKAHKRFRAQVYV
LSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVT
KILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=403, Percent_Identity=56.8238213399504, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=32.3725055432373, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=32.5112107623318, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=31.6027088036117, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=31.6027088036117, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=29.512893982808, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=29.512893982808, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=29.512893982808, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=28.8571428571429, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=25.7403189066059, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI4503507, Length=348, Percent_Identity=27.2988505747126, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI45593140, Length=334, Percent_Identity=25.4491017964072, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=258, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729339, Length=280, Percent_Identity=29.6428571428571, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI53729337, Length=280, Percent_Identity=29.6428571428571, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=404, Percent_Identity=70.049504950495, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1789737, Length=404, Percent_Identity=70.049504950495, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI2367247, Length=397, Percent_Identity=26.9521410579345, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994988, Length=248, Percent_Identity=29.8387096774194, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789108, Length=312, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=408, Percent_Identity=54.4117647058823, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=398, Percent_Identity=45.2261306532663, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32566303, Length=449, Percent_Identity=31.4031180400891, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=27.3522975929978, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=27.3972602739726, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=27.3972602739726, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=253, Percent_Identity=31.6205533596838, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=161, Percent_Identity=41.6149068322981, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=331, Percent_Identity=25.9818731117825, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=434, Percent_Identity=24.6543778801843, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17563834, Length=433, Percent_Identity=24.4803695150115, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=404, Percent_Identity=65.0990099009901, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=31.6258351893096, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=31.6258351893096, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320377, Length=444, Percent_Identity=25.6756756756757, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.3561253561254, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=340, Percent_Identity=26.4705882352941, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=253, Percent_Identity=26.8774703557312, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363316, Length=402, Percent_Identity=58.2089552238806, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=402, Percent_Identity=58.2089552238806, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=397, Percent_Identity=48.8664987405542, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652838, Length=459, Percent_Identity=32.4618736383442, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI17137572, Length=459, Percent_Identity=32.4618736383442, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI45553807, Length=459, Percent_Identity=32.244008714597, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45553816, Length=459, Percent_Identity=32.244008714597, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24651721, Length=459, Percent_Identity=32.244008714597, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI17864154, Length=459, Percent_Identity=32.244008714597, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=31.1428571428571, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.9816513761468, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922690, Length=312, Percent_Identity=30.4487179487179, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24647048, Length=360, Percent_Identity=25.2777777777778, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24647046, Length=360, Percent_Identity=25.2777777777778, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78706572, Length=257, Percent_Identity=26.0700389105058, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 44745; Mature: 44614

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEE
CCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCH
KERGITINITHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT
HHCCEEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC
REHVLLARQVNVPYIVVFMSKCDMVDDEELLELVELEVRELLSKYEYPGDEVPVIRGSAL
HHHEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
GALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECH
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGRDDVERG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCC
QVLAQPGSVKAHKRFRAQVYVLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVE
EEEECCCCCHHHHEEEEEEEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCE
MVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVTKILD
EEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEE
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCH
KERGITINITHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT
HHCCEEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC
REHVLLARQVNVPYIVVFMSKCDMVDDEELLELVELEVRELLSKYEYPGDEVPVIRGSAL
HHHEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
GALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECH
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGRDDVERG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCC
QVLAQPGSVKAHKRFRAQVYVLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVE
EEEECCCCCHHHHEEEEEEEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCE
MVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVTKILD
EEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]