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The map label for this gene is nifS2 [H]

Identifier: 15606131

GI number: 15606131

Start: 512737

End: 513957

Strand: Direct

Name: nifS2 [H]

Synonym: aq_739

Alternate gene names: 15606131

Gene position: 512737-513957 (Clockwise)

Preceding gene: 15606130

Following gene: 15606132

Centisome position: 33.05

GC content: 47.91

Gene sequence:

>1221_bases
ATGTTCAGAACGAAAGCCGGCAAAAAAGTAGTTTACGTTGACCATATAGCTACAACACCGGTAGCGGAAGAAGTTCTTGA
AGCTATGCTCCCTTACTTCAGGGAGAAGTTCGGTAATCCCACATCTCTCCACAGCTTCGGACAGGAGGCAAAGAAAGCGG
TAGAAAAAGCCAGAGAACAAGTTGCACAGCTCATAAACGCAAACATCCCCGAAGAGATCATATTCACATCCGGAGGAATA
GAGGCTAATAACCTTGCGATAAAAGGTATAGCAAAGGCTTACCAGAGGAGAGGAAAGCACATAGTTACCACGGAAATAGA
GCACCACTCCATCCTTCACCCTTGTAAAACCCTAGAAAGAGAAGGCTGGGAAGTTACGTACTTAAAACCCGATAAGTACG
GCTTAATAGACCCAGAGCAAGTTAGAGAAGCGGTAAGGGAAGACACCGTCCTCGTTTCCATAGGGCATTCCAACAGGGAA
ATAGGAACTATTCAAAACATAAAGGAACTCGTAAAGGCGGCAAAGGAAAAGAACCCCAAAGTTATATTCCACACGGACGC
CGCACCTTCTCTCGGACACTACCCCGTTGACGTTCAGGACTGGGGAGTAGACGCTGCTTCTTTTACCGCACACCTCATGT
ACGGTCCCAAGGGAGTGGGAGCACTATGGACCAGAAAGGGCGTTAAGGTTAAGCCCCTGATAGAAGGTGGAACCCAGGAA
AGAGGAGTGAGGGCGGGAACGGAAAACGTCCCCGGAATAGTTGGTTTCGGAGCCGCAGCAGAACTTGCCATGAAGGAACT
CGACGACAGGATGAAGAGGCTCTCCCACTACAGGGATAAACTCAGAAAGGGACTGGAAGAAAAGGTGGATTACATAGAGT
TTACAGGACATCCAACTCAAAGACTTCCCCACCACCTCTCAATAATCGTACACTTCGTGGAAGGTGAGGCTATGCTCCTG
AGACTTGACCTCATGGGCATAGAAACTGCTTCAGGTTCTGCGTGCGTATCCTTGGCACTCAAGCAGTCACACGTTCTCAC
AGCTATAGGTATCCCTAAAGAAGTTTCAAACGGTTCGGTAGTGTTCTCCTTCGGTAGGGAAAATACGGAAGAAGATGTGG
ACTACATACTCGAAGAGTTCCCCAAAGTTATCAACTGGCTCAGGGAAGTTTCTCCCTTCAATCCAGAGAACTGGGAAAAG
TACGTGAAATCCAGAGGATAA

Upstream 100 bases:

>100_bases
ACCCCGACTCGGAAGTTGCAAAGGCATTCCTTAGCATAGCCAAAGTTTTGAGCCAGGTTGTAGAAAGTAAAGTAAATTAA
TCTAAAAAGGAGGTTGTAAA

Downstream 100 bases:

>100_bases
AATAAAACTTTATGAACAAACTCTCCCTTTTCGTTTATCTATTTTTCTTCCTTTCCTTTCTATTTCTGTTTTTATACCTC
CTCCAGCCCTTTTTTAACCC

Product: FeS cluster formation protein NifS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFRTKAGKKVVYVDHIATTPVAEEVLEAMLPYFREKFGNPTSLHSFGQEAKKAVEKAREQVAQLINANIPEEIIFTSGGI
EANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTLEREGWEVTYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNRE
IGTIQNIKELVKAAKEKNPKVIFHTDAAPSLGHYPVDVQDWGVDAASFTAHLMYGPKGVGALWTRKGVKVKPLIEGGTQE
RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEKVDYIEFTGHPTQRLPHHLSIIVHFVEGEAMLL
RLDLMGIETASGSACVSLALKQSHVLTAIGIPKEVSNGSVVFSFGRENTEEDVDYILEEFPKVINWLREVSPFNPENWEK
YVKSRG

