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The map label for this gene is purF [H]

Identifier: 15606420

GI number: 15606420

Start: 825994

End: 827391

Strand: Direct

Name: purF [H]

Synonym: aq_1175

Alternate gene names: 15606420

Gene position: 825994-827391 (Clockwise)

Preceding gene: 15606419

Following gene: 15606421

Centisome position: 53.24

GC content: 44.92

Gene sequence:

>1398_bases
ATGTGCGGAGTTGCAGGAGTCTTTAATTCCCCTTACGCAGCGGAATATGCCCTTTTAATGATTTACTCACTCCAGCACAG
GGGACAGGAAAGTGTAGGGATAAGTTCCTCTGATGAAAAGGAAATTTACACCGTTAAAAAACCCGGAAGGGTTTTAGAAG
CGATAAAAAAAGAAGACCTTGAAAAATTAAAAGGAAGTATAGCGATAGCACACGTCAGGTACTCAACAGCGGGAAACGCA
GGAGGCTTTAACGCCCAGCCTCTTGTGAGGGAAACACCTCTCGGAAAAGTTGCGGTAGTACACAACGGAAACCTCGTAAA
CTTCCTAACTTTGAGAAAGGAGCTTGAAGATAAAGGTATAAAGTTCAATTACAACTCTGACAGCGAACTCTTTTTAGCAC
TTCTTGAGGAAGGAAGGACCTATATACCTGAAGGTGTAACGCTTCATCCGGAGGACAAGGATTTCCTTCCCTACATCTTT
TACGCCCTTTCTAGAGTTAAAGGTGCCTACAGTTTGATATACCTCTTTAGGGACAAGCTCGTAGTTGCGAGGGATCCATA
CGGTTTCAGACCCTTAGCCTTGGGAAGGAAGAAAAACACCATTCTGGTAGCTTCGGAGAGCTGTGCCTTTGACATACTTG
AAGCTGAATTCTGGAGAGAAGTAAAACCGGGTGAAGTTCTCGTAATAGACAAAAAAGGAAAGAGGAGCTACAGGATATTC
AGTTCACCCAGAAAAGCTATGTGCATATTTGAGTTTGTGTACTTCTCAAGACCGGACAGCTACATATTCGGCGACTGGGT
ATACAACGTGAGAAAAGAAATGGGAAGACAGCTTGCGAGAGAAGATAACATAGAAGCGGACGTTGTCATTCCCGTTCCCG
ATTCTGGAGTCGTTCCGGCACTCGGATACGCGGAGGAAAAGGGACTGCCTTTTGAGTACGGGCTTATAAGAAACCACTAC
GTGGGAAGGAGCTTTATAGAACCAACACCCGAACTCAGAAACTTAAGAGTTTTAATGAAGCTCTCTCCCAACAGAGCCGT
TCTGCAAGGGAAGAGAGTTATAGTTATAGATGACTCCCTCGTGAGAGGAACTACCTCAAAGAGGATAGTCAGCATGCTCA
GGAGGGCAGGTGCTAAGGAAATACACATGAGGATAGCCTCTCCGCCCGTCGTGGGACCCTGTTACTACGGCATAGACACG
CCCACAAAAGAGGAGTTAATAGCAAACAAAATGCCGATAGAGGATATAGCCAAGTTCATAGGTGTAGATTCCCTGAAGTA
CCTTTCTTTAGAAGGCTTGAGAAAGTGTGTTGATAAGCCTGAAGATTTCTGCGACGCATGCTTTAGCCTGAATTATCCCG
TAGAAGTTGAAGATAATGTCCCCTCAAAGATACGCTAA

Upstream 100 bases:

>100_bases
AGCTAAAAAGCTGGGTATGATAAGGGACGGTGTAATTCCCTTTGAGGCGAAAGTGCTCAGGTGTGGTTACTGAAAAAGGT
GTATATTTAAACTTGTTAAG

Downstream 100 bases:

>100_bases
AAATTTGACAAATCTAAACTAAAGAAATATCATAAAAATTGTTAAAACTCACAGGGAGGTGGAAAGATGGCTACAAAAGC
TATACCAAAAGAACAGTGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDLEKLKGSIAIAHVRYSTAGNA
GGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKGIKFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIF
YALSRVKGAYSLIYLFRDKLVVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKGKRSYRIF
SSPRKAMCIFEFVYFSRPDSYIFGDWVYNVRKEMGRQLAREDNIEADVVIPVPDSGVVPALGYAEEKGLPFEYGLIRNHY
VGRSFIEPTPELRNLRVLMKLSPNRAVLQGKRVIVIDDSLVRGTTSKRIVSMLRRAGAKEIHMRIASPPVVGPCYYGIDT
PTKEELIANKMPIEDIAKFIGVDSLKYLSLEGLRKCVDKPEDFCDACFSLNYPVEVEDNVPSKIR

