Click here to switch to the map view.

The map label for this gene is exsB

Identifier: 15606187

GI number: 15606187

Start: 573050

End: 573706

Strand: Direct

Name: exsB

Synonym: aq_831

Alternate gene names: 15606187

Gene position: 573050-573706 (Clockwise)

Preceding gene: 15606184

Following gene: 15606191

Centisome position: 36.94

GC content: 44.14

Gene sequence:

>657_bases
ATGAAAAAACACGATGGAATAATTGTCCTGCTTTCAGGCGGAATGGACAGTGCAACGTTACTTTGGCTTGCAAAAAGGGA
GTTTAAAAAGGTATACGCTATTTCCTTTGATTACGGTCAAAGGCATAAAGTTGAACTTAAATACGCAAAGGAACTTGCTA
AACTTGCAGAGGTGGAAGATCACTTTATAGTTCAGGTGCCGTTTTATACCTCGCTTAAAGGCTCTGCTTTAATAGACGAG
AGTGTTGAAGTTCCGAAAGGAGAGTACCCTGAAAACGAACCTCCTGTGACCACCGTTCCCATGAGGAATTTGATTTTTCT
CTCAATTGCCTCCGCTTTTGCGGACAACCTAGAGGTAAATTACATAGGGATAGGTGTTCACGCCCTTGATACACCTTATC
CAGATTGCAGACCTGAGTTTATAACCGCTGCGGAAGCAGCCATTAACGCAGGTTCTACTTTTGTTGCAAAGAAAAAGGAG
AGAATGCACGTTTACGCTCCCTTTTTGGGTATGAGTAAAAGGGATATAGCACTCCTTGGGAAGGAACTTGGTGTTCCCTT
TGAGAAGACTTACTCCTGCTATATGGGAACTGAACCACCCTGCGGAGAGTGTCCCACGTGTATACAGCGGGAGGAAGCTC
TAAGGGGAATCCTCTAA

Upstream 100 bases:

>100_bases
GTCAAGACTTTTTAGCTGCCTTTCTTCAGGTTAATCACCTGTGACATCTCCTTATTCTATATCCACTCCTACCCCTAGTC
AAGAGGTAAATTAATTTCCT

Downstream 100 bases:

>100_bases
GTACTTTATGCTTTTCATTACTTTGTAATTTGTAAGGTCAAGGTGCAGGGGTGTGACGGAAACGTATCCATTCAAAACTG
CCCAGTAGTCTGTCCCTTCT

Product: trans-regulatory protein ExsB

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKKHDGIIVLLSGGMDSATLLWLAKREFKKVYAISFDYGQRHKVELKYAKELAKLAEVEDHFIVQVPFYTSLKGSALIDE
SVEVPKGEYPENEPPVTTVPMRNLIFLSIASAFADNLEVNYIGIGVHALDTPYPDCRPEFITAAEAAINAGSTFVAKKKE
RMHVYAPFLGMSKRDIALLGKELGVPFEKTYSCYMGTEPPCGECPTCIQREEALRGIL

Sequences:

>Translated_218_residues
MKKHDGIIVLLSGGMDSATLLWLAKREFKKVYAISFDYGQRHKVELKYAKELAKLAEVEDHFIVQVPFYTSLKGSALIDE
SVEVPKGEYPENEPPVTTVPMRNLIFLSIASAFADNLEVNYIGIGVHALDTPYPDCRPEFITAAEAAINAGSTFVAKKKE
RMHVYAPFLGMSKRDIALLGKELGVPFEKTYSCYMGTEPPCGECPTCIQREEALRGIL
>Mature_218_residues
MKKHDGIIVLLSGGMDSATLLWLAKREFKKVYAISFDYGQRHKVELKYAKELAKLAEVEDHFIVQVPFYTSLKGSALIDE
SVEVPKGEYPENEPPVTTVPMRNLIFLSIASAFADNLEVNYIGIGVHALDTPYPDCRPEFITAAEAAINAGSTFVAKKKE
RMHVYAPFLGMSKRDIALLGKELGVPFEKTYSCYMGTEPPCGECPTCIQREEALRGIL

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=216, Percent_Identity=32.8703703703704, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_AQUAE (O67003)

Other databases:

- EMBL:   AE000657
- PIR:   H70371
- RefSeq:   NP_213564.1
- ProteinModelPortal:   O67003
- SMR:   O67003
- GeneID:   1193453
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_831
- NMPDR:   fig|224324.1.peg.579
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- PhylomeDB:   O67003
- ProtClustDB:   CLSK2299513
- BioCyc:   AAEO224324:AQ_831-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24238; Mature: 24238

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHDGIIVLLSGGMDSATLLWLAKREFKKVYAISFDYGQRHKVELKYAKELAKLAEVED
CCCCCCEEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCCCEEEEHHHHHHHHHHHCCCC
HFIVQVPFYTSLKGSALIDESVEVPKGEYPENEPPVTTVPMRNLIFLSIASAFADNLEVN
CEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCEEE
YIGIGVHALDTPYPDCRPEFITAAEAAINAGSTFVAKKKERMHVYAPFLGMSKRDIALLG
EEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCHHHHHH
KELGVPFEKTYSCYMGTEPPCGECPTCIQREEALRGIL
HHHCCCHHHCEEEECCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKHDGIIVLLSGGMDSATLLWLAKREFKKVYAISFDYGQRHKVELKYAKELAKLAEVED
CCCCCCEEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCCCEEEEHHHHHHHHHHHCCCC
HFIVQVPFYTSLKGSALIDESVEVPKGEYPENEPPVTTVPMRNLIFLSIASAFADNLEVN
CEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCEEE
YIGIGVHALDTPYPDCRPEFITAAEAAINAGSTFVAKKKERMHVYAPFLGMSKRDIALLG
EEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCHHHHHH
KELGVPFEKTYSCYMGTEPPCGECPTCIQREEALRGIL
HHHCCCHHHCEEEECCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320