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The map label for this gene is leuC

Identifier: 15606262

GI number: 15606262

Start: 647737

End: 649035

Strand: Direct

Name: leuC

Synonym: aq_940

Alternate gene names: 15606262

Gene position: 647737-649035 (Clockwise)

Preceding gene: 15606261

Following gene: 15606263

Centisome position: 41.75

GC content: 49.04

Gene sequence:

>1299_bases
ATGGGAATGACTATAACCGAGAAGATTCTGGCGGAACACGCTGGTAAGAAGGAAGTCCACCCCGGAGAACTCATAACTGC
AAAGATAGACCTCGCAATGGCAAACGATGTGACCGCACCCCTTGCCATAAAGATACTTGAGAAGTACGGAATAGATAAGG
TCTTTGACCCTGAGAGGATAGCCCTTGTTCTCTCACACTTCGTTCCCGCAAAGGACATAAAGTCCGCTGAGCAGGCAAAG
ATAGTAAGAGAATTCGTAAAAAAGCACGGTATAAAGTGGTTCTTTGAAGAGGGAGAAGGTATAGAACACGCCATACTCCC
CGAGCAAGGACTCGTCGTTCCCGGGGACCTCGTTGTAGGTGCGGACTCCCACACCTGCACTTACGGAGCTCTCGGAGCCT
TTGCAACGGGGGTCGGTTCCACCGATATAGCCTACGCTATGGCTACCGGAGAAGTTTGGCTCAGAGTTCCCGAAAGTATG
AAGTTCATCTTCTACGGAAAACTAAAACCATGGGTGATGGGAAAGGACCTAATCCTCTACACGATAGGGCAAATAGGGGT
TGACGGAGCACTCTACAGGGCTATGGAATTTGACGGAGAGACCATAAGAAACCTTTCTATGGAACAGAGGTTTACTATAG
CAAACATGGCGATAGAAGCGGGTGGAAAGAGCGGAATAATCTCTCCTGATGAAAAAACGATAGAATACGTAAAACAAAGG
GCAAAGAGACCATGGAAAGTTTACCAGAGCGACCCTGACGCTGAGTACCACTCCGTTTACGAGTGGGACGCTTCTCAGAT
AGAACCCTTAGTAGCGTGGCCCTACCTACCTTCAAACGTGCATCCCGTTTCTGAATCCACCCATATAACGATAGACCAGG
CATTTATAGGCTCCTGCACAAACGGAAGAATAGAGGACCTGAGAGTTGCAGCAAAGGTGTTTGAAGCTGCGCTAAAGCAA
GGAAAGAAAGTTCACCCTTACGTGAGGTGCATAGTAATTCCCGCGTCCAAGGCTATATACAAGCAAGCTTTGAAAGAAGG
ACTCATAGATATCTTTATGGAAGCCGGTTGCGTGGTTTCTACTTCTACGTGCGGTCCCTGCCTTGGTGGACACATGGGAG
TGCTTGCGGAAGGTGAAAGGTGTATATCCACATCTAACAGAAACTTCCCCGGAAGGATGGGTCATCCTAAGAGCGAAAGC
TACCTCGCAAACCCTGCCGTGGTCGCTGCGAGTGCAATAATGGGAAGAATTGCCCACCCCGACGAGGTGGTGAAAGCCGA
AACCCTTGCGGGTGTTTAA

Upstream 100 bases:

>100_bases
TTAAAGACAAGTGTAAGAAAGAGGAACTCTTTGACAAAGACAGGAAATCTGAGGAAAATAAAGAACTTAAATCTGAAGAA
GTTAAGGAGGAGGTAGTCTG

Downstream 100 bases:

>100_bases
TTCTTCTTTTCTTTATGCCCGTAAAGTTAAAGGTTTACTTAAAACCTGAGAAACCTATAAAATCAAGCCTTCTCACACCT
GAGGCGGTTCACGGTCTCTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERIALVLSHFVPAKDIKSAEQAK
IVREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVGADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESM
KFIFYGKLKPWVMGKDLILYTIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQR
AKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRVAAKVFEAALKQ
GKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVSTSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSES
YLANPAVVAASAIMGRIAHPDEVVKAETLAGV