Sequences:

>Translated_465_residues
MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDLEKLKGSIAIAHVRYSTAGNA
GGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKGIKFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIF
YALSRVKGAYSLIYLFRDKLVVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKGKRSYRIF
SSPRKAMCIFEFVYFSRPDSYIFGDWVYNVRKEMGRQLAREDNIEADVVIPVPDSGVVPALGYAEEKGLPFEYGLIRNHY
VGRSFIEPTPELRNLRVLMKLSPNRAVLQGKRVIVIDDSLVRGTTSKRIVSMLRRAGAKEIHMRIASPPVVGPCYYGIDT
PTKEELIANKMPIEDIAKFIGVDSLKYLSLEGLRKCVDKPEDFCDACFSLNYPVEVEDNVPSKIR
>Mature_465_residues
MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDLEKLKGSIAIAHVRYSTAGNA
GGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKGIKFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIF
YALSRVKGAYSLIYLFRDKLVVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKGKRSYRIF
SSPRKAMCIFEFVYFSRPDSYIFGDWVYNVRKEMGRQLAREDNIEADVVIPVPDSGVVPALGYAEEKGLPFEYGLIRNHY
VGRSFIEPTPELRNLRVLMKLSPNRAVLQGKRVIVIDDSLVRGTTSKRIVSMLRRAGAKEIHMRIASPPVVGPCYYGIDT
PTKEELIANKMPIEDIAKFIGVDSLKYLSLEGLRKCVDKPEDFCDACFSLNYPVEVEDNVPSKIR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=39.3063583815029, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1788651, Length=456, Percent_Identity=38.1578947368421, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790167, Length=249, Percent_Identity=28.1124497991968, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=39.9168399168399, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17539970, Length=230, Percent_Identity=28.695652173913, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=493, Percent_Identity=37.525354969574, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24659598, Length=468, Percent_Identity=41.8803418803419, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=42.1166306695464, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=40.3225806451613, Blast_Score=347, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52390; Mature: 52390

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
EKLKGSIAIAHVRYSTAGNAGGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKGI
HHHCCCEEEEEEEEECCCCCCCCCCCCCCEECCCCEEEEEECCCEEEHHHHHHHHHHCCE
KFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIFYALSRVKGAYSLIYLFRDKL
EEEECCCHHHHHHHHHCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCE
VVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKGKRSYRIF
EEEECCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEE
SSPRKAMCIFEFVYFSRPDSYIFGDWVYNVRKEMGRQLAREDNIEADVVIPVPDSGVVPA
CCCCHHHHHEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
LGYAEEKGLPFEYGLIRNHYVGRSFIEPTPELRNLRVLMKLSPNRAVLQGKRVIVIDDSL
CCCHHCCCCCEEECHHHHCCCCCHHCCCCCCHHCEEEEEEECCCHHHCCCCEEEEECCCH
VRGTTSKRIVSMLRRAGAKEIHMRIASPPVVGPCYYGIDTPTKEELIANKMPIEDIAKFI
HCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
GVDSLKYLSLEGLRKCVDKPEDFCDACFSLNYPVEVEDNVPSKIR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MCGVAGVFNSPYAAEYALLMIYSLQHRGQESVGISSSDEKEIYTVKKPGRVLEAIKKEDL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
EKLKGSIAIAHVRYSTAGNAGGFNAQPLVRETPLGKVAVVHNGNLVNFLTLRKELEDKGI
HHHCCCEEEEEEEEECCCCCCCCCCCCCCEECCCCEEEEEECCCEEEHHHHHHHHHHCCE
KFNYNSDSELFLALLEEGRTYIPEGVTLHPEDKDFLPYIFYALSRVKGAYSLIYLFRDKL
EEEECCCHHHHHHHHHCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCE
VVARDPYGFRPLALGRKKNTILVASESCAFDILEAEFWREVKPGEVLVIDKKGKRSYRIF
EEEECCCCCCCEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEE
SSPRKAMCIFEFVYFSRPDSYIFGDWVYNVRKEMGRQLAREDNIEADVVIPVPDSGVVPA
CCCCHHHHHEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
LGYAEEKGLPFEYGLIRNHYVGRSFIEPTPELRNLRVLMKLSPNRAVLQGKRVIVIDDSL
CCCHHCCCCCEEECHHHHCCCCCHHCCCCCCHHCEEEEEEECCCHHHCCCCEEEEECCCH
VRGTTSKRIVSMLRRAGAKEIHMRIASPPVVGPCYYGIDTPTKEELIANKMPIEDIAKFI
HCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
GVDSLKYLSLEGLRKCVDKPEDFCDACFSLNYPVEVEDNVPSKIR
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]