Click here to switch to the map view.

The map label for this gene is yjbJ [H]

Identifier: 15606851

GI number: 15606851

Start: 1268039

End: 1269118

Strand: Direct

Name: yjbJ [H]

Synonym: aq_1796

Alternate gene names: 15606851

Gene position: 1268039-1269118 (Clockwise)

Preceding gene: 15606850

Following gene: 15606863

Centisome position: 81.74

GC content: 40.83

Gene sequence:

>1080_bases
TTGATTTACGTTTACCTAAAGCAACGTGCCTATAAAAAAGCCGAAAAATTTGTAAGGAGTAAGAAAAATCCTGAGCTTTA
CTTTATCCTTGGAAGGGAGTTATTAGAAAGGGGACTTGCGAACAGAGCTATTACTTTTCTGAAAAACTCTATGAATACGG
AAGCGAACTTTTCCCTTGGCTTGGCTTACTACCTGAAAAAAGACTACGAGAAGGCTAAACACTACTTTTTGAGCTACCAT
CCGCCAGACGAACTGGGAAAAGGAAAAAGAGATTTCTGGCTTTTTAAAACGTATCTTCAGCTGGGAAACTCCGAGAAAGC
TCTTGATTACTTAAAAAAGGCTTCTTCTTACGATAACTTCTACGGAGCTGTTTCAAGAAAGTTATTAGGTTTGAGGGTGT
ATAAACCTGTTCAGTTTTCTTCCGTTGAACCGCCGAGGTTGTTTCCCGAACTTTACAGAATAAGAGAATTGGGATTTTTG
TCTTACATGCGATATGAAGCTTTCAGGCATGAACACCTGGCTACCACGGGAGATGTCCTTTTGATTTCAAGGATTGATCC
GTACACGTCAATAAGACTCGCCGTAAAAAAGTACGGTGTGAAGTCGGAAATATACAGGGTGGTTTCTCATCCCACTCCCT
ACGAAAATCTGGTTTTTGAAGCTGCCCAAAAGTTTGAAGTACCGGTTCCCCTTATATACGCAGTTATGAGACAGGAAAGT
TTATTTGATCCGTACGCTGTTTCCTCTTCAGGAGCAAAGGGGTTGATGCAGTTGATTGATTCAACTGCGAGGTGGAAGGC
GAAAAGGCTCGGTTTAAAGATCAGGAGCGTGTTTGATCCAGAGACGAATATAACACTCGGTACAGCTTACCTGGCTTTTT
TAATGGATTACTGGAACGGAGATTTAATTAGAGTTATTGCTTCTTACAACGCCGGACATGGAGCCGTTAGTAGATGGAAG
AGGTACGAGGACGACTTCTTATTTATAGAACTGATTCCCTACAACGAAACGAGGAATTACGTAAAAAGGGTTTTATACAA
TTACTACGTTTATTCGGAAAAGTTAGAAAAGGGTCTCTGA

Upstream 100 bases:

>100_bases
AGGACTGTTACCTCAAGGGAAAAACTTATTACAGATTAGAAAAGTACAAAATAGCGGAAAGGATATTAAAAAACTGTCCA
AAGAAAGAGCCCAAAAGTTT

Downstream 100 bases:

>100_bases
CTCTTCCAGTTCTTCTACTGGTCTTCTTCCCGCAAAGCCTTCGTTCAATTCGTGCCAGTACATAATCGTTTCTTCATCTT
CCTTCCAGCATAGCCATATG

Product: hypothetical protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIYVYLKQRAYKKAEKFVRSKKNPELYFILGRELLERGLANRAITFLKNSMNTEANFSLGLAYYLKKDYEKAKHYFLSYH
PPDELGKGKRDFWLFKTYLQLGNSEKALDYLKKASSYDNFYGAVSRKLLGLRVYKPVQFSSVEPPRLFPELYRIRELGFL
SYMRYEAFRHEHLATTGDVLLISRIDPYTSIRLAVKKYGVKSEIYRVVSHPTPYENLVFEAAQKFEVPVPLIYAVMRQES
LFDPYAVSSSGAKGLMQLIDSTARWKAKRLGLKIRSVFDPETNITLGTAYLAFLMDYWNGDLIRVIASYNAGHGAVSRWK
RYEDDFLFIELIPYNETRNYVKRVLYNYYVYSEKLEKGL

