BacMap

Definition	Aquifex aeolicus VF5, complete genome.
Accession	NC_000918
Length	1,551,335

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The map label for this gene is selB [H]

Identifier: 15606325

GI number: 15606325

Start: 716314

End: 718062

Strand: Direct

Name: selB [H]

Synonym: aq_1033

Alternate gene names: 15606325

Gene position: 716314-718062 (Clockwise)

Preceding gene: 15606318

Following gene: 15606329

Centisome position: 46.17

GC content: 33.1

Gene sequence:

>1749_bases
ATGAAGTATATTTTATTTGCAACGGCAGGTCATGTTGATCATGGAAAAACGACTTTGATAAAAACCCTCACAGGCATTGA
TACAGATAGGCTTCCTGAAGAAAAGAAGAGAGGGCTTAGTATCGATATAGGGTTTGCTTATATAGATTTCCCCGATATAA
ATACAAGGTTAGAGATCATTGACGTTCCTGGACATGAAAGGTTTATAAAAAATGCTATAGCGGGTATTTGTTCAGCATCT
GGATTGATTCTCGTAGTAGATCCAAATGAAGGGATAATGCCACAAACAATAGAACACCTAAGAGTAGCAAAAAGTTTTGG
TATAAAACATGGTATAGCTGTATTGACTAAGATGGATAAAGTAGATGAAGAACTTGCACATATAGCAGAAGAAGAGTTAA
TAGCATTTCTGGAAAAGGAAGAAATGAATATGGAAATCGTCAAAGTCTCTGCAGTAACAGGGCAAGGCATTGAAGATTTA
AAAAATTCTATAAAAAAGCTATTGGAAAGTATTAATAATCTGAATAAACATAAGCCGCTACGTATATTTGTAGATTCTGC
GTTTGTTGTAAAAGGATACGGGACAGTTTTAAGAGGAAGCTGTTTTGAAGGAGAGGTTAAGGAAGGTGATAAGGTAGTAG
TAGAACCTATAGGGGTTATTTCAAGAGTTAGAAAAATGCAGAACCACGGAGTGTTCGTAAAGAAAGCTGTTGCAGGAGAA
AGAATTGCTTTAAATTTACCCGAAGTGGATGCAAAAAAAGTAAAAAGAGGCTTTTTAATTTTAAAGCCTGAAAGTTATGA
GAAGAGTAATGTTCTTATTGTAAAAACCGAAATAGATCTTAAACCAGGGAAAATATATCAAGTATTTTTCGGTATGAGAG
AAACGGTTGGAAAAATAAGTGTTATTGATAAGGGTATTTATCTGGTCAGACTTAAGGAAAATGCAATTGTACGCAGGGGA
GACAAATTAGTCGTACTTGATTCTTCCGGAAACTTTTTAGGAGGTGCAGAAGTATTACATCCAAAAGTGAGAGTAACTAA
AAAAGCTTTTATAAAAAAGAATATAAAAGATTTATTAGAAAACTTTGAATGTTATTTGCTGAAGGAAAGGGGACCCATAG
GTCTAAAACTTGAGTTCTTTAAGAGAATTACAGGAGTAAGCCCAAAAGTAGCAAATTTGAAACCTGAAAGTATTGAAATA
AGAGGGGTTTATTACTTGAAAGGATTTATTGAAAACTTAAAATTAAAGATAAAAAAATTTTTGGATACAGAACTTCAAAA
CGCTTTTGGTGTTGATAAAGAAAAAGTTAAAAGTATGTTTTCCTTAAATGAAGAATTACTCAAGTATATACTTGATGAAC
TAAAAACCTATAAAATTGTCAACGAATTAATAATTGACGAAAGAAAAAGTGATCTTGAGAAGAATGAAGACTTTCAAAAG
TTAATGTCTATTCTTAAAGGAGGAATAAAAGAGGAAAGGGAAATTATATTGGAAGGAATACCAAAGGAAATTCTCACTCT
TTCTATAAAAAGAAAATACGCTCATAGAATAGGTGAATATCTCATAATATCCGATGAACTCTTAAAAAAGTATATTAATG
AGCTGAAAGAACTTGGAAAGACGTTCAATGTTCAACAAGCTAAAAATAAACTTGGATTAACGAGGAAGTACCTTATCCCG
CTTCTTGAGTATCTCGACTACTTAGGACTTACGGTAAGGGAAGGGAACGAGAGGAGGTGGAAGCGCTAA

