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The map label for this gene is prfA

Identifier: 15606216

GI number: 15606216

Start: 604785

End: 605873

Strand: Direct

Name: prfA

Synonym: aq_876

Alternate gene names: 15606216

Gene position: 604785-605873 (Clockwise)

Preceding gene: 15607141

Following gene: 15606219

Centisome position: 38.98

GC content: 44.44

Gene sequence:

>1089_bases
ATGCTGAAGGAAGCCTACATATCCAGACTTGACAAACTCCAGGAAAAGTACAGGAAACTTCAGGAAGAACTCAGTAAACC
CGAAGTAATACAGGACGTAGAAAAGTACAAGAAACTCAGTAAAGAGCTTAAAGAACTCCAGGAGATAAACGAGCTATACG
AAAGATACAAAAAAGCCCAGAAGGAATTAAAGGAAGCAAAGGAGCTTTTGAAGAGTTCGGACAAGGACTTAAGGGAACTC
GCGGAAGAAGAGGTAAACAGACTTACTGAAGAGATGAAGAAACTTGAAGAAGAGTTAAAGGTTCACCTGGTTCCAAAGGA
TCCAAACGATACTAAAAACGTTATCCTTGAAATAAGGGCGGGAGCGGGTGGTGAGGAGGCTGCCCTCTTTGCCGCGGACC
TCTTCAGGATGTACCAGAAGTACGCAGAAGAAAAGGGCTGGAAGGTAAGTATACTCTCTTCAAACAAAACGGGACTCGGC
GGATACAAGGAAGTAATAGCCCTTATAGAAGGTGAAGGTGCTTATTCGAGGCTAAAGTACGAAAGCGGTGTCCACAGGGT
TCAAAGAGTACCTGTCACCGAAAGTAGCGGAAGGATTCACACCTCAACCGCCACCGTGGCGGTTCTTCCCGAAGTTGACG
AAACGGACATAAAGATTAAGCCAGAGGAACTGAAAATAGAAACCTTCAGGGCTTCGGGAGCGGGCGGACAGTACGTGAAC
ACCACCGAAACAGCTGTAAGGATTACACACATACCTACGGGTATAGTGGTTCAGTGCCAGGATGAAAGGTCTCAATTCCA
GAACAAACAAAAAGCTCTTAAAATCCTGTACGCGAAACTAAAGGACTACTACGAAAGAAAGAAGCAGGAAGAAATAGCGA
AGGAAAGAAAGGAACAGGTCGGAACTGGAGAGAGGAGTGAAAAGATAAGAACTTACAACTTCCCTCAAAATAGGGTTACC
GATCACAGGATAAACCTAACCCTTTACAAGCTTCAGGATGTTCTGGAAGGAAAACTGGACGAAATAATAGACGCCTTGAG
GGCGAAGGAAATAGAAAAGAAACTGGAACTAGTAGAAAAGGAAGGTTAA

Upstream 100 bases:

>100_bases
CAACCAGTGTCACCCCTTTTACGCGGGCAAACTGAAGATAAAGCCCGCTTACTACGAAATGCTTGCGGAATTTGAAGGAG
GTAAGAAAGAAGAGTAAAAA

Downstream 100 bases:

>100_bases
TCCTTTATAGGGATACTTATCCAGAGTTGTTCCTTGTCTACATCCTCCCTTTTGAGGTTAACTTCTATGTTGCTGGCGTC
AAAGTAGGGGTACTTTCCAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MLKEAYISRLDKLQEKYRKLQEELSKPEVIQDVEKYKKLSKELKELQEINELYERYKKAQKELKEAKELLKSSDKDLREL
AEEEVNRLTEEMKKLEEELKVHLVPKDPNDTKNVILEIRAGAGGEEAALFAADLFRMYQKYAEEKGWKVSILSSNKTGLG
GYKEVIALIEGEGAYSRLKYESGVHRVQRVPVTESSGRIHTSTATVAVLPEVDETDIKIKPEELKIETFRASGAGGQYVN
TTETAVRITHIPTGIVVQCQDERSQFQNKQKALKILYAKLKDYYERKKQEEIAKERKEQVGTGERSEKIRTYNFPQNRVT
DHRINLTLYKLQDVLEGKLDEIIDALRAKEIEKKLELVEKEG

Sequences:

