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The map label for this gene is rpoS [H]

Identifier: 15606622

GI number: 15606622

Start: 1036324

End: 1037256

Strand: Direct

Name: rpoS [H]

Synonym: aq_1452

Alternate gene names: 15606622

Gene position: 1036324-1037256 (Clockwise)

Preceding gene: 15606621

Following gene: 15606623

Centisome position: 66.8

GC content: 45.55

Gene sequence:

>933_bases
ATGATACCAGATACAGAGCAAGAACTTATAAATCAGTACTTGAAGAAAATTTCAAAGATACCGCTCCTCACACCGGAAGA
GGAGAAGGAGCTGGCGAGGAGGGCTAAACAGGGGGACAAAGAAGCTCTAAAAAAACTCGTGGAGTCTAACCTCAGGTTCG
TGGTGAGCGTTGCAAAGCAATACATAGGTTACGGTCTTCCTTTTTCCGAATTAATCGCCGCGGGAAACCTCGGTCTTCTG
GAAGCCGCTAAACGCTTTGACCCCGACAGAGGGGTTAAGTTCATCTCTTACGCGGTATGGTGGATAAGACAGGCGATAAT
GCAGGCACTCTCTCAACAAACGGGGGCTGTAAGAATACCCCTGAAACAGTCCCACATAATCAACAAGATTTCACACATAT
ACGGAGAACTTTCAAAGAAACTAGAAAGAGAACCCTCTCCAGAAGAAATAGCTGAAGAGCTAAGCAAACAGATGATAGCG
AAGGAACTGGAAAAGGAACTCGGCAGAGAACCTACCGAAGAGGAAATAGAAGAAAGGTTCAAAAAGGAAGGATACAAAAT
TACCCCCGAAGAAGTTGAGAAGTACCTTCAGGTTTGCAGAGTTCCCCTCTCTCTGGATGCTCCCGTAGGCGAAAACGAAG
ACACCTTCTTCATAGACTTTTTGAGCAAACACGGGACTGCGGAAGTGGAGGAAAAAGTAATACAGGAAGTAATACAGAAG
GAAATAGACGACCTCCTGAGCAAGCTGACGGATAAAGAGAGGAAGGTAATAGAACTCAGGTTCGGCCTTAAGGGAAACGA
ACCCAAGACCTTAAGGGAAATAGGTGAGATACTCGGACTCTCCAGAGAAAGGGTAAGACAGTTGGAAACAAGAGCCCTGA
GGAAATTGAGAAGTCTTGCGATGAAGAGGCACTTAAAAGACTTCCTTAGCTGA

Upstream 100 bases:

>100_bases
AACAGATTAAAGGAAATGGGAAGGTCTAAGGCTCTGCTCCTTGTAAGGAGGGGTGACAGAAATATCTTTATAACTCTGGA
CTTAGAGTGAGGTAGTAGCC

Downstream 100 bases:

>100_bases
TGAAGTGCGTAGTTTACGGTTCAAAAAGTCCGGTTCTTCACCAGGCCCTCGAAGAGGTAAAGAGAAAATTTCTGGAGGAA
AAGATACTCACGGACTTTGA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIPDTEQELINQYLKKISKIPLLTPEEEKELARRAKQGDKEALKKLVESNLRFVVSVAKQYIGYGLPFSELIAAGNLGLL
EAAKRFDPDRGVKFISYAVWWIRQAIMQALSQQTGAVRIPLKQSHIINKISHIYGELSKKLEREPSPEEIAEELSKQMIA
KELEKELGREPTEEEIEERFKKEGYKITPEEVEKYLQVCRVPLSLDAPVGENEDTFFIDFLSKHGTAEVEEKVIQEVIQK
EIDDLLSKLTDKERKVIELRFGLKGNEPKTLREIGEILGLSRERVRQLETRALRKLRSLAMKRHLKDFLS

Sequences:

>Translated_310_residues
MIPDTEQELINQYLKKISKIPLLTPEEEKELARRAKQGDKEALKKLVESNLRFVVSVAKQYIGYGLPFSELIAAGNLGLL
EAAKRFDPDRGVKFISYAVWWIRQAIMQALSQQTGAVRIPLKQSHIINKISHIYGELSKKLEREPSPEEIAEELSKQMIA
KELEKELGREPTEEEIEERFKKEGYKITPEEVEKYLQVCRVPLSLDAPVGENEDTFFIDFLSKHGTAEVEEKVIQEVIQK
EIDDLLSKLTDKERKVIELRFGLKGNEPKTLREIGEILGLSRERVRQLETRALRKLRSLAMKRHLKDFLS
>Mature_310_residues
MIPDTEQELINQYLKKISKIPLLTPEEEKELARRAKQGDKEALKKLVESNLRFVVSVAKQYIGYGLPFSELIAAGNLGLL
EAAKRFDPDRGVKFISYAVWWIRQAIMQALSQQTGAVRIPLKQSHIINKISHIYGELSKKLEREPSPEEIAEELSKQMIA
KELEKELGREPTEEEIEERFKKEGYKITPEEVEKYLQVCRVPLSLDAPVGENEDTFFIDFLSKHGTAEVEEKVIQEVIQK
EIDDLLSKLTDKERKVIELRFGLKGNEPKTLREIGEILGLSRERVRQLETRALRKLRSLAMKRHLKDFLS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=303, Percent_Identity=34.6534653465347, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1789098, Length=294, Percent_Identity=36.3945578231293, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI1789871, Length=292, Percent_Identity=28.4246575342466, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35778; Mature: 35778

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDTEQELINQYLKKISKIPLLTPEEEKELARRAKQGDKEALKKLVESNLRFVVSVAKQ
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YIGYGLPFSELIAAGNLGLLEAAKRFDPDRGVKFISYAVWWIRQAIMQALSQQTGAVRIP
HHCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
LKQSHIINKISHIYGELSKKLEREPSPEEIAEELSKQMIAKELEKELGREPTEEEIEERF
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKEGYKITPEEVEKYLQVCRVPLSLDAPVGENEDTFFIDFLSKHGTAEVEEKVIQEVIQK
HHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHH
EIDDLLSKLTDKERKVIELRFGLKGNEPKTLREIGEILGLSRERVRQLETRALRKLRSLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MKRHLKDFLS
HHHHHHHHHC
>Mature Secondary Structure
MIPDTEQELINQYLKKISKIPLLTPEEEKELARRAKQGDKEALKKLVESNLRFVVSVAKQ
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YIGYGLPFSELIAAGNLGLLEAAKRFDPDRGVKFISYAVWWIRQAIMQALSQQTGAVRIP
HHCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECC
LKQSHIINKISHIYGELSKKLEREPSPEEIAEELSKQMIAKELEKELGREPTEEEIEERF
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KKEGYKITPEEVEKYLQVCRVPLSLDAPVGENEDTFFIDFLSKHGTAEVEEKVIQEVIQK
HHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHH
EIDDLLSKLTDKERKVIELRFGLKGNEPKTLREIGEILGLSRERVRQLETRALRKLRSLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MKRHLKDFLS
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]