Upstream 100 bases:

>100_bases
TTTCTGGATATTTTTTTTTAAATATTTCAACTACTTTTGATATTTGTGGTATCTGTCTGAGTAGACTTTTCATAGATATT
AAATTTAATTAATAAGAGCA

Downstream 100 bases:

>100_bases
GCCCTCACCAGTTTTATCCTGTAAACGAAGCTCTTTGGCTCGAGCCAGTTCAGGGGTTCACCTTTCACCTCCGCGTAAGG
GTATCCAAACAGGTTCAGGG

Product: elongation factor SelB

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDFPDINTRLEIIDVPGHERFIKNAIAGICSAS
GLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDKVDEELAHIAEEELIAFLEKEEMNMEIVKVSAVTGQGIEDL
KNSIKKLLESINNLNKHKPLRIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGE
RIALNLPEVDAKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKISVIDKGIYLVRLKENAIVRRG
DKLVVLDSSGNFLGGAEVLHPKVRVTKKAFIKKNIKDLLENFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEI
RGVYYLKGFIENLKLKIKKFLDTELQNAFGVDKEKVKSMFSLNEELLKYILDELKTYKIVNELIIDERKSDLEKNEDFQK
LMSILKGGIKEEREIILEGIPKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGKTFNVQQAKNKLGLTRKYLIP
LLEYLDYLGLTVREGNERRWKR

Sequences:

>Translated_582_residues
MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDFPDINTRLEIIDVPGHERFIKNAIAGICSAS
GLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDKVDEELAHIAEEELIAFLEKEEMNMEIVKVSAVTGQGIEDL
KNSIKKLLESINNLNKHKPLRIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGE
RIALNLPEVDAKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKISVIDKGIYLVRLKENAIVRRG
DKLVVLDSSGNFLGGAEVLHPKVRVTKKAFIKKNIKDLLENFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEI
RGVYYLKGFIENLKLKIKKFLDTELQNAFGVDKEKVKSMFSLNEELLKYILDELKTYKIVNELIIDERKSDLEKNEDFQK
LMSILKGGIKEEREIILEGIPKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGKTFNVQQAKNKLGLTRKYLIP
LLEYLDYLGLTVREGNERRWKR
>Mature_582_residues
MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDFPDINTRLEIIDVPGHERFIKNAIAGICSAS
GLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDKVDEELAHIAEEELIAFLEKEEMNMEIVKVSAVTGQGIEDL
KNSIKKLLESINNLNKHKPLRIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGE
RIALNLPEVDAKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKISVIDKGIYLVRLKENAIVRRG
DKLVVLDSSGNFLGGAEVLHPKVRVTKKAFIKKNIKDLLENFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEI
RGVYYLKGFIENLKLKIKKFLDTELQNAFGVDKEKVKSMFSLNEELLKYILDELKTYKIVNELIIDERKSDLEKNEDFQK
LMSILKGGIKEEREIILEGIPKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGKTFNVQQAKNKLGLTRKYLIP
LLEYLDYLGLTVREGNERRWKR

