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The map label for this gene is kefA [H]

Identifier: 15606312

GI number: 15606312

Start: 702655

End: 703965

Strand: Direct

Name: kefA [H]

Synonym: aq_1013

Alternate gene names: 15606312

Gene position: 702655-703965 (Clockwise)

Preceding gene: 15606311

Following gene: 15606314

Centisome position: 45.29

GC content: 43.4

Gene sequence:

>1311_bases
TTGAGTAAAACGTTTACGTTCCTCTACAAATTCTCTATTGCCCTGCTTTTGTGGATATTCTTAAGGACCCTTTTTAAAGT
CGCGATTAAAAGGTTTAAAGGTAGCGGTTTTGAATCCGTGCTTAATTCCCTCTGGTTTGTTTTGAGTGCGTCTTACGTGG
CGTTTACAGGCGCATGGATACTGAATCTCGTTAAGTTCTCCGTGGACTTTATAGTAGACGTTGCGGTATTTTTCGTAATT
TTCTTGGTCTACGCTGCGGTATTCGGTTACTCGGTTTATCTGATAGGTAAATTCTTCGGCGACACGGACGGTTACGAGAA
TCTCCTGAAGAGCTTTAAAAAGTTCGCTTATATGTTTTCCATATACATACTCTACAAAAGCCTTATCTACTTGACGAACA
TTGAAGTCCTCGTAAAGAAGCTTGAAAGAATATACATACTTGAGACGGAACTCGTTAAGATTTCTCTCTTTTCCTTTGTG
GAAGGGATATACGTCTTCTTTGTTCTGAAGTACTTCTTTGAAACACTCGGAAACCTTTTCTACGTTGTGTATATGAAAAA
GGACCAAGAAGTAGAAGCGGGTTCCTTTAGAACAATTACCGTAAACCTCGGTTTACTCCTTTCTCTCCTGATAGCGGGTG
TGAGTATAGGGATTACGTGGAAAGCTGCTGTTCCCATAGCGGGCGCCCTCGGCATAGGTTTGGGTTTTGGACTTCAGACC
ATTTTCAACAACTACGTCAGCGGGTTTATACTCCTCCTTAGCAAAAACATAAAAGTGGGTGATTACATAGAAATTGAAGG
AAAGGCCGGCGCCGCTGTAGGAGCTGGAGGAGACACGATTTTCGGAAGAGTAGTCAGTATAAATATCCTTACAACCATTG
TAAGGACTTGGGACAACGTGGAAGTCGCCATACCAAACTCCGAATTCCTGTCGAGCAAAATAGTCAATTACTCTATGACT
AACCCAATAGTGAGGGTCAGGATTCCCTTCGGAGTTGCTTACTCTTCGGACCCAGAAAAGGTAAGGGAAGTGCTAATAAG
AGTGGCAAAGAGGATGCCCCACGTACTGCCATCTCCTGAACCGGACGTCTGGTTCCACGAAATGGGAGACAGTGCACTTA
TATTTATCCTGCTCGTATGGGTGGACCTGAGGAGGTTAAGGCGACTGAAAGCTTTAAAGAGTTCCATTTATTATGAGGCG
TGGAAGGAACTAAAAAAAGAGGGAATAGAAATACCGTTCCCGCAAAGGGACGTGTGGTTCAGGAACAAACTGAAGGTAGA
GATAGAGAAAGACATCAAGCCCGAGCAGTGA

Upstream 100 bases:

>100_bases
CCAAGCTTTTAATAAACATTTTTTCTATCCTCGGCGGTATTTCCTTTTTCATTATAAAACTGTCTCCCTTTACAAGAAGA
AAAATATAAAGTTTCTGCTA

Downstream 100 bases:

>100_bases
GCTCTTCTTCCTTTTCTACTTCTACGATAAGGTAAACCTTGTCCCCTTCCCTTACCTTATCTACCGTTTTCAAACCTATA
ACGCTTCCCTTTTGAGCCAC

Product: hypothetical protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSKTFTFLYKFSIALLLWIFLRTLFKVAIKRFKGSGFESVLNSLWFVLSASYVAFTGAWILNLVKFSVDFIVDVAVFFVI
FLVYAAVFGYSVYLIGKFFGDTDGYENLLKSFKKFAYMFSIYILYKSLIYLTNIEVLVKKLERIYILETELVKISLFSFV
EGIYVFFVLKYFFETLGNLFYVVYMKKDQEVEAGSFRTITVNLGLLLSLLIAGVSIGITWKAAVPIAGALGIGLGFGLQT
IFNNYVSGFILLLSKNIKVGDYIEIEGKAGAAVGAGGDTIFGRVVSINILTTIVRTWDNVEVAIPNSEFLSSKIVNYSMT
NPIVRVRIPFGVAYSSDPEKVREVLIRVAKRMPHVLPSPEPDVWFHEMGDSALIFILLVWVDLRRLRRLKALKSSIYYEA
WKELKKEGIEIPFPQRDVWFRNKLKVEIEKDIKPEQ

