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The map label for this gene is dus

Identifier: 15606718

GI number: 15606718

Start: 1124683

End: 1125633

Strand: Direct

Name: dus

Synonym: aq_1598

Alternate gene names: 15606718

Gene position: 1124683-1125633 (Clockwise)

Preceding gene: 15606715

Following gene: 15606730

Centisome position: 72.5

GC content: 45.01

Gene sequence:

>951_bases
ATGAACTTCAAAAAGGGAGAGGTAATTCTCGCACCTATGGCCTCTTACACCCACTCCGCATTCAGGAGACTTTGCAGGAG
ACTGGGAGCGGATAGAACTTACACGGAACTTATAAGTGCGGTCGGTGTCCTCAGAAACGGAATTCCCATGAAGCTCGCTT
ACTTTACAGAAGAAGAAAGACCAATACATATTCAGGTTTTCGGAAGCAATCCGGAAGAAATAGCACAAGCAAGCGTTGTA
ATAGCGAAAGAATTAAAGCCCGACTTTATAGACATAAACTTCGGATGTTCTGTTCCCAAGGTTCTGAGAAACAAAGCTGC
CGGTTACATGCTCCAGTGTCCACCTCTCATGGGAGAAGTGGTAAAGGAGACTGTAGAGGCGTTGAAGCCCTACGGTATTC
CCGTTTCTGCAAAAATCAGACTTGGTTTTGAAAAGGACGAGGTGGAGAGGATAGTAGAGGAACTCCAGAAAGCGGGAGTC
TCTTTAATAGCTATTCACGCGAGAACCGCAAAGCAGGGATTTAGCGGGAAAGCCCTCTGGCACAGGATAAAGGAAGCCAA
AAAGGTTGCAAGCGTACCCATAATAGGGAGTGGAGACGTAAAGAGCTGGAGGGATATCGAGAGAATGTTTGAAGAAACGG
AGTGCGACGGTGTTATGGTGGGGCGTGCAGCCCTTTCAAACCCGTGGATATTTAAAGAGTTCAAAGAAAAAAGGGACATA
GAGGTAGGTCTAAAAGAGAGAATGGACTTTATCCTTGAAGAGCTTTCTATGATGACGGAGTATATGAGCAGGGAAAAAGC
GTGTGCGGAAATAAAATCTCAAATAGTTCAAATACTCAAAGGTGTTCCCAATTCAAGGGAATTGAAGACCTACATAGTGC
ATGCGGAAAACTGCAAGGAACTTGTAAAAAGGATAGAGGAGGCGAAGGAGAGGGAATTACTTTATGCCTAA

Upstream 100 bases:

>100_bases
ATGGCGTACGCTTACTACAAGTACAGAAAGGAACAAAAGCGAAGGGAAGAGATACTCAGAAGGTATTACGAGGACCTTTG
GGAAGAATAAACTAATTACT

Downstream 100 bases:

>100_bases
GGTAGTCTTGGGAGTTACGTAGTCTGCAAGTGCCGCTATACCACCTTTCAGAGCGTAAACGTTGTCAAAGCCCGCACTTC
TGAGGGCAAAGGTTGCTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNFKKGEVILAPMASYTHSAFRRLCRRLGADRTYTELISAVGVLRNGIPMKLAYFTEEERPIHIQVFGSNPEEIAQASVV
IAKELKPDFIDINFGCSVPKVLRNKAAGYMLQCPPLMGEVVKETVEALKPYGIPVSAKIRLGFEKDEVERIVEELQKAGV
SLIAIHARTAKQGFSGKALWHRIKEAKKVASVPIIGSGDVKSWRDIERMFEETECDGVMVGRAALSNPWIFKEFKEKRDI
EVGLKERMDFILEELSMMTEYMSREKACAEIKSQIVQILKGVPNSRELKTYIVHAENCKELVKRIEEAKERELLYA

Sequences:

