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The map label for this gene is carB1

Identifier: 15606417

GI number: 15606417

Start: 823159

End: 824772

Strand: Direct

Name: carB1

Synonym: aq_1172

Alternate gene names: 15606417

Gene position: 823159-824772 (Clockwise)

Preceding gene: 15606416

Following gene: 15606418

Centisome position: 53.06

GC content: 43.8

Gene sequence:

>1614_bases
ATGTCTAAAAAGGTTGTAATACTCGGAAGCGGACCCAACAGAATAGGACAAGGTATAGAGTTCGATTACGCCTGTGTTCA
CGCCGTTTTTTCTCTTCAAGAAGAAGGTTATTACGCCGTAATGGTTAACTGCAATCCCGAAACCGTTTCTACCGACTACG
ACACAGCCGATAAACTCTACTTTGAGCCTATTGTCTTTGAACACGTAATGGACATAATAGAGCGAGAAAAACCCGAAGGC
GTTATACTCCAGTTTGGCGGACAAACCCCTCTGAAGCTAGCACTCCCGCTTCAAAAAAACGGAGTAAAAATCTTAGGAAC
TAAACCCGAAAGCATAGACAAAGCTGAGGACAGGGAACTTTTCAGGGAATTAATAATAGAGCTGGGGCTCAAGCAACCAC
CAAGCGGTACTGCAAGAACAAAAGAAGAAGCCCTAAAAATAGCAAAGGAAATAGGCTTCCCCGTTCTTGTGAGACCCTCT
TACGTACTCGGCGGAAGGGCTATGCGGATAGTCTACGACGAGGAAGAATTGAAGGAATACCTCGAAGAGGCTGTAAGCGT
AAGTCATGAGAGACCCGTATTAATAGACAAATTCCTTGACAACAGTATAGAACTGGACGTTGACGCGGTATCTGACGGAA
AAGACGTTTTAATCGGAGCGGTTATGGAGCACATAGAAGAGGCGGGAGTTCACTCCGGAGACAGCGCTACTTCAATACCC
CCCTACTCCCTTTCCAAGGAAATTGTAGAAGAAGTAAAGGAACAGACGAGAAAACTCGCCGTAGCCCTTGAGGTAAAGGG
ACTCATAAACGTTCAGTACGCGGTTCAAAACAACGAGGTTTACGTCCTTGAGGTAAACCCGCGGGCTTCTAGAACGGTTC
CCTTCGTCAGCAAAAGTATAGGTTATCCCCTTGCAAAAATAGCAACTAAAGTTGCAATTGGGAAAAGCTTGAGGGAAATA
CTACCGGAAGTCTTTGAAAGACTTGAGAAAGGAGAGGCACACTTTGCCTCGGATTTCCTCCCTAAAGAAAAAAAGATATT
CTCTGTAAAGGAAGTCGTATTTCCCTGGAAACGTTTTCCGGAAGTTGACCCGATTTTAGGGCCGGAAATGAAGAGTACGG
GGGAGGTTATGGGGATAGATAAAGAGTTCGGACTCGCCTACTACAAGGCTCAGCTTTCAGCTGGCTACAGACTTCCAGAA
AAGGGAAACTTATTTATAAGCGTTGCGGACAGGGACAAACCCAAAATCCTTGAACTTGCAAAAGAATTTGAGAAACTCGG
TTTTGGTATATATGCAACTTCCGGTACTTACAAATTTCTAAAGGAGCACGGTGTAAACGCCAAAAGGGTTTTGAAGGTTT
CAGAAGGCAGACCTAACGTTGTTGACATGATAATAAACGGAGAAATTCATCTGGTAATAAATACTCCCTCCGGAAAGCGT
GAAAAGAGCGACGCTTATTACATAAGGAGGGCATGCGTTCAGTTTAACGTTCCTTACTACACTACGATGCGTGCGGGATA
CGCAGTTCTTGAAGCAATAAAAAGCATAAAGAAATTAAAAGAAGAAGGAAAAGGCTTAAGTGTTCATTCCCTTCAAGAGA
TTTATAATATTTAG

Upstream 100 bases:

>100_bases
CATTAAAGAAATAAAGGAATTTGTAACCTTTGCAATAGAACAATTTATGGAAGAAATAGACAGCGTGGAGTACGGAGGAG
AATAAGTATAATCTTTCTTA

Downstream 100 bases:

>100_bases
TTAGTAAATCCTAACAGGAGGTGGAAGGATGGGCGACGTAATGATAGCTGTTATAACTATCGTAGTTTTACTCGCGTTAA
TACCCGTATTCTCAAAGCTT

Product: carbamoyl-phosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSKKVVILGSGPNRIGQGIEFDYACVHAVFSLQEEGYYAVMVNCNPETVSTDYDTADKLYFEPIVFEHVMDIIEREKPEG
VILQFGGQTPLKLALPLQKNGVKILGTKPESIDKAEDRELFRELIIELGLKQPPSGTARTKEEALKIAKEIGFPVLVRPS
YVLGGRAMRIVYDEEELKEYLEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIEEAGVHSGDSATSIP
PYSLSKEIVEEVKEQTRKLAVALEVKGLINVQYAVQNNEVYVLEVNPRASRTVPFVSKSIGYPLAKIATKVAIGKSLREI
LPEVFERLEKGEAHFASDFLPKEKKIFSVKEVVFPWKRFPEVDPILGPEMKSTGEVMGIDKEFGLAYYKAQLSAGYRLPE
KGNLFISVADRDKPKILELAKEFEKLGFGIYATSGTYKFLKEHGVNAKRVLKVSEGRPNVVDMIINGEIHLVINTPSGKR
EKSDAYYIRRACVQFNVPYYTTMRAGYAVLEAIKSIKKLKEEGKGLSVHSLQEIYNI

Sequences:

>Translated_537_residues
MSKKVVILGSGPNRIGQGIEFDYACVHAVFSLQEEGYYAVMVNCNPETVSTDYDTADKLYFEPIVFEHVMDIIEREKPEG
VILQFGGQTPLKLALPLQKNGVKILGTKPESIDKAEDRELFRELIIELGLKQPPSGTARTKEEALKIAKEIGFPVLVRPS
YVLGGRAMRIVYDEEELKEYLEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIEEAGVHSGDSATSIP
PYSLSKEIVEEVKEQTRKLAVALEVKGLINVQYAVQNNEVYVLEVNPRASRTVPFVSKSIGYPLAKIATKVAIGKSLREI
LPEVFERLEKGEAHFASDFLPKEKKIFSVKEVVFPWKRFPEVDPILGPEMKSTGEVMGIDKEFGLAYYKAQLSAGYRLPE
KGNLFISVADRDKPKILELAKEFEKLGFGIYATSGTYKFLKEHGVNAKRVLKVSEGRPNVVDMIINGEIHLVINTPSGKR
EKSDAYYIRRACVQFNVPYYTTMRAGYAVLEAIKSIKKLKEEGKGLSVHSLQEIYNI
>Mature_536_residues
SKKVVILGSGPNRIGQGIEFDYACVHAVFSLQEEGYYAVMVNCNPETVSTDYDTADKLYFEPIVFEHVMDIIEREKPEGV
ILQFGGQTPLKLALPLQKNGVKILGTKPESIDKAEDRELFRELIIELGLKQPPSGTARTKEEALKIAKEIGFPVLVRPSY
VLGGRAMRIVYDEEELKEYLEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIEEAGVHSGDSATSIPP
YSLSKEIVEEVKEQTRKLAVALEVKGLINVQYAVQNNEVYVLEVNPRASRTVPFVSKSIGYPLAKIATKVAIGKSLREIL
PEVFERLEKGEAHFASDFLPKEKKIFSVKEVVFPWKRFPEVDPILGPEMKSTGEVMGIDKEFGLAYYKAQLSAGYRLPEK
GNLFISVADRDKPKILELAKEFEKLGFGIYATSGTYKFLKEHGVNAKRVLKVSEGRPNVVDMIINGEIHLVINTPSGKRE
KSDAYYIRRACVQFNVPYYTTMRAGYAVLEAIKSIKKLKEEGKGLSVHSLQEIYNI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI170295797, Length=522, Percent_Identity=40.4214559386973, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI21361331, Length=522, Percent_Identity=40.4214559386973, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI169790915, Length=522, Percent_Identity=40.4214559386973, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI18105007, Length=530, Percent_Identity=37.3584905660377, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI295821183, Length=266, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI65506442, Length=266, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI189095269, Length=191, Percent_Identity=28.7958115183246, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1786216, Length=513, Percent_Identity=54.1910331384016, Blast_Score=577, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI193204318, Length=537, Percent_Identity=38.7337057728119, Blast_Score=357, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6322331, Length=523, Percent_Identity=39.961759082218, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6322569, Length=539, Percent_Identity=35.9925788497217, Blast_Score=313, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24642586, Length=526, Percent_Identity=37.8326996197719, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI45555749, Length=450, Percent_Identity=40.4444444444444, Blast_Score=330, Evalue=2e-90,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB2_AQUAE (O67233)

