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The map label for this gene is sbcD [H]

Identifier: 15606914

GI number: 15606914

Start: 1327901

End: 1329040

Strand: Direct

Name: sbcD [H]

Synonym: aq_1886

Alternate gene names: 15606914

Gene position: 1327901-1329040 (Clockwise)

Preceding gene: 15606913

Following gene: 15606915

Centisome position: 85.6

GC content: 43.68

Gene sequence:

>1140_bases
ATGAGGCTTATACATCTGTCTGACATCCACGCGGGAAAGAATCTCGGGCGTGTCTCGAGAAACGAAGACGTAGTTTACGC
ACTCAATCAGGTAGTAGACTTTTGCAAAGAAAACAAACCCGATTTGGTCCTCGTTGCGGGAGACGTTTTCGATAAGGCTA
ACCCCGATAACGAGGCGAAAGAGATTATTTTCGACTTCTTCTTAAAGCTCCACTCCTTAAATATCCCGTCGGTTGTTATA
TCGGGAAACCACGACAGTTACGAGTTTATGAAGAGTTTAAAAAAACTCCTTCAAGTTATAGACGTGTACGTCGTTCCCAA
ACCGAACTTAGAGGAGTGCGTACTTGATGTAAAAGGTCTAAAAATCGCATGCCTCCCATACCCTTCCGAGAGGGTTTTAA
CGAGGGCGGGCGAGGATTCTAAACTCTCATACGCAGTTCTCGTGGAAAAGGCCATCAAGTATCTTTACGAAAAGGTAAAA
GATGCACCTCTTAAGGTACTCCTTTCTCACCTTTTTATAGCGGGTTCAAAGTACACGAGAACCGAGAGGGAGGCTACTAT
CACAGATTACTACGCGGTAGAACCTTCCGCAATTCCCGAAGGCTTTGATTACGTAGCCCTCGGACACGTACACAGGTATC
AGAGGATAGAAAAAGCACCAACCCACGTTTACTACACCGGTTCCCTCTTTCAGCTTGACTTTTCTGAAGCCGGGCAGGAA
AAGTTTTTTAACTTCGTAGAATTGAAGGAAGGAGAGCCTCCCCACGTTGAAGCGATAAAACTCTCACTGAAGAACCCACT
TCACACCTTTGAAATAAACCAAGAAAATTACGCAAAGGAACTCGAAAAAATAAAAGACGTTCAGGGCTACATCAAGGTTC
ACTTAATCGTAAAGGATAAGACGAAGATGCACCTTGTAGCTGAAAAGGTAAAGGAAGCACTAGGGGAGAAACTCATAAAA
CTTGAACTCCTGACTCCGGAAGAAGCCATTAAAAAGAGACTGGAAGAGGAGAGGGAAATCCAGAAATTGAACTTACTCGA
CCTTTACAGGGATTACTTCAAAGAAAGTTACGGAAGGGAAGTCCCTCAAGAAATAATAAAAACCGTTTCCCAGTTATTAG
AGCAGGTACATGAATCCTGA

Upstream 100 bases:

>100_bases
GTAAAGCACATAATGAACAAAGCGAGGGAAAAGTACTTCCACTTGAAAAAGTTCGGAGAGACTTCCTTTCTGAGAAAACT
TGAGAGAATATTAAAGAATT

Downstream 100 bases:

>100_bases
AAGCAGGGTAATAAGGAAAGTTTTAGCCCTTCAGAACGACGAAAAGATTTTTAGCGGAGAAAGAAGGGTTTTAATCGCCT
TCTCGGGCGGGGTGGACTCC

Product: ATP-dependent dsDNA exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRLIHLSDIHAGKNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEAKEIIFDFFLKLHSLNIPSVVI
SGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKGLKIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVK
DAPLKVLLSHLFIAGSKYTRTEREATITDYYAVEPSAIPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQLDFSEAGQE
KFFNFVELKEGEPPHVEAIKLSLKNPLHTFEINQENYAKELEKIKDVQGYIKVHLIVKDKTKMHLVAEKVKEALGEKLIK
LELLTPEEAIKKRLEEEREIQKLNLLDLYRDYFKESYGREVPQEIIKTVSQLLEQVHES

Sequences:

>Translated_379_residues
MRLIHLSDIHAGKNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEAKEIIFDFFLKLHSLNIPSVVI
SGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKGLKIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVK
DAPLKVLLSHLFIAGSKYTRTEREATITDYYAVEPSAIPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQLDFSEAGQE
KFFNFVELKEGEPPHVEAIKLSLKNPLHTFEINQENYAKELEKIKDVQGYIKVHLIVKDKTKMHLVAEKVKEALGEKLIK
LELLTPEEAIKKRLEEEREIQKLNLLDLYRDYFKESYGREVPQEIIKTVSQLLEQVHES
>Mature_379_residues
MRLIHLSDIHAGKNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEAKEIIFDFFLKLHSLNIPSVVI
SGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKGLKIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVK
DAPLKVLLSHLFIAGSKYTRTEREATITDYYAVEPSAIPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQLDFSEAGQE
KFFNFVELKEGEPPHVEAIKLSLKNPLHTFEINQENYAKELEKIKDVQGYIKVHLIVKDKTKMHLVAEKVKEALGEKLIK
LELLTPEEAIKKRLEEEREIQKLNLLDLYRDYFKESYGREVPQEIIKTVSQLLEQVHES

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=355, Percent_Identity=26.7605633802817, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 43590; Mature: 43590

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIHLSDIHAGKNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEAK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
EIIFDFFLKLHSLNIPSVVISGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKGL
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCC
KIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVKDAPLKVLLSHLFIAGSKYTR
EEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
TEREATITDYYAVEPSAIPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQLDFSEAGQE
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEHHHCCHH
KFFNFVELKEGEPPHVEAIKLSLKNPLHTFEINQENYAKELEKIKDVQGYIKVHLIVKDK
HHHHHHEECCCCCCCHHHEEEECCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEEEEECC
TKMHLVAEKVKEALGEKLIKLELLTPEEAIKKRLEEEREIQKLNLLDLYRDYFKESYGRE
HHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPQEIIKTVSQLLEQVHES
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLIHLSDIHAGKNLGRVSRNEDVVYALNQVVDFCKENKPDLVLVAGDVFDKANPDNEAK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
EIIFDFFLKLHSLNIPSVVISGNHDSYEFMKSLKKLLQVIDVYVVPKPNLEECVLDVKGL
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCC
KIACLPYPSERVLTRAGEDSKLSYAVLVEKAIKYLYEKVKDAPLKVLLSHLFIAGSKYTR
EEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
TEREATITDYYAVEPSAIPEGFDYVALGHVHRYQRIEKAPTHVYYTGSLFQLDFSEAGQE
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEHHHCCHH
KFFNFVELKEGEPPHVEAIKLSLKNPLHTFEINQENYAKELEKIKDVQGYIKVHLIVKDK
HHHHHHEECCCCCCCHHHEEEECCCCCEEEEECHHHHHHHHHHHHHHCCEEEEEEEEECC
TKMHLVAEKVKEALGEKLIKLELLTPEEAIKKRLEEEREIQKLNLLDLYRDYFKESYGRE
HHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPQEIIKTVSQLLEQVHES
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]