>Translated_362_residues
MLKEAYISRLDKLQEKYRKLQEELSKPEVIQDVEKYKKLSKELKELQEINELYERYKKAQKELKEAKELLKSSDKDLREL
AEEEVNRLTEEMKKLEEELKVHLVPKDPNDTKNVILEIRAGAGGEEAALFAADLFRMYQKYAEEKGWKVSILSSNKTGLG
GYKEVIALIEGEGAYSRLKYESGVHRVQRVPVTESSGRIHTSTATVAVLPEVDETDIKIKPEELKIETFRASGAGGQYVN
TTETAVRITHIPTGIVVQCQDERSQFQNKQKALKILYAKLKDYYERKKQEEIAKERKEQVGTGERSEKIRTYNFPQNRVT
DHRINLTLYKLQDVLEGKLDEIIDALRAKEIEKKLELVEKEG
>Mature_362_residues
MLKEAYISRLDKLQEKYRKLQEELSKPEVIQDVEKYKKLSKELKELQEINELYERYKKAQKELKEAKELLKSSDKDLREL
AEEEVNRLTEEMKKLEEELKVHLVPKDPNDTKNVILEIRAGAGGEEAALFAADLFRMYQKYAEEKGWKVSILSSNKTGLG
GYKEVIALIEGEGAYSRLKYESGVHRVQRVPVTESSGRIHTSTATVAVLPEVDETDIKIKPEELKIETFRASGAGGQYVN
TTETAVRITHIPTGIVVQCQDERSQFQNKQKALKILYAKLKDYYERKKQEEIAKERKEQVGTGERSEKIRTYNFPQNRVT
DHRINLTLYKLQDVLEGKLDEIIDALRAKEIEKKLELVEKEG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=293, Percent_Identity=50.5119453924915, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI34577120, Length=348, Percent_Identity=40.8045977011494, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=48.9690721649485, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=50.5780346820809, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=37.8299120234604, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=42.9577464788732, Blast_Score=221, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6321295, Length=337, Percent_Identity=45.6973293768546, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=41.4110429447853, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_AQUAE (O67032)

Other databases:

- EMBL:   AE000657
- PIR:   E70375
- RefSeq:   NP_213594.1
- ProteinModelPortal:   O67032
- GeneID:   1193574
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_876
- NMPDR:   fig|224324.1.peg.609
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   O67032
- ProtClustDB:   PRK00591
- BioCyc:   AAEO224324:AQ_876-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41834; Mature: 41834

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEAYISRLDKLQEKYRKLQEELSKPEVIQDVEKYKKLSKELKELQEINELYERYKKAQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELKEAKELLKSSDKDLRELAEEEVNRLTEEMKKLEEELKVHLVPKDPNDTKNVILEIRA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEEC
GAGGEEAALFAADLFRMYQKYAEEKGWKVSILSSNKTGLGGYKEVIALIEGEGAYSRLKY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHH
ESGVHRVQRVPVTESSGRIHTSTATVAVLPEVDETDIKIKPEELKIETFRASGAGGQYVN
HHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCEECHHHEEEEEEEECCCCCCCCC
TTETAVRITHIPTGIVVQCQDERSQFQNKQKALKILYAKLKDYYERKKQEEIAKERKEQV
CHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTGERSEKIRTYNFPQNRVTDHRINLTLYKLQDVLEGKLDEIIDALRAKEIEKKLELVEK
CCCCCCCCCEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EG
CC
>Mature Secondary Structure
MLKEAYISRLDKLQEKYRKLQEELSKPEVIQDVEKYKKLSKELKELQEINELYERYKKAQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELKEAKELLKSSDKDLRELAEEEVNRLTEEMKKLEEELKVHLVPKDPNDTKNVILEIRA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEEC
GAGGEEAALFAADLFRMYQKYAEEKGWKVSILSSNKTGLGGYKEVIALIEGEGAYSRLKY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHH
ESGVHRVQRVPVTESSGRIHTSTATVAVLPEVDETDIKIKPEELKIETFRASGAGGQYVN
HHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCCCEECHHHEEEEEEEECCCCCCCCC
TTETAVRITHIPTGIVVQCQDERSQFQNKQKALKILYAKLKDYYERKKQEEIAKERKEQV
CHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTGERSEKIRTYNFPQNRVTDHRINLTLYKLQDVLEGKLDEIIDALRAKEIEKKLELVEK
CCCCCCCCCEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320