Click here to switch to the map view.

The map label for this gene is ispE

Identifier: 15606244

GI number: 15606244

Start: 630432

End: 631238

Strand: Direct

Name: ispE

Synonym: aq_915

Alternate gene names: 15606244

Gene position: 630432-631238 (Clockwise)

Preceding gene: 15606243

Following gene: 15606245

Centisome position: 40.64

GC content: 45.23

Gene sequence:

>807_bases
ATGATAAAGGTTCTATCACCCGCGAAGATAAACTTGGGGCTGTGGGTTCTGGGGAGGCTACCGAGTGGTTACCACGAGAT
ACTTACCCTCTACCAGGAAATCCCCTTTTACGATGAGATTTACATAAGGGAGGGAGTGCTCAGGGTAGAGACGAATATAG
GCATTCCTCAGGAGGAGAACTTAGTTTACAAAGGATTGAGGGAATTTGAAAGGATAACAGGGATTGAAATTAATTACTCT
ATCTTTATTCAGAAGAATATACCACCCGGTGCGGGACTCGGAGGAGGAAGCTCAAATCTGGCGGTAGTTCTAAAAAAGGT
AAACGAACTCTTAGGATCTCCCTTATCTGAGGAAGAACTGAGGGAACTTGTAGGCAGTATTTCCGCAGACGCTCCCTTTT
TCCTTCTCGGAAAGAGCGCTATCGGAAGGGGAAAAGGGGAAGTTCTTGAACCCGTGGAAACTGAAATTTCGGGTAAAATC
ACACTAGTAATCCCTCAAGTCTCTTCAAGCACGGGAAGGGTTTACTCTTCCTTGAGGGAAGAACACTTCGTTACACCAGA
GTACGCGGAAGAAAAAATTCAAAGAATCATCTCGGGAGAGGTTGAGGAAATAGAAAACGTCCTCGGAGATATAGCGAGGG
AACTTTACCCGGAAATAAACGAGGTTTACAGGTTTGTTGAGTATTTGGGATTCAAGCCTTTTGTGAGCGGGAGCGGTTCA
ACGGTTTACTTCTTCGGAGGGGCATCGGAAGAGTTAAAAAAAGCTGCAAAGATGAGGGGCTGGAAAGTTGTAGAGCTAGA
ACTCTAA

Upstream 100 bases:

>100_bases
ATAGAGCTCGTGGAAAATCAGGTTTACCTGAGACTTTACGACAAAAATGGAAAAGTTGTTGAGGAGATAAAGTCGAGGGA
AGAGTTTTAGAATGTAAATT

Downstream 100 bases:

>100_bases
ACCTTTGATAAGATAACTCTAAAGGAGGAGATTATGGAAGAACAAAGATTTGTAGCAGTTCCTTTTGCGCTGGTGGGCTT
TTTCGTAACAGACAGGGACG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEENLVYKGLREFERITGIEINYS
IFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEELRELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKI
TLVIPQVSSSTGRVYSSLREEHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS
TVYFFGGASEELKKAAKMRGWKVVELEL

Sequences:

>Translated_268_residues
MIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEENLVYKGLREFERITGIEINYS
IFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEELRELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKI
TLVIPQVSSSTGRVYSSLREEHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS
TVYFFGGASEELKKAAKMRGWKVVELEL
>Mature_268_residues
MIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEENLVYKGLREFERITGIEINYS
IFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEELRELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKI
TLVIPQVSSSTGRVYSSLREEHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS
TVYFFGGASEELKKAAKMRGWKVVELEL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=253, Percent_Identity=34.3873517786561, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_AQUAE (O67060)

Other databases:

- EMBL:   AE000657
- PIR:   A70379
- RefSeq:   NP_213622.1
- PDB:   2V2Q
- PDB:   2V2V
- PDB:   2V2Z
- PDB:   2V34
- PDB:   2V8P
- PDB:   2VF3
- PDBsum:   2V2Q
- PDBsum:   2V2V
- PDBsum:   2V2Z
- PDBsum:   2V34
- PDBsum:   2V8P
- PDBsum:   2VF3
- ProteinModelPortal:   O67060
- SMR:   O67060
- GeneID:   1193602
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_915
- NMPDR:   fig|224324.1.peg.637
- HOGENOM:   HBG734593
- OMA:   YAKLNLY
- PhylomeDB:   O67060
- ProtClustDB:   PRK14611
- BioCyc:   AAEO224324:AQ_915-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29789; Mature: 29789

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEEN
CCEECCCCEECEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCH
LVYKGLREFERITGIEINYSIFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEEL
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
RELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKITLVIPQVSSSTGRVYSSLRE
HHHHHCCCCCCCEEEECCHHCCCCCCCCCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHH
EHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
TVYFFGGASEELKKAAKMRGWKVVELEL
EEEEECCCHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEEN
CCEECCCCEECEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCH
LVYKGLREFERITGIEINYSIFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEEL
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
RELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKITLVIPQVSSSTGRVYSSLRE
HHHHHCCCCCCCEEEECCHHCCCCCCCCCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHH
EHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCC
TVYFFGGASEELKKAAKMRGWKVVELEL
EEEEECCCHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320