Sequences:

>Translated_436_residues
MSKTFTFLYKFSIALLLWIFLRTLFKVAIKRFKGSGFESVLNSLWFVLSASYVAFTGAWILNLVKFSVDFIVDVAVFFVI
FLVYAAVFGYSVYLIGKFFGDTDGYENLLKSFKKFAYMFSIYILYKSLIYLTNIEVLVKKLERIYILETELVKISLFSFV
EGIYVFFVLKYFFETLGNLFYVVYMKKDQEVEAGSFRTITVNLGLLLSLLIAGVSIGITWKAAVPIAGALGIGLGFGLQT
IFNNYVSGFILLLSKNIKVGDYIEIEGKAGAAVGAGGDTIFGRVVSINILTTIVRTWDNVEVAIPNSEFLSSKIVNYSMT
NPIVRVRIPFGVAYSSDPEKVREVLIRVAKRMPHVLPSPEPDVWFHEMGDSALIFILLVWVDLRRLRRLKALKSSIYYEA
WKELKKEGIEIPFPQRDVWFRNKLKVEIEKDIKPEQ
>Mature_435_residues
SKTFTFLYKFSIALLLWIFLRTLFKVAIKRFKGSGFESVLNSLWFVLSASYVAFTGAWILNLVKFSVDFIVDVAVFFVIF
LVYAAVFGYSVYLIGKFFGDTDGYENLLKSFKKFAYMFSIYILYKSLIYLTNIEVLVKKLERIYILETELVKISLFSFVE
GIYVFFVLKYFFETLGNLFYVVYMKKDQEVEAGSFRTITVNLGLLLSLLIAGVSIGITWKAAVPIAGALGIGLGFGLQTI
FNNYVSGFILLLSKNIKVGDYIEIEGKAGAAVGAGGDTIFGRVVSINILTTIVRTWDNVEVAIPNSEFLSSKIVNYSMTN
PIVRVRIPFGVAYSSDPEKVREVLIRVAKRMPHVLPSPEPDVWFHEMGDSALIFILLVWVDLRRLRRLKALKSSIYYEAW
KELKKEGIEIPFPQRDVWFRNKLKVEIEKDIKPEQ

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=225, Percent_Identity=36.4444444444444, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI2367355, Length=266, Percent_Identity=28.9473684210526, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789291, Length=269, Percent_Identity=25.2788104089219, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787591, Length=268, Percent_Identity=23.134328358209, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49735; Mature: 49604

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTFTFLYKFSIALLLWIFLRTLFKVAIKRFKGSGFESVLNSLWFVLSASYVAFTGAWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNLVKFSVDFIVDVAVFFVIFLVYAAVFGYSVYLIGKFFGDTDGYENLLKSFKKFAYMFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IYILYKSLIYLTNIEVLVKKLERIYILETELVKISLFSFVEGIYVFFVLKYFFETLGNLF
HHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
YVVYMKKDQEVEAGSFRTITVNLGLLLSLLIAGVSIGITWKAAVPIAGALGIGLGFGLQT
EEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
IFNNYVSGFILLLSKNIKVGDYIEIEGKAGAAVGAGGDTIFGRVVSINILTTIVRTWDNV
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCE
EVAIPNSEFLSSKIVNYSMTNPIVRVRIPFGVAYSSDPEKVREVLIRVAKRMPHVLPSPE
EEEECCHHHHHHHHHCEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PDVWFHEMGDSALIFILLVWVDLRRLRRLKALKSSIYYEAWKELKKEGIEIPFPQRDVWF
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RNKLKVEIEKDIKPEQ
CCCEEEEEECCCCCCC
>Mature Secondary Structure 
SKTFTFLYKFSIALLLWIFLRTLFKVAIKRFKGSGFESVLNSLWFVLSASYVAFTGAWI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNLVKFSVDFIVDVAVFFVIFLVYAAVFGYSVYLIGKFFGDTDGYENLLKSFKKFAYMFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IYILYKSLIYLTNIEVLVKKLERIYILETELVKISLFSFVEGIYVFFVLKYFFETLGNLF
HHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
YVVYMKKDQEVEAGSFRTITVNLGLLLSLLIAGVSIGITWKAAVPIAGALGIGLGFGLQT
EEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
IFNNYVSGFILLLSKNIKVGDYIEIEGKAGAAVGAGGDTIFGRVVSINILTTIVRTWDNV
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCE
EVAIPNSEFLSSKIVNYSMTNPIVRVRIPFGVAYSSDPEKVREVLIRVAKRMPHVLPSPE
EEEECCHHHHHHHHHCEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PDVWFHEMGDSALIFILLVWVDLRRLRRLKALKSSIYYEAWKELKKEGIEIPFPQRDVWF
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RNKLKVEIEKDIKPEQ
CCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]