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The map label for this gene is pol [C]

Identifier: 15606735

GI number: 15606735

Start: 1141158

End: 1142027

Strand: Direct

Name: pol [C]

Synonym: aq_1628

Alternate gene names: 15606735

Gene position: 1141158-1142027 (Clockwise)

Preceding gene: 15606734

Following gene: 15606740

Centisome position: 73.56

GC content: 39.2

Gene sequence:

>870_bases
ATGAAAACCCTCTACATTTTGGACGGCTCTTCATTCGTTTACAGAAGTTTCTTCGCACTCCCTCCCCTCAGTACAAGTAA
AGGTTTTCCCACGAACGCTATATACGGTTTCTTGAGGATGTTATTTTCCCTTATTAAGAAAGAAAGACCTCAGTACTTGG
TCGTGGTCTTTGATGCTCCCGCAAAGACAAAAAGGGAAAAAATATACGCAGACTATAAAAAACAAAGACCGAAAGCCCCG
GATCCACTAAAGGTTCAAATTCCCGTAATAAAGGAAATACTAAAACTCGCCGGTATACCCCTTTTAGAACTTCCCGGATA
CGAAGCAGACGACGTTATAGCTTACTTGGCAGAAAAATTTTCCCAAAAAGGTTTTAAAGTAAAGATTTACTCCCCGGACA
AAGACCTTTTACAGCTCGTGTCCGAAAACGTTCTGGTCATAAACCCTATGAACGATGAAGTGTTCACAAAAGAAAGAGTA
ATAAAGAAGTTCGGAGTAGAACCCCAAAAAATACCAGACTACCTCGCACTCGTAGGAGATAAAGTGGACAACGTCCCCGG
AATAGAAGGCGTAGGACCAAAAACGGCTATAAACATACTTAAAAAGTACGGCAGTGTGGAAAATATCTTAAAAAACTGGG
AAAAGTTTCAAAGAGAGTTTCCAAGGGCAAAAAAAGAAGACTTAGAACTCTCCTACAAGCTCGTAAAACTCTACACGGAT
TTAGATATTGAACTCTCCGAAGAAGATTTAAAGATAAAACGACCTGATCTGAATAAACTAAAACAAAAACTTCAAGAACT
TGAGATGAAGAGCCTTTTAAAAGAAGTGGATAAGATTTTCAGACAGGCTTCTCAGAGATCGTTGTTTTGA

Upstream 100 bases:

>100_bases
AGACATTAAAAAGCTCGAGAGTATACTATTTGGAAGAGCCCCGATTCCCAAAAAAACGGGGGAAGATATAGGAAGCTTTA
AGTGTTAAATTAAAAATTTC

Downstream 100 bases:

>100_bases
TATATCTCCCTCAGAACACTCAAATTCACCGGATCCATGTTATTCTCTTTGGGAGTTTCTATGTAGTAGGGAAGTGTTGA
AAAGTACTCATCCTTCAGAA

Product: DNA polymerase I

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKTLYILDGSSFVYRSFFALPPLSTSKGFPTNAIYGFLRMLFSLIKKERPQYLVVVFDAPAKTKREKIYADYKKQRPKAP
DPLKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKFSQKGFKVKIYSPDKDLLQLVSENVLVINPMNDEVFTKERV
IKKFGVEPQKIPDYLALVGDKVDNVPGIEGVGPKTAINILKKYGSVENILKNWEKFQREFPRAKKEDLELSYKLVKLYTD
LDIELSEEDLKIKRPDLNKLKQKLQELEMKSLLKEVDKIFRQASQRSLF

Sequences:

>Translated_289_residues
MKTLYILDGSSFVYRSFFALPPLSTSKGFPTNAIYGFLRMLFSLIKKERPQYLVVVFDAPAKTKREKIYADYKKQRPKAP
DPLKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKFSQKGFKVKIYSPDKDLLQLVSENVLVINPMNDEVFTKERV
IKKFGVEPQKIPDYLALVGDKVDNVPGIEGVGPKTAINILKKYGSVENILKNWEKFQREFPRAKKEDLELSYKLVKLYTD
LDIELSEEDLKIKRPDLNKLKQKLQELEMKSLLKEVDKIFRQASQRSLF
>Mature_289_residues
MKTLYILDGSSFVYRSFFALPPLSTSKGFPTNAIYGFLRMLFSLIKKERPQYLVVVFDAPAKTKREKIYADYKKQRPKAP
DPLKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKFSQKGFKVKIYSPDKDLLQLVSENVLVINPMNDEVFTKERV
IKKFGVEPQKIPDYLALVGDKVDNVPGIEGVGPKTAINILKKYGSVENILKNWEKFQREFPRAKKEDLELSYKLVKLYTD
LDIELSEEDLKIKRPDLNKLKQKLQELEMKSLLKEVDKIFRQASQRSLF

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain

Homologues:

Organism=Escherichia coli, GI1790294, Length=284, Percent_Identity=40.8450704225352, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI226510970, Length=257, Percent_Identity=30.7392996108949, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EX53_AQUAE (O67550)

Other databases:

- EMBL:   AE000657
- PIR:   D70440
- RefSeq:   NP_214115.1
- ProteinModelPortal:   O67550
- SMR:   O67550
- GeneID:   1193173
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1628
- NMPDR:   fig|224324.1.peg.1130
- HOGENOM:   HBG701384
- OMA:   NESEDLP
- PhylomeDB:   O67550
- ProtClustDB:   CLSK2299775
- BioCyc:   AAEO224324:AQ_1628-MONOMER
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583
- SMART:   SM00475
- SMART:   SM00278
- SMART:   SM00279

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; SSF47807 5_3_exo_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 33323; Mature: 33323

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLYILDGSSFVYRSFFALPPLSTSKGFPTNAIYGFLRMLFSLIKKERPQYLVVVFDAP
CCEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
AKTKREKIYADYKKQRPKAPDPLKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKF
CHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHH
SQKGFKVKIYSPDKDLLQLVSENVLVINPMNDEVFTKERVIKKFGVEPQKIPDYLALVGD
HCCCCEEEEECCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KVDNVPGIEGVGPKTAINILKKYGSVENILKNWEKFQREFPRAKKEDLELSYKLVKLYTD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
LDIELSEEDLKIKRPDLNKLKQKLQELEMKSLLKEVDKIFRQASQRSLF
CCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTLYILDGSSFVYRSFFALPPLSTSKGFPTNAIYGFLRMLFSLIKKERPQYLVVVFDAP
CCEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
AKTKREKIYADYKKQRPKAPDPLKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKF
CHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHH
SQKGFKVKIYSPDKDLLQLVSENVLVINPMNDEVFTKERVIKKFGVEPQKIPDYLALVGD
HCCCCEEEEECCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KVDNVPGIEGVGPKTAINILKKYGSVENILKNWEKFQREFPRAKKEDLELSYKLVKLYTD
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
LDIELSEEDLKIKRPDLNKLKQKLQELEMKSLLKEVDKIFRQASQRSLF
CCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320