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The map label for this gene is rfbD [H]

Identifier: 15606359

GI number: 15606359

Start: 754007

End: 755044

Strand: Direct

Name: rfbD [H]

Synonym: aq_1082

Alternate gene names: 15606359

Gene position: 754007-755044 (Clockwise)

Preceding gene: 15606357

Following gene: 15606364

Centisome position: 48.6

GC content: 45.28

Gene sequence:

>1038_bases
ATGTCTGGAAAGCGTGCTTTAATTACAGGAATCAGGGGGCAGGATGGAGCTTACTTGGCTAAGCTCTTATTAGAAAAGGG
ATACGAGGTTTACGGAGCTGATAGGAGAAGCGGAGAATTTGCGAGCTGGAGACTGAAAGAACTCGGGATAGAAAACGACG
TAAAGATCATTCACATGGATCTGCTTGAGTTTTCAAACATTATCAGAACCATAGAAAAGGTTCAACCCGATGAAGTTTAC
AACTTAGCAGCTCAGAGCTTTGTGGGAGTTTCCTTTGAACAGCCCATACTTACCGCGGAAGTAGATGCCATAGGAGTTCT
GAGAATTCTTGAAGCTCTGAGAACAGTAAAACCTGACACCAAGTTTTATCAGGCTTCCACGAGCGAGATGTTCGGTAAGG
TGCAGGAAATTCCCCAAACGGAAAAAACGCCTTTTTACCCCAGAAGTCCTTATGCGGTTGCCAAACTCTTCGGACACTGG
ATAACGGTAAATTACAGGGAAGCTTATAACATGTTCGCCTGCAGTGGGATACTCTTTAACCACGAATCACCCCTAAGGGG
TATTGAATTTGTGACGAGAAAGATAACATACAGCTTGGCGAGGATAAAGTACGGACTTCAGGATAAGTTAGTTCTCGGAA
ACTTAAATGCCAAGAGGGACTGGGGATACGCACCCGAATACGTGGAAGCTATGTGGCTTATGATGCAGCAGCCGGAACCA
GATGACTACGTTATAGCGACTGGGGAAACCCATACCGTTCGGGAATTCGTAGAAAAAGCGGCGAAAATAGCGGGGTTCGA
CATAGAGTGGGTCGGTGAAGGGATAAACGAGAAAGGAATAGACAGAAACACAGGTAAGGTGATAGTGGAAGTCTCGGAAG
AATTCTTCAGACCCGCGGAAGTGGACATACTTGTAGGAAATCCCGAAAAGGCTATGAAGAAGCTCGGATGGAAACCGAGA
ACCACTTTTGACGAACTCGTTGAAATAATGATGGAAGCGGACCTTAAAAGAGTAAGAGACAGAGAAGTTAGCGTTTAA

Upstream 100 bases:

>100_bases
ATTTTTTCCAAAACTTCTTTTTTCTCCTCTTCATCGGATATAATTAAGCTAACTAGATCTTCCTTTACCTCTATAAGAAT
TGAGTATACAATAGAAAATC

Downstream 100 bases:

>100_bases
CCTTAAAGCTCTTCAAAGGATTCAGCTATATTTCTGAGCTGGTGCATCGTTGCCCTTTTTACCAATTTCTTTGCCTGAGT
TTCGGGTTCCTTTTCAAGGA

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSGKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMDLLEFSNIIRTIEKVQPDEVY
NLAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVKPDTKFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHW
ITVNYREAYNMFACSGILFNHESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEP
DDYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAEVDILVGNPEKAMKKLGWKPR
TTFDELVEIMMEADLKRVRDREVSV

Sequences:

>Translated_345_residues
MSGKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMDLLEFSNIIRTIEKVQPDEVY
NLAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVKPDTKFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHW
ITVNYREAYNMFACSGILFNHESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEP
DDYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAEVDILVGNPEKAMKKLGWKPR
TTFDELVEIMMEADLKRVRDREVSV
>Mature_344_residues
SGKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMDLLEFSNIIRTIEKVQPDEVYN
LAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVKPDTKFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHWI
TVNYREAYNMFACSGILFNHESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEPD
DYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAEVDILVGNPEKAMKKLGWKPRT
TFDELVEIMMEADLKRVRDREVSV

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=56.140350877193, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=52.6610644257703, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1788353, Length=304, Percent_Identity=24.3421052631579, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=24.198250728863, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=55.1319648093842, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=55.1319648093842, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=55.1319648093842, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=55.1319648093842, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=55.1319648093842, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=54.8387096774194, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=54.1547277936963, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39339; Mature: 39207

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMD
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEHH
LLEFSNIIRTIEKVQPDEVYNLAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVKPDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
KFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHWITVNYREAYNMFACSGILFN
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEEEHHHHHHHHHHCEEEEC
HESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
DDYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAE
CCEEEECCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHCCCCE
VDILVGNPEKAMKKLGWKPRTTFDELVEIMMEADLKRVRDREVSV
EEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGKRALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGEFASWRLKELGIENDVKIIHMD
CCCEEEEEECCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCCCEEEEHH
LLEFSNIIRTIEKVQPDEVYNLAAQSFVGVSFEQPILTAEVDAIGVLRILEALRTVKPDT
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCC
KFYQASTSEMFGKVQEIPQTEKTPFYPRSPYAVAKLFGHWITVNYREAYNMFACSGILFN
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEEEEHHHHHHHHHHCEEEEC
HESPLRGIEFVTRKITYSLARIKYGLQDKLVLGNLNAKRDWGYAPEYVEAMWLMMQQPEP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
DDYVIATGETHTVREFVEKAAKIAGFDIEWVGEGINEKGIDRNTGKVIVEVSEEFFRPAE
CCEEEECCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHCCCCE
VDILVGNPEKAMKKLGWKPRTTFDELVEIMMEADLKRVRDREVSV
EEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]