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=294, Percent_Identity=30.952380952381, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI54607086, Length=311, Percent_Identity=25.7234726688103, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI4503471, Length=312, Percent_Identity=26.6025641025641, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223555963, Length=328, Percent_Identity=24.390243902439, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI5729864, Length=375, Percent_Identity=23.7333333333333, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI4503475, Length=312, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194018522, Length=311, Percent_Identity=25.08038585209, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI194097354, Length=309, Percent_Identity=25.2427184466019, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI194018520, Length=309, Percent_Identity=25.2427184466019, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI157426893, Length=226, Percent_Identity=29.646017699115, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI46094014, Length=318, Percent_Identity=25.4716981132075, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729339, Length=209, Percent_Identity=27.7511961722488, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI53729337, Length=209, Percent_Identity=27.7511961722488, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367247, Length=618, Percent_Identity=27.6699029126214, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1790412, Length=292, Percent_Identity=29.4520547945205, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789737, Length=292, Percent_Identity=29.4520547945205, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789108, Length=350, Percent_Identity=27.1428571428571, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788922, Length=343, Percent_Identity=25.0728862973761, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=290, Percent_Identity=30.6896551724138, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556456, Length=314, Percent_Identity=29.2993630573248, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17552884, Length=312, Percent_Identity=27.8846153846154, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17569207, Length=312, Percent_Identity=27.8846153846154, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566629, Length=318, Percent_Identity=27.6729559748428, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506081, Length=286, Percent_Identity=27.6223776223776, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115532067, Length=333, Percent_Identity=27.3273273273273, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115532065, Length=333, Percent_Identity=27.3273273273273, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=292, Percent_Identity=30.8219178082192, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325337, Length=310, Percent_Identity=28.0645161290323, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319594, Length=310, Percent_Identity=28.0645161290323, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=387, Percent_Identity=24.031007751938, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=234, Percent_Identity=29.9145299145299, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324550, Length=211, Percent_Identity=27.0142180094787, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=290, Percent_Identity=32.4137931034483, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17864358, Length=290, Percent_Identity=32.4137931034483, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921738, Length=305, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922690, Length=315, Percent_Identity=30.4761904761905, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24652838, Length=314, Percent_Identity=26.7515923566879, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137572, Length=314, Percent_Identity=26.7515923566879, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45553807, Length=314, Percent_Identity=25.796178343949, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45553816, Length=314, Percent_Identity=25.796178343949, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651721, Length=314, Percent_Identity=25.796178343949, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864154, Length=314, Percent_Identity=25.796178343949, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550900, Length=322, Percent_Identity=26.7080745341615, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137380, Length=313, Percent_Identity=23.3226837060703, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28572034, Length=211, Percent_Identity=27.4881516587678, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 66176; Mature: 66176

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDFPDINTRLEII
CCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEEEEEEEEECCCCCCEEEEE
DVPGHERFIKNAIAGICSASGLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDK
ECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
VDEELAHIAEEELIAFLEKEEMNMEIVKVSAVTGQGIEDLKNSIKKLLESINNLNKHKPL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCE
RIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGE
EEEEECEEEEECCCHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCC
RIALNLPEVDAKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKIS
EEEEECCCCCHHHHHCCEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEE
VIDKGIYLVRLKENAIVRRGDKLVVLDSSGNFLGGAEVLHPKVRVTKKAFIKKNIKDLLE
EEECCEEEEEEECCHHEECCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHC
NFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEIRGVYYLKGFIENLKLKIKKF
CCEEEEEECCCCCCEEHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LDTELQNAFGVDKEKVKSMFSLNEELLKYILDELKTYKIVNELIIDERKSDLEKNEDFQK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LMSILKGGIKEEREIILEGIPKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGK
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHH
TFNVQQAKNKLGLTRKYLIPLLEYLDYLGLTVREGNERRWKR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCCCCC
>Mature Secondary Structure
MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDFPDINTRLEII
CCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEEEEEEEEECCCCCCEEEEE
DVPGHERFIKNAIAGICSASGLILVVDPNEGIMPQTIEHLRVAKSFGIKHGIAVLTKMDK
ECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
VDEELAHIAEEELIAFLEKEEMNMEIVKVSAVTGQGIEDLKNSIKKLLESINNLNKHKPL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCE
RIFVDSAFVVKGYGTVLRGSCFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGE
EEEEECEEEEECCCHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEECCCC
RIALNLPEVDAKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKIS
EEEEECCCCCHHHHHCCEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEE
VIDKGIYLVRLKENAIVRRGDKLVVLDSSGNFLGGAEVLHPKVRVTKKAFIKKNIKDLLE
EEECCEEEEEEECCHHEECCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHC
NFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEIRGVYYLKGFIENLKLKIKKF
CCEEEEEECCCCCCEEHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LDTELQNAFGVDKEKVKSMFSLNEELLKYILDELKTYKIVNELIIDERKSDLEKNEDFQK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LMSILKGGIKEEREIILEGIPKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGK
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHH
TFNVQQAKNKLGLTRKYLIPLLEYLDYLGLTVREGNERRWKR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8893853 [H]