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The map label for this gene is gltX

Identifier: 15606455

GI number: 15606455

Start: 859084

End: 860505

Strand: Direct

Name: gltX

Synonym: aq_1221

Alternate gene names: 15606455

Gene position: 859084-860505 (Clockwise)

Preceding gene: 15606452

Following gene: 15606456

Centisome position: 55.38

GC content: 46.91

Gene sequence:

>1422_bases
ATGAGTAAAGTGAAAACGAGGTTCGCACCCAGTCCAACCGGATACCTACACCTCGGAAACGCAAGAACAGCCATATTCAG
TTATCTGTTTGCAAGGCACAACAACGGAGGTTTTGTACTTCGTATAGAGGACACCGATCCAGAGCGTTCAAAGAAAGAGT
ATGAAGAAATGCTGATAGAGGATTTAAAGTGGCTCGGTATAGATTGGGACGAATTCTACAGACAGTCCGAGAGGTTTGAC
ATATACAGGGAGTACGTGAACAAGCTCCTTGAGAGCGGTCACGCTTACCCGTGTTTTTGCACGCCGGAAGAACTTGAAAA
AGAAAGAGAAGAGGCTAGAAAGAAGGGAATTCCTTACAGGTATTCTGGAAAGTGCAGACACCTGACACCAGAAGAGGTGG
AGAAGTTCAAGAAGGAGGGAAAACCCTTCGCCATAAGGTTTAAAGTTCCCGAAAACAGAACCGTGGTGTTTGAAGACCTA
ATAAAGGGTCACATAGCCATTAACACGGATGACTTCGGGGACTTTGTAATTGTCAGGAGCGATGGCTCACCCACTTACAA
CTTCGTCGTTGTTGTTGACGACGCCCTTATGGGAATTACCCACGTGATAAGAGGAGAGGACCACATACCCAACACTCCCA
AGCAAATACTCATATACGAAGCTCTCGGGTTTCCCGTTCCCAAGTTCGCACACCTTCCCGTTATACTGGGAGAGGACAGG
AGTAAACTCTCAAAGAGGCACGGAGCTGTTTCGGTAAGGGCTTACAGGGAAGAAGGCTATATGCCGGAAGCCCTCTTCAA
TTACCTCTGTCTCTTAGGGTGGTCCCCTCCAGAAGAGGGAAGGGAGATATTCTCAAAGGAAGAACTGATAAAGATTTTTG
ACCTCAAAGACGTAAACGACTCCCCGGCGGTCTTCAACAAGGAAAAACTAAAGTGGATGAACGGAGTGTACATAAGGGAG
GTGCTTCCCTTAGACGTTCTCCTTGAAAGGGCTATTCCCTTCTTGGAAAAAGCGGGCTACGACACGAGCGACAGGGAGTA
TATAAAGAAAGTTCTCGAATACACGCGCGACTCCTTTGATACATTGAGCGAAATGGTAGACAGGCTCAGACCCTTCTTCG
TGGACGAGTTTGAAATACCAGAAGAGCTCTGGAGCTTCCTAGACGACGAAAAGGCTTATCAGGTTCTCTCAGCTTTCTTG
GAAAAAATCAGGGAGAAAAAACCCGAAACTCCCCAGGAAGTAAAGAAGCTCGCAAAGGAAATACAAAAAGCCCTGAAAGT
AAAACCACCGCAGGTATGGAAACCCCTCAGGATAGCCCTCACGGGTGAACTCGAAGGTGTGGGCATAGACATACTCATAG
CCGTTCTTCCTAAGGAAAAAATAGAAAAGAGGATACTGAGGGTTCTGGAAAAGCTCTCGTGA

Upstream 100 bases:

>100_bases
AAGTACAGGCTCCTTGACCTTTTCTTCAGGGTATATTAATTGAAGCCTTACCATGTTCATGATAAGTTTTATTGTAATTC
AAAGGTTAAAATAAACTCTC

Downstream 100 bases:

>100_bases
TTTTCCTCTCCTTTAAAAAATCTTTTAAGAACATAACTATTGAAGGAAAGTTTACCTTTGAGAAGGGATTTAACGTAATT
TTAGGGCCTTCGGGAGCAGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSKVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIEDLKWLGIDWDEFYRQSERFD
IYREYVNKLLESGHAYPCFCTPEELEKEREEARKKGIPYRYSGKCRHLTPEEVEKFKKEGKPFAIRFKVPENRTVVFEDL
IKGHIAINTDDFGDFVIVRSDGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGFPVPKFAHLPVILGEDR
SKLSKRHGAVSVRAYREEGYMPEALFNYLCLLGWSPPEEGREIFSKEELIKIFDLKDVNDSPAVFNKEKLKWMNGVYIRE
VLPLDVLLERAIPFLEKAGYDTSDREYIKKVLEYTRDSFDTLSEMVDRLRPFFVDEFEIPEELWSFLDDEKAYQVLSAFL
EKIREKKPETPQEVKKLAKEIQKALKVKPPQVWKPLRIALTGELEGVGIDILIAVLPKEKIEKRILRVLEKLS

Sequences:

