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The map label for this gene is gcsH4

Identifier: 15606376

GI number: 15606376

Start: 774106

End: 774621

Strand: Direct

Name: gcsH4

Synonym: aq_1108

Alternate gene names: 15606376

Gene position: 774106-774621 (Clockwise)

Preceding gene: 15607139

Following gene: 15606377

Centisome position: 49.9

GC content: 46.32

Gene sequence:

>516_bases
ATGGAAGACTTCTACGTTGAAGATTACCTTGTAAAGGGAGATCGCTACTACACAAAAGAGCACGAGTGGGTCAGGGTTAA
AAACGGCTTTGCGGAAGTTGGTATAACAGATTACGCCCAGAAACAACTGGGAGACATAGTTTACGTGGACCTTCCCGAGA
AGGGAAAGGAGGTGGATGCGGGAGACACCTTGGCTAACATAGAGTCCGTTAAAAACGTAGCTCCCGTTTACGCACCCGTT
ACGGGCACAGTTGTGGAGGTAAACGAGGACCTCAAAGACGAGCCGGGGATAATAAACGACGACCCGTATGAGGCGGGCTG
GATAGCTGTTATAGAGATGAAGGATCCCACGGAAGTGGAAGATTTGATGACAGCACAGGATTACGCAGAGTACCTCAAAG
AAATTGTAGAAGAGGAAAAGGAAGAAGAAGTTGAAGTAAAAGAAGAGGAATTAATAGAAACCGAGTCCATAGAAGAGTTG
TCCGAAGAAGAACTCGGATACGAGGAGAATAAATGA

Upstream 100 bases:

>100_bases
ACGAAGAGTACGTGGATGAGGACTATAAAATCTCCGACGGGGATAGGGTTGCTTTAATACCTCCAGTTAGCGGTGGTTAA
AACTTCTTGGAGGTGGAAGG

Downstream 100 bases:

>100_bases
GTTACATTCCTCATTCGGAGGAAGAAACAAAAGAGATACTCTCAAAACTCGGTCTAGAAAGCTTAGAGGATCTCTTTTCC
CACATACCAAAAGAGCTTTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MEDFYVEDYLVKGDRYYTKEHEWVRVKNGFAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPV
TGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMKDPTEVEDLMTAQDYAEYLKEIVEEEKEEEVEVKEEELIETESIEEL
SEEELGYEENK

Sequences:

>Translated_171_residues
MEDFYVEDYLVKGDRYYTKEHEWVRVKNGFAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPV
TGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMKDPTEVEDLMTAQDYAEYLKEIVEEEKEEEVEVKEEELIETESIEEL
SEEELGYEENK
>Mature_171_residues
MEDFYVEDYLVKGDRYYTKEHEWVRVKNGFAEVGITDYAQKQLGDIVYVDLPEKGKEVDAGDTLANIESVKNVAPVYAPV
TGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMKDPTEVEDLMTAQDYAEYLKEIVEEEKEEEVEVKEEELIETESIEEL
SEEELGYEENK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=41.3223140495868, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=40.495867768595, Blast_Score=117, Evalue=5e-27,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=47.2, Blast_Score=116, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=125, Percent_Identity=44.8, Blast_Score=130, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=43.2, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=42.5, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=43.6507936507937, Blast_Score=116, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH4_AQUAE (O67192)

Other databases:

- EMBL:   AE000657
- PIR:   E70395
- RefSeq:   NP_213756.1
- ProteinModelPortal:   O67192
- SMR:   O67192
- GeneID:   1193826
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_1108
- NMPDR:   fig|224324.1.peg.771
- HOGENOM:   HBG693789
- OMA:   QGAGWFF
- PhylomeDB:   O67192
- ProtClustDB:   CLSK2299599
- BioCyc:   AAEO224324:AQ_1108-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 19613; Mature: 19613

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDFYVEDYLVKGDRYYTKEHEWVRVKNGFAEVGITDYAQKQLGDIVYVDLPEKGKEVDA
CCCCCCCCEEECCCEEECCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCC
GDTLANIESVKNVAPVYAPVTGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMKDPTEVE
CCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
DLMTAQDYAEYLKEIVEEEKEEEVEVKEEELIETESIEELSEEELGYEENK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEDFYVEDYLVKGDRYYTKEHEWVRVKNGFAEVGITDYAQKQLGDIVYVDLPEKGKEVDA
CCCCCCCCEEECCCEEECCCCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCC
GDTLANIESVKNVAPVYAPVTGTVVEVNEDLKDEPGIINDDPYEAGWIAVIEMKDPTEVE
CCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHH
DLMTAQDYAEYLKEIVEEEKEEEVEVKEEELIETESIEELSEEELGYEENK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320