Other databases:

- EMBL:   AE000657
- PIR:   F70400
- RefSeq:   NP_213797.1
- ProteinModelPortal:   O67233
- GeneID:   1192579
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1172
- NMPDR:   fig|224324.1.peg.812
- HOGENOM:   HBG405439
- OMA:   CVQAVFA
- ProtClustDB:   CLSK382519
- BioCyc:   AAEO224324:AQ_1172-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_B
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.1380
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098
- SMART:   SM00851

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02142 MGS; SSF52335 MGS-like_dom; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 60038; Mature: 59907

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVVILGSGPNRIGQGIEFDYACVHAVFSLQEEGYYAVMVNCNPETVSTDYDTADKLY
CCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHE
FEPIVFEHVMDIIEREKPEGVILQFGGQTPLKLALPLQKNGVKILGTKPESIDKAEDREL
EHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHH
FRELIIELGLKQPPSGTARTKEEALKIAKEIGFPVLVRPSYVLGGRAMRIVYDEEELKEY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEECCEEEEEEECHHHHHHH
LEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIEEAGVHSGDSATSIP
HHHHHCCCCCCCEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYSLSKEIVEEVKEQTRKLAVALEVKGLINVQYAVQNNEVYVLEVNPRASRTVPFVSKSI
CCHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHH
GYPLAKIATKVAIGKSLREILPEVFERLEKGEAHFASDFLPKEKKIFSVKEVVFPWKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCC
EVDPILGPEMKSTGEVMGIDKEFGLAYYKAQLSAGYRLPEKGNLFISVADRDKPKILELA
CCCCCCCCCCCCCCCEEECCHHHCHHHEEHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
KEFEKLGFGIYATSGTYKFLKEHGVNAKRVLKVSEGRPNVVDMIINGEIHLVINTPSGKR
HHHHHCCCEEEECCCHHHHHHHCCCCHHHEEEECCCCCCEEEEEECCEEEEEEECCCCCC
EKSDAYYIRRACVQFNVPYYTTMRAGYAVLEAIKSIKKLKEEGKGLSVHSLQEIYNI
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SKKVVILGSGPNRIGQGIEFDYACVHAVFSLQEEGYYAVMVNCNPETVSTDYDTADKLY
CCEEEEECCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHE
FEPIVFEHVMDIIEREKPEGVILQFGGQTPLKLALPLQKNGVKILGTKPESIDKAEDREL
EHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHH
FRELIIELGLKQPPSGTARTKEEALKIAKEIGFPVLVRPSYVLGGRAMRIVYDEEELKEY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEECCEEEEEEECHHHHHHH
LEEAVSVSHERPVLIDKFLDNSIELDVDAVSDGKDVLIGAVMEHIEEAGVHSGDSATSIP
HHHHHCCCCCCCEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYSLSKEIVEEVKEQTRKLAVALEVKGLINVQYAVQNNEVYVLEVNPRASRTVPFVSKSI
CCHHHHHHHHHHHHHHHHEEEEEEECCEEEEEEEEECCEEEEEEECCCCCCCCCHHHHHH
GYPLAKIATKVAIGKSLREILPEVFERLEKGEAHFASDFLPKEKKIFSVKEVVFPWKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCC
EVDPILGPEMKSTGEVMGIDKEFGLAYYKAQLSAGYRLPEKGNLFISVADRDKPKILELA
CCCCCCCCCCCCCCCEEECCHHHCHHHEEHHHHCCCCCCCCCCEEEEECCCCCHHHHHHH
KEFEKLGFGIYATSGTYKFLKEHGVNAKRVLKVSEGRPNVVDMIINGEIHLVINTPSGKR
HHHHHCCCEEEECCCHHHHHHHCCCCHHHEEEECCCCCCEEEEEECCEEEEEEECCCCCC
EKSDAYYIRRACVQFNVPYYTTMRAGYAVLEAIKSIKKLKEEGKGLSVHSLQEIYNI
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320