Sequences:

>Translated_432_residues
MGMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERIALVLSHFVPAKDIKSAEQAK
IVREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVGADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESM
KFIFYGKLKPWVMGKDLILYTIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQR
AKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRVAAKVFEAALKQ
GKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVSTSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSES
YLANPAVVAASAIMGRIAHPDEVVKAETLAGV
>Mature_431_residues
GMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERIALVLSHFVPAKDIKSAEQAKI
VREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVGADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESMK
FIFYGKLKPWVMGKDLILYTIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQRA
KRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCTNGRIEDLRVAAKVFEAALKQG
KKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVSTSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSESY
LANPAVVAASAIMGRIAHPDEVVKAETLAGV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=449, Percent_Identity=28.5077951002227, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI8659555, Length=386, Percent_Identity=24.6113989637306, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=388, Percent_Identity=24.7422680412371, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1786259, Length=469, Percent_Identity=35.8208955223881, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI87081781, Length=350, Percent_Identity=24.5714285714286, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI2367097, Length=439, Percent_Identity=25.5125284738041, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=25.207756232687, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=446, Percent_Identity=28.0269058295964, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=409, Percent_Identity=28.6063569682152, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=26.7605633802817, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=397, Percent_Identity=24.9370277078086, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=460, Percent_Identity=34.5652173913044, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=35.2549889135255, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323335, Length=450, Percent_Identity=30.2222222222222, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=457, Percent_Identity=29.1028446389497, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28571643, Length=447, Percent_Identity=28.4116331096197, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=29.0243902439024, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.4150943396226, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=26.1455525606469, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_AQUAE (O67078)

Other databases:

- EMBL:   AE000657
- PIR:   C70381
- RefSeq:   NP_213641.1
- ProteinModelPortal:   O67078
- SMR:   O67078
- GeneID:   1193620
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_940
- NMPDR:   fig|224324.1.peg.656
- HOGENOM:   HBG330745
- OMA:   TDPRDFL
- PhylomeDB:   O67078
- ProtClustDB:   PRK00402
- BioCyc:   AAEO224324:AQ_940-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 47089; Mature: 46958

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERI
CCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHH
ALVLSHFVPAKDIKSAEQAKIVREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVG
HHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCEEECCCEEEE
ADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESMKFIFYGKLKPWVMGKDLILY
CCCCCCCHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCEECCCCEEEE
TIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQR
EHHHHCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
AKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCT
CCCCCEEECCCCCCCHHHEEECCHHHCCCEEECCCCCCCCCCCCCCCEEEEEHHHHCCCC
NGRIEDLRVAAKVFEAALKQGKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVS
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
TSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSESYLANPAVVAASAIMGRIAHP
CCCCCCHHCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
DEVVKAETLAGV
HHHHHHHHHCCC
>Mature Secondary Structure 
GMTITEKILAEHAGKKEVHPGELITAKIDLAMANDVTAPLAIKILEKYGIDKVFDPERI
CCCHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHH
ALVLSHFVPAKDIKSAEQAKIVREFVKKHGIKWFFEEGEGIEHAILPEQGLVVPGDLVVG
HHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCEEECCCEEEE
ADSHTCTYGALGAFATGVGSTDIAYAMATGEVWLRVPESMKFIFYGKLKPWVMGKDLILY
CCCCCCCHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCEECCCCEEEE
TIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYVKQR
EHHHHCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
AKRPWKVYQSDPDAEYHSVYEWDASQIEPLVAWPYLPSNVHPVSESTHITIDQAFIGSCT
CCCCCEEECCCCCCCHHHEEECCHHHCCCEEECCCCCCCCCCCCCCCEEEEEHHHHCCCC
NGRIEDLRVAAKVFEAALKQGKKVHPYVRCIVIPASKAIYKQALKEGLIDIFMEAGCVVS
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
TSTCGPCLGGHMGVLAEGERCISTSNRNFPGRMGHPKSESYLANPAVVAASAIMGRIAHP
CCCCCCHHCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
DEVVKAETLAGV
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320