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The map label for this gene is nuoB

Identifier: 15606518

GI number: 15606518

Start: 928798

End: 929337

Strand: Direct

Name: nuoB

Synonym: aq_1312

Alternate gene names: 15606518

Gene position: 928798-929337 (Clockwise)

Preceding gene: 15606517

Following gene: 15606519

Centisome position: 59.87

GC content: 48.52

Gene sequence:

>540_bases
ATGGTTGCAATAAATTCTAACGGTTTTGTAACAACTACCGTAGAAGAACTCCTCAGATGGGGAAGGAGGAATTCCCTCTG
GCCCGTTACGATAGGGCTCGCTTGCTGTGCTATAGAGATGATGCACACCGCGGCTTCAAGGTTTGACCTTGACAGACTGG
GAGTGATATTCAGGGCTTCTCCGAGACAGGCTGACGTTCTCATAGTTGCGGGAACTGTTGTGAACAAGGTAGCACCAATG
CTGAAACTCATATGGGATCAAATGCCGGATCCCAAGTGGTGTATATCCATGGGCGGATGTGCGTCCGCGGGAGGACCTTT
CCCGACTTATTCCACACTTCAGGGGGTTGACAGGATAATACCCGTTGACGTTTACATACCCGGATGCCCTCCCACACCCC
AGGGACTCATATACGGAATACTCCAACTCCAGAGAAAGATCAAAGAGCAAGGAATAACAAAGTACGATAAACTCTTCGCA
GACTTCAACAGGGAAATAGAGAAGGAAGGTATTTTCGTTCCCAGAGAACTAAAGGTGTGA

Upstream 100 bases:

>100_bases
ATTAATTGAAGTCATTGTCTTTGTGGGAATACTCACATTAGGTTTTATATACGCTTGGCGTAAGGGTGCGTTAAACTGGG
AGATGTAGGAGGTAAGTGCC

Downstream 100 bases:

>100_bases
GGATAACTTATGAAATGGGTCAATAAGGGAACCGTTGAGAGGGTAAAGCAGGAATTTAAGGACGAAGTAAAGTACTACGA
GACAAAACACACTAAAGGTT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MVAINSNGFVTTTVEELLRWGRRNSLWPVTIGLACCAIEMMHTAASRFDLDRLGVIFRASPRQADVLIVAGTVVNKVAPM
LKLIWDQMPDPKWCISMGGCASAGGPFPTYSTLQGVDRIIPVDVYIPGCPPTPQGLIYGILQLQRKIKEQGITKYDKLFA
DFNREIEKEGIFVPRELKV

Sequences:

>Translated_179_residues
MVAINSNGFVTTTVEELLRWGRRNSLWPVTIGLACCAIEMMHTAASRFDLDRLGVIFRASPRQADVLIVAGTVVNKVAPM
LKLIWDQMPDPKWCISMGGCASAGGPFPTYSTLQGVDRIIPVDVYIPGCPPTPQGLIYGILQLQRKIKEQGITKYDKLFA
DFNREIEKEGIFVPRELKV
>Mature_179_residues
MVAINSNGFVTTTVEELLRWGRRNSLWPVTIGLACCAIEMMHTAASRFDLDRLGVIFRASPRQADVLIVAGTVVNKVAPM
LKLIWDQMPDPKWCISMGGCASAGGPFPTYSTLQGVDRIIPVDVYIPGCPPTPQGLIYGILQLQRKIKEQGITKYDKLFA
DFNREIEKEGIFVPRELKV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=61.5384615384615, Blast_Score=207, Evalue=4e-54,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=47.2972972972973, Blast_Score=161, Evalue=2e-41,
Organism=Escherichia coli, GI1789074, Length=137, Percent_Identity=41.6058394160584, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=40.650406504065, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=59.4405594405594, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI18859983, Length=137, Percent_Identity=63.5036496350365, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24642371, Length=137, Percent_Identity=63.5036496350365, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=62.6760563380282, Blast_Score=204, Evalue=3e-53,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_AQUAE (O67334)

Other databases:

- EMBL:   AE000657
- PIR:   C70413
- RefSeq:   NP_213898.1
- ProteinModelPortal:   O67334
- SMR:   O67334
- GeneID:   1192772
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1312
- NMPDR:   fig|224324.1.peg.913
- HOGENOM:   HBG553221
- OMA:   HYDNERF
- PhylomeDB:   O67334
- ProtClustDB:   PRK06411
- BioCyc:   AAEO224324:AQ_1312-MONOMER
- BRENDA:   1.6.99.5
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19881; Mature: 19881

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAINSNGFVTTTVEELLRWGRRNSLWPVTIGLACCAIEMMHTAASRFDLDRLGVIFRAS
CEEECCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEECC
PRQADVLIVAGTVVNKVAPMLKLIWDQMPDPKWCISMGGCASAGGPFPTYSTLQGVDRII
CCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHEE
PVDVYIPGCPPTPQGLIYGILQLQRKIKEQGITKYDKLFADFNREIEKEGIFVPRELKV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MVAINSNGFVTTTVEELLRWGRRNSLWPVTIGLACCAIEMMHTAASRFDLDRLGVIFRAS
CEEECCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEECC
PRQADVLIVAGTVVNKVAPMLKLIWDQMPDPKWCISMGGCASAGGPFPTYSTLQGVDRII
CCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCHHHHHHHHHEE
PVDVYIPGCPPTPQGLIYGILQLQRKIKEQGITKYDKLFADFNREIEKEGIFVPRELKV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320