Sequences:

>Translated_406_residues
MFRTKAGKKVVYVDHIATTPVAEEVLEAMLPYFREKFGNPTSLHSFGQEAKKAVEKAREQVAQLINANIPEEIIFTSGGI
EANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTLEREGWEVTYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNRE
IGTIQNIKELVKAAKEKNPKVIFHTDAAPSLGHYPVDVQDWGVDAASFTAHLMYGPKGVGALWTRKGVKVKPLIEGGTQE
RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEKVDYIEFTGHPTQRLPHHLSIIVHFVEGEAMLL
RLDLMGIETASGSACVSLALKQSHVLTAIGIPKEVSNGSVVFSFGRENTEEDVDYILEEFPKVINWLREVSPFNPENWEK
YVKSRG
>Mature_406_residues
MFRTKAGKKVVYVDHIATTPVAEEVLEAMLPYFREKFGNPTSLHSFGQEAKKAVEKAREQVAQLINANIPEEIIFTSGGI
EANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTLEREGWEVTYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNRE
IGTIQNIKELVKAAKEKNPKVIFHTDAAPSLGHYPVDVQDWGVDAASFTAHLMYGPKGVGALWTRKGVKVKPLIEGGTQE
RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEKVDYIEFTGHPTQRLPHHLSIIVHFVEGEAMLL
RLDLMGIETASGSACVSLALKQSHVLTAIGIPKEVSNGSVVFSFGRENTEEDVDYILEEFPKVINWLREVSPFNPENWEK
YVKSRG

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=43.6224489795918, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI156713448, Length=421, Percent_Identity=33.4916864608076, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI48994898, Length=396, Percent_Identity=42.4242424242424, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1787970, Length=379, Percent_Identity=25.3298153034301, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=25, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=393, Percent_Identity=41.9847328244275, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17533177, Length=319, Percent_Identity=33.2288401253918, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=42.6065162907268, Blast_Score=317, Evalue=2e-87,
Organism=Drosophila melanogaster, GI20129463, Length=392, Percent_Identity=42.8571428571429, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45316; Mature: 45316

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTKAGKKVVYVDHIATTPVAEEVLEAMLPYFREKFGNPTSLHSFGQEAKKAVEKAREQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VAQLINANIPEEIIFTSGGIEANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTLER
HHHHHCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHC
EGWEVTYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNREIGTIQNIKELVKAAKEKNPK
CCCEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VIFHTDAAPSLGHYPVDVQDWGVDAASFTAHLMYGPKGVGALWTRKGVKVKPLIEGGTQE
EEEECCCCCCCCCCCCCHHHCCCCHHHHHEEEEECCCCCCHHHHCCCCEEEEEECCCCCC
RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEKVDYIEFTGHPTQ
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHH
RLPHHLSIIVHFVEGEAMLLRLDLMGIETASGSACVSLALKQSHVLTAIGIPKEVSNGSV
HCCHHEEHHEEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCE
VFSFGRENTEEDVDYILEEFPKVINWLREVSPFNPENWEKYVKSRG
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MFRTKAGKKVVYVDHIATTPVAEEVLEAMLPYFREKFGNPTSLHSFGQEAKKAVEKAREQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VAQLINANIPEEIIFTSGGIEANNLAIKGIAKAYQRRGKHIVTTEIEHHSILHPCKTLER
HHHHHCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHC
EGWEVTYLKPDKYGLIDPEQVREAVREDTVLVSIGHSNREIGTIQNIKELVKAAKEKNPK
CCCEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VIFHTDAAPSLGHYPVDVQDWGVDAASFTAHLMYGPKGVGALWTRKGVKVKPLIEGGTQE
EEEECCCCCCCCCCCCCHHHCCCCHHHHHEEEEECCCCCCHHHHCCCCEEEEEECCCCCC
RGVRAGTENVPGIVGFGAAAELAMKELDDRMKRLSHYRDKLRKGLEEKVDYIEFTGHPTQ
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHH
RLPHHLSIIVHFVEGEAMLLRLDLMGIETASGSACVSLALKQSHVLTAIGIPKEVSNGSV
HCCHHEEHHEEEECCCEEEEEEEEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCE
VFSFGRENTEEDVDYILEEFPKVINWLREVSPFNPENWEKYVKSRG
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]