>Translated_473_residues
MSKVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIEDLKWLGIDWDEFYRQSERFD
IYREYVNKLLESGHAYPCFCTPEELEKEREEARKKGIPYRYSGKCRHLTPEEVEKFKKEGKPFAIRFKVPENRTVVFEDL
IKGHIAINTDDFGDFVIVRSDGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGFPVPKFAHLPVILGEDR
SKLSKRHGAVSVRAYREEGYMPEALFNYLCLLGWSPPEEGREIFSKEELIKIFDLKDVNDSPAVFNKEKLKWMNGVYIRE
VLPLDVLLERAIPFLEKAGYDTSDREYIKKVLEYTRDSFDTLSEMVDRLRPFFVDEFEIPEELWSFLDDEKAYQVLSAFL
EKIREKKPETPQEVKKLAKEIQKALKVKPPQVWKPLRIALTGELEGVGIDILIAVLPKEKIEKRILRVLEKLS
>Mature_472_residues
SKVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIEDLKWLGIDWDEFYRQSERFDI
YREYVNKLLESGHAYPCFCTPEELEKEREEARKKGIPYRYSGKCRHLTPEEVEKFKKEGKPFAIRFKVPENRTVVFEDLI
KGHIAINTDDFGDFVIVRSDGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGFPVPKFAHLPVILGEDRS
KLSKRHGAVSVRAYREEGYMPEALFNYLCLLGWSPPEEGREIFSKEELIKIFDLKDVNDSPAVFNKEKLKWMNGVYIREV
LPLDVLLERAIPFLEKAGYDTSDREYIKKVLEYTRDSFDTLSEMVDRLRPFFVDEFEIPEELWSFLDDEKAYQVLSAFLE
KIREKKPETPQEVKKLAKEIQKALKVKPPQVWKPLRIALTGELEGVGIDILIAVLPKEKIEKRILRVLEKLS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=502, Percent_Identity=34.6613545816733, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI62241042, Length=231, Percent_Identity=28.1385281385281, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI4826960, Length=105, Percent_Identity=34.2857142857143, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=43.4968017057569, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI226510925, Length=249, Percent_Identity=37.7510040160643, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=210, Percent_Identity=30.4761904761905, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=31.8181818181818, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17511007, Length=245, Percent_Identity=25.7142857142857, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543332, Length=104, Percent_Identity=31.7307692307692, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=38.1632653061225, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6321192, Length=305, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324742, Length=125, Percent_Identity=35.2, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=37, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24649466, Length=221, Percent_Identity=27.1493212669683, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17864488, Length=105, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_AQUAE (O67271)

Other databases:

- EMBL:   AE000657
- PIR:   D70405
- RefSeq:   NP_213835.1
- ProteinModelPortal:   O67271
- SMR:   O67271
- GeneID:   1192617
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1221
- NMPDR:   fig|224324.1.peg.850
- HOGENOM:   HBG628189
- OMA:   DEASWTK
- PhylomeDB:   O67271
- ProtClustDB:   PRK01406
- BioCyc:   AAEO224324:AQ_1221-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.8.70
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55122; Mature: 54991

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIE
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
DLKWLGIDWDEFYRQSERFDIYREYVNKLLESGHAYPCFCTPEELEKEREEARKKGIPYR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCCEE
YSGKCRHLTPEEVEKFKKEGKPFAIRFKVPENRTVVFEDLIKGHIAINTDDFGDFVIVRS
ECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHCCCEEEECCCCCCEEEEEC
DGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGFPVPKFAHLPVILGEDR
CCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCCCCCCEEECCCH
SKLSKRHGAVSVRAYREEGYMPEALFNYLCLLGWSPPEEGREIFSKEELIKIFDLKDVND
HHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHCCCCCCCC
SPAVFNKEKLKWMNGVYIREVLPLDVLLERAIPFLEKAGYDTSDREYIKKVLEYTRDSFD
CCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
TLSEMVDRLRPFFVDEFEIPEELWSFLDDEKAYQVLSAFLEKIREKKPETPQEVKKLAKE
HHHHHHHHHCCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IQKALKVKPPQVWKPLRIALTGELEGVGIDILIAVLPKEKIEKRILRVLEKLS
HHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVKTRFAPSPTGYLHLGNARTAIFSYLFARHNNGGFVLRIEDTDPERSKKEYEEMLIE
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHH
DLKWLGIDWDEFYRQSERFDIYREYVNKLLESGHAYPCFCTPEELEKEREEARKKGIPYR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCCEE
YSGKCRHLTPEEVEKFKKEGKPFAIRFKVPENRTVVFEDLIKGHIAINTDDFGDFVIVRS
ECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHCCCEEEECCCCCCEEEEEC
DGSPTYNFVVVVDDALMGITHVIRGEDHIPNTPKQILIYEALGFPVPKFAHLPVILGEDR
CCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCCCCCCEEECCCH
SKLSKRHGAVSVRAYREEGYMPEALFNYLCLLGWSPPEEGREIFSKEELIKIFDLKDVND
HHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHCCCCCCCC
SPAVFNKEKLKWMNGVYIREVLPLDVLLERAIPFLEKAGYDTSDREYIKKVLEYTRDSFD
CCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHH
TLSEMVDRLRPFFVDEFEIPEELWSFLDDEKAYQVLSAFLEKIREKKPETPQEVKKLAKE
HHHHHHHHHCCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IQKALKVKPPQVWKPLRIALTGELEGVGIDILIAVLPKEKIEKRILRVLEKLS
HHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320