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The map label for this gene is proA [H]

Identifier: 15606350

GI number: 15606350

Start: 742979

End: 744310

Strand: Direct

Name: proA [H]

Synonym: aq_1071

Alternate gene names: 15606350

Gene position: 742979-744310 (Clockwise)

Preceding gene: 15606349

Following gene: 15606351

Centisome position: 47.89

GC content: 45.57

Gene sequence:

>1332_bases
ATGACTGCGGTAAGCCAAGAAATAATGAACTACATAGAGGAGAAAGTAAACAGGGCAAGAAGCGTTCTGAGAGAACTCAC
TTCCCTCAAAACTTCCGTAAAGAACGAAACCCTCTTAAAAGTAGCAGAACTAATAGATAAAAACAGGGATTACATAAAGG
AAGAAAACAGGAAGGACGTAGAAAAGGCAAAGGAAATGGGATTGAGACCTGCAGTTGTAGACAGGTTGGTTCTCAACGAC
AAGAGAATAGACGGAATGGTAAAAGTACTCAAAGACGTTGCCTCCTTACCTGATCCTGTAGGTGAGATTATAAAAATGTG
GAATCTCCCTAACGGTCTGAAAGTGGGAAGGATGAGAGTTCCCTTGGGAGTGATATTCATCGTTTACGAGTCAAGGCCGA
ACGTCACTATAGAAGCCTCATCCCTTTGTATGAAGTCCTCAAACGCAGTTATCCTGAGAGGAGGCAAAGAAGCCATAAAC
TCTAACAAAGCACTCGTTAACCTAATAAAGGAAGCGTGCAGGGAAACGGGATTTCCCGAAGATGCTGTTCAGTTCATAGA
CAGATCCGAGAGGGAAATAGTGTGGGAAATCCTGAAGATGGAGGGCAAAATAGACGTTGCCATTCCGAGGGGAGGGGAAA
GCTTGATAAGGGCTGTTGTTGAACACGCAAAAGTTCCCGTCATTAAACACTACAAGGGTGTTTGCAACATTTACGTTGAC
GACGAGGCTGACCTTGAAAAGGCTTACCACATAGTTTACAACGCAAAGGTTCAAAGGCCTTCCGTCTGCAACGCTGTTGA
GAACCTAGTAATAAACAGAAAAATACTGAAAGAGTTCTTCCCGAAGATGGCTTACTACCTCGGAAAGGCGGGTGTGGAAC
TCAGGTGTGATGAAGAAAGCCTGCAGGTTATAAAGGAAAACCCGAAACTCTCCTTTGTTAACGCAAAACCTGCAACTGAA
GAGGATTACTACGAGGAGTTCTTAGACTTAATACTCGCCGTGAAGGTGGTAGACGATGTTGACGAAGCGATAGCTTTCAT
AGAAAAGTACGGCTCAAAACACTCGGATGCGATAATCACGGAAAACTACACAAAGGCTATGAAGTTCTTAAGGGAAGTGG
ACTCCGCCGCCGTTTACGTTAACGCGTCCACGAGATTTACCGACGGAAACGAGTTCGGACTCGGAGCGGAAATGGGGATC
TCAACGGACAAGATCCATGCAAGGGGGCCCATGGCACTCGAAGAGCTGACCATACCGAAGTTCGTAATACTCGGAGAAGG
GCAGGTGAGGGACAACTTCGGTGTTCCCGAAGAGTGGATGAAGGAGTTTTAA

Upstream 100 bases:

>100_bases
AATAAATTTTTACGCTTATCTCTCAAGGAAGTTTAAAAAGCAAGTACCGAAGTTAAAGTTCGTGCTTTAGTGTGAGTAAC
ATCTGTATATATTAAAACTT

Downstream 100 bases:

>100_bases
AGGAGGCTTGTGATGACTCAAAAGAAGCCCATAAGGGATCTCATTGAAAGCTGTATAACAAAAGTAGAGGAACACTACAA
GTTAAAGGAACTCTACCAGA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTAVSQEIMNYIEEKVNRARSVLRELTSLKTSVKNETLLKVAELIDKNRDYIKEENRKDVEKAKEMGLRPAVVDRLVLND
KRIDGMVKVLKDVASLPDPVGEIIKMWNLPNGLKVGRMRVPLGVIFIVYESRPNVTIEASSLCMKSSNAVILRGGKEAIN
SNKALVNLIKEACRETGFPEDAVQFIDRSEREIVWEILKMEGKIDVAIPRGGESLIRAVVEHAKVPVIKHYKGVCNIYVD
DEADLEKAYHIVYNAKVQRPSVCNAVENLVINRKILKEFFPKMAYYLGKAGVELRCDEESLQVIKENPKLSFVNAKPATE
EDYYEEFLDLILAVKVVDDVDEAIAFIEKYGSKHSDAIITENYTKAMKFLREVDSAAVYVNASTRFTDGNEFGLGAEMGI
STDKIHARGPMALEELTIPKFVILGEGQVRDNFGVPEEWMKEF

Sequences:

>Translated_443_residues
MTAVSQEIMNYIEEKVNRARSVLRELTSLKTSVKNETLLKVAELIDKNRDYIKEENRKDVEKAKEMGLRPAVVDRLVLND
KRIDGMVKVLKDVASLPDPVGEIIKMWNLPNGLKVGRMRVPLGVIFIVYESRPNVTIEASSLCMKSSNAVILRGGKEAIN
SNKALVNLIKEACRETGFPEDAVQFIDRSEREIVWEILKMEGKIDVAIPRGGESLIRAVVEHAKVPVIKHYKGVCNIYVD
DEADLEKAYHIVYNAKVQRPSVCNAVENLVINRKILKEFFPKMAYYLGKAGVELRCDEESLQVIKENPKLSFVNAKPATE
EDYYEEFLDLILAVKVVDDVDEAIAFIEKYGSKHSDAIITENYTKAMKFLREVDSAAVYVNASTRFTDGNEFGLGAEMGI
STDKIHARGPMALEELTIPKFVILGEGQVRDNFGVPEEWMKEF
>Mature_442_residues
TAVSQEIMNYIEEKVNRARSVLRELTSLKTSVKNETLLKVAELIDKNRDYIKEENRKDVEKAKEMGLRPAVVDRLVLNDK
RIDGMVKVLKDVASLPDPVGEIIKMWNLPNGLKVGRMRVPLGVIFIVYESRPNVTIEASSLCMKSSNAVILRGGKEAINS
NKALVNLIKEACRETGFPEDAVQFIDRSEREIVWEILKMEGKIDVAIPRGGESLIRAVVEHAKVPVIKHYKGVCNIYVDD
EADLEKAYHIVYNAKVQRPSVCNAVENLVINRKILKEFFPKMAYYLGKAGVELRCDEESLQVIKENPKLSFVNAKPATEE
DYYEEFLDLILAVKVVDDVDEAIAFIEKYGSKHSDAIITENYTKAMKFLREVDSAAVYVNASTRFTDGNEFGLGAEMGIS
TDKIHARGPMALEELTIPKFVILGEGQVRDNFGVPEEWMKEF

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=426, Percent_Identity=37.5586854460094, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI21361368, Length=426, Percent_Identity=37.5586854460094, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1786438, Length=413, Percent_Identity=45.2784503631961, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569969, Length=427, Percent_Identity=38.6416861826698, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17569967, Length=427, Percent_Identity=38.6416861826698, Blast_Score=285, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6324899, Length=440, Percent_Identity=36.3636363636364, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI21357643, Length=436, Percent_Identity=36.4678899082569, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 49979; Mature: 49848

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVSQEIMNYIEEKVNRARSVLRELTSLKTSVKNETLLKVAELIDKNRDYIKEENRKDV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
EKAKEMGLRPAVVDRLVLNDKRIDGMVKVLKDVASLPDPVGEIIKMWNLPNGLKVGRMRV
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEC
PLGVIFIVYESRPNVTIEASSLCMKSSNAVILRGGKEAINSNKALVNLIKEACRETGFPE
CEEEEEEEEECCCCEEEEHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHCCCCH
DAVQFIDRSEREIVWEILKMEGKIDVAIPRGGESLIRAVVEHAKVPVIKHYKGVCNIYVD
HHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEC
DEADLEKAYHIVYNAKVQRPSVCNAVENLVINRKILKEFFPKMAYYLGKAGVELRCDEES
CCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHH
LQVIKENPKLSFVNAKPATEEDYYEEFLDLILAVKVVDDVDEAIAFIEKYGSKHSDAIIT
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ENYTKAMKFLREVDSAAVYVNASTRFTDGNEFGLGAEMGISTDKIHARGPMALEELTIPK
CCHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCE
FVILGEGQVRDNFGVPEEWMKEF
EEEECCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
TAVSQEIMNYIEEKVNRARSVLRELTSLKTSVKNETLLKVAELIDKNRDYIKEENRKDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
EKAKEMGLRPAVVDRLVLNDKRIDGMVKVLKDVASLPDPVGEIIKMWNLPNGLKVGRMRV
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEC
PLGVIFIVYESRPNVTIEASSLCMKSSNAVILRGGKEAINSNKALVNLIKEACRETGFPE
CEEEEEEEEECCCCEEEEHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHCCCCH
DAVQFIDRSEREIVWEILKMEGKIDVAIPRGGESLIRAVVEHAKVPVIKHYKGVCNIYVD
HHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCHHHHCCCEEEEEEC
DEADLEKAYHIVYNAKVQRPSVCNAVENLVINRKILKEFFPKMAYYLGKAGVELRCDEES
CCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHH
LQVIKENPKLSFVNAKPATEEDYYEEFLDLILAVKVVDDVDEAIAFIEKYGSKHSDAIIT
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ENYTKAMKFLREVDSAAVYVNASTRFTDGNEFGLGAEMGISTDKIHARGPMALEELTIPK
CCHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCE
FVILGEGQVRDNFGVPEEWMKEF
EEEECCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]