Sequences:

>Translated_359_residues
MIYVYLKQRAYKKAEKFVRSKKNPELYFILGRELLERGLANRAITFLKNSMNTEANFSLGLAYYLKKDYEKAKHYFLSYH
PPDELGKGKRDFWLFKTYLQLGNSEKALDYLKKASSYDNFYGAVSRKLLGLRVYKPVQFSSVEPPRLFPELYRIRELGFL
SYMRYEAFRHEHLATTGDVLLISRIDPYTSIRLAVKKYGVKSEIYRVVSHPTPYENLVFEAAQKFEVPVPLIYAVMRQES
LFDPYAVSSSGAKGLMQLIDSTARWKAKRLGLKIRSVFDPETNITLGTAYLAFLMDYWNGDLIRVIASYNAGHGAVSRWK
RYEDDFLFIELIPYNETRNYVKRVLYNYYVYSEKLEKGL
>Mature_359_residues
MIYVYLKQRAYKKAEKFVRSKKNPELYFILGRELLERGLANRAITFLKNSMNTEANFSLGLAYYLKKDYEKAKHYFLSYH
PPDELGKGKRDFWLFKTYLQLGNSEKALDYLKKASSYDNFYGAVSRKLLGLRVYKPVQFSSVEPPRLFPELYRIRELGFL
SYMRYEAFRHEHLATTGDVLLISRIDPYTSIRLAVKKYGVKSEIYRVVSHPTPYENLVFEAAQKFEVPVPLIYAVMRQES
LFDPYAVSSSGAKGLMQLIDSTARWKAKRLGLKIRSVFDPETNITLGTAYLAFLMDYWNGDLIRVIASYNAGHGAVSRWK
RYEDDFLFIELIPYNETRNYVKRVLYNYYVYSEKLEKGL

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=148, Percent_Identity=37.8378378378378, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082191, Length=118, Percent_Identity=43.2203389830509, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42100; Mature: 42100

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYVYLKQRAYKKAEKFVRSKKNPELYFILGRELLERGLANRAITFLKNSMNTEANFSLG
CEEEEEHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LAYYLKKDYEKAKHYFLSYHPPDELGKGKRDFWLFKTYLQLGNSEKALDYLKKASSYDNF
HHHHHHHHHHHHHHEEEEECCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
YGAVSRKLLGLRVYKPVQFSSVEPPRLFPELYRIRELGFLSYMRYEAFRHEHLATTGDVL
HHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LISRIDPYTSIRLAVKKYGVKSEIYRVVSHPTPYENLVFEAAQKFEVPVPLIYAVMRQES
EEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LFDPYAVSSSGAKGLMQLIDSTARWKAKRLGLKIRSVFDPETNITLGTAYLAFLMDYWNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHHCC
DLIRVIASYNAGHGAVSRWKRYEDDFLFIELIPYNETRNYVKRVLYNYYVYSEKLEKGL
CCEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYVYLKQRAYKKAEKFVRSKKNPELYFILGRELLERGLANRAITFLKNSMNTEANFSLG
CEEEEEHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LAYYLKKDYEKAKHYFLSYHPPDELGKGKRDFWLFKTYLQLGNSEKALDYLKKASSYDNF
HHHHHHHHHHHHHHEEEEECCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
YGAVSRKLLGLRVYKPVQFSSVEPPRLFPELYRIRELGFLSYMRYEAFRHEHLATTGDVL
HHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LISRIDPYTSIRLAVKKYGVKSEIYRVVSHPTPYENLVFEAAQKFEVPVPLIYAVMRQES
EEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LFDPYAVSSSGAKGLMQLIDSTARWKAKRLGLKIRSVFDPETNITLGTAYLAFLMDYWNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHHHHHHHHCC
DLIRVIASYNAGHGAVSRWKRYEDDFLFIELIPYNETRNYVKRVLYNYYVYSEKLEKGL
CCEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]