>Translated_316_residues
MNFKKGEVILAPMASYTHSAFRRLCRRLGADRTYTELISAVGVLRNGIPMKLAYFTEEERPIHIQVFGSNPEEIAQASVV
IAKELKPDFIDINFGCSVPKVLRNKAAGYMLQCPPLMGEVVKETVEALKPYGIPVSAKIRLGFEKDEVERIVEELQKAGV
SLIAIHARTAKQGFSGKALWHRIKEAKKVASVPIIGSGDVKSWRDIERMFEETECDGVMVGRAALSNPWIFKEFKEKRDI
EVGLKERMDFILEELSMMTEYMSREKACAEIKSQIVQILKGVPNSRELKTYIVHAENCKELVKRIEEAKERELLYA
>Mature_316_residues
MNFKKGEVILAPMASYTHSAFRRLCRRLGADRTYTELISAVGVLRNGIPMKLAYFTEEERPIHIQVFGSNPEEIAQASVV
IAKELKPDFIDINFGCSVPKVLRNKAAGYMLQCPPLMGEVVKETVEALKPYGIPVSAKIRLGFEKDEVERIVEELQKAGV
SLIAIHARTAKQGFSGKALWHRIKEAKKVASVPIIGSGDVKSWRDIERMFEETECDGVMVGRAALSNPWIFKEFKEKRDI
EVGLKERMDFILEELSMMTEYMSREKACAEIKSQIVQILKGVPNSRELKTYIVHAENCKELVKRIEEAKERELLYA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family

Homologues:

Organism=Homo sapiens, GI239788483, Length=251, Percent_Identity=29.0836653386454, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI239788462, Length=251, Percent_Identity=29.0836653386454, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=31.0043668122271, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=267, Percent_Identity=30.3370786516854, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=33.6134453781513, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=29.0598290598291, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693211, Length=311, Percent_Identity=26.3665594855305, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=281, Percent_Identity=29.8932384341637, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=26.4069264069264, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=250, Percent_Identity=27.2, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323437, Length=233, Percent_Identity=29.6137339055794, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=285, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=296, Percent_Identity=23.9864864864865, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=30.8016877637131, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=30.8370044052863, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=30.8370044052863, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.82096069869, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUS_AQUAE (O67533)

Other databases:

- EMBL:   AE000657
- PIR:   C70438
- RefSeq:   NP_214098.1
- ProteinModelPortal:   O67533
- SMR:   O67533
- GeneID:   1193156
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1598
- NMPDR:   fig|224324.1.peg.1113
- HOGENOM:   HBG557545
- OMA:   GCSVPKV
- PhylomeDB:   O67533
- BioCyc:   AAEO224324:AQ_1598-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 35808; Mature: 35808

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKKGEVILAPMASYTHSAFRRLCRRLGADRTYTELISAVGVLRNGIPMKLAYFTEEER
CCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
PIHIQVFGSNPEEIAQASVVIAKELKPDFIDINFGCSVPKVLRNKAAGYMLQCPPLMGEV
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCEEECCHHHHHH
VKETVEALKPYGIPVSAKIRLGFEKDEVERIVEELQKAGVSLIAIHARTAKQGFSGKALW
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCHHHHH
HRIKEAKKVASVPIIGSGDVKSWRDIERMFEETECDGVMVGRAALSNPWIFKEFKEKRDI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHCCH
EVGLKERMDFILEELSMMTEYMSREKACAEIKSQIVQILKGVPNSRELKTYIVHAENCKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHH
LVKRIEEAKERELLYA
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFKKGEVILAPMASYTHSAFRRLCRRLGADRTYTELISAVGVLRNGIPMKLAYFTEEER
CCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
PIHIQVFGSNPEEIAQASVVIAKELKPDFIDINFGCSVPKVLRNKAAGYMLQCPPLMGEV
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCEEECCHHHHHH
VKETVEALKPYGIPVSAKIRLGFEKDEVERIVEELQKAGVSLIAIHARTAKQGFSGKALW
HHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCHHHHH
HRIKEAKKVASVPIIGSGDVKSWRDIERMFEETECDGVMVGRAALSNPWIFKEFKEKRDI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECHHHHCCCHHHHHHHHHCCH
EVGLKERMDFILEELSMMTEYMSREKACAEIKSQIVQILKGVPNSRELKTYIVHAENCKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHH
LVKRIEEAKERELLYA
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320