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The map label for this gene is kpsU

Identifier: 15606097

GI number: 15606097

Start: 470797

End: 471501

Strand: Direct

Name: kpsU

Synonym: aq_692

Alternate gene names: 15606097

Gene position: 470797-471501 (Clockwise)

Preceding gene: 15606094

Following gene: 15606106

Centisome position: 30.35

GC content: 41.56

Gene sequence:

>705_bases
ATGAGAAGAGCTGTAATCATACCTGCAAGACTAGGCTCAACAAGGCTAAAGGAAAAACCTTTAAAAAATTTACTCGGAAA
GCCCCTCATAAGGTGGGTAGTTGAAGGACTCGTAAAAACGGGAGAGAGGGTAATCCTCGCTACGGACAGCGAAAGGGTAA
AGGAAGTGGTTGAGGACCTATGTGAAGTTTTCCTTACCCCCTCGGACCTGCCCTCGGGCAGTGACAGAGTTCTGTACGTC
GTTAGAGATTTGGATGTTGACTTAATTATCAATTATCAGGGAGATGAGCCCTTTGTTTACGAGGAAGATATAAAATTGAT
ATTTAGAGAACTTGAAAAGGGAGAAAGAGTTGTAACCCTTGCAAGGAAGGACAAGGAGGCTTACGAGAGACCTGAAGACG
TGAAGGTGGTTCTGGACAGGGAAGGATACGCCCTTTACTTTTCCCGCTCACCTATCCCATACTTTAGGAAAAATGATACA
TTTTATCCTTTAAAACACGTAGGGATTTACGGCTTCAGAAAGGAAACTTTAATGGAGTTCGGCGCTATGCCTCCCTCAAA
ACTTGAACAAATAGAAGGACTTGAACAGTTAAGGCTTTTAGAGAACGGAATAAAGATAAAGGTTTTAATTACGGAGAATT
ACTACCACGGAGTTGATACGGAGGAGGACTTAAAGATAGTGGAAGAAAAGTTAAAAAATTTATAA

Upstream 100 bases:

>100_bases
TTTGCGAGTCTCCACGCTGCTTCTGAGCCAGCAAGCCCTCCTCCGATTACTATAACCTTTTCCTTCATTGCTTTTATGAA
TTTAAACTAAAGTTTTGTAT

Downstream 100 bases:

>100_bases
AAAAAGGAAAGCTCCTCGCAGGGGCAAAAAGGAACTGTCAGAAGTAAAACTGCATCTGTATCTGGTGAACCGTTTGGTCC
TTTTCGTCTTTTTCTTTGTT

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRRAVIIPARLGSTRLKEKPLKNLLGKPLIRWVVEGLVKTGERVILATDSERVKEVVEDLCEVFLTPSDLPSGSDRVLYV
VRDLDVDLIINYQGDEPFVYEEDIKLIFRELEKGERVVTLARKDKEAYERPEDVKVVLDREGYALYFSRSPIPYFRKNDT
FYPLKHVGIYGFRKETLMEFGAMPPSKLEQIEGLEQLRLLENGIKIKVLITENYYHGVDTEEDLKIVEEKLKNL

Sequences:

>Translated_234_residues
MRRAVIIPARLGSTRLKEKPLKNLLGKPLIRWVVEGLVKTGERVILATDSERVKEVVEDLCEVFLTPSDLPSGSDRVLYV
VRDLDVDLIINYQGDEPFVYEEDIKLIFRELEKGERVVTLARKDKEAYERPEDVKVVLDREGYALYFSRSPIPYFRKNDT
FYPLKHVGIYGFRKETLMEFGAMPPSKLEQIEGLEQLRLLENGIKIKVLITENYYHGVDTEEDLKIVEEKLKNL
>Mature_234_residues
MRRAVIIPARLGSTRLKEKPLKNLLGKPLIRWVVEGLVKTGERVILATDSERVKEVVEDLCEVFLTPSDLPSGSDRVLYV
VRDLDVDLIINYQGDEPFVYEEDIKLIFRELEKGERVVTLARKDKEAYERPEDVKVVLDREGYALYFSRSPIPYFRKNDT
FYPLKHVGIYGFRKETLMEFGAMPPSKLEQIEGLEQLRLLENGIKIKVLITENYYHGVDTEEDLKIVEEKLKNL

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=250, Percent_Identity=41.6, Blast_Score=148, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_AQUAE (O66914)

Other databases:

- EMBL:   AE000657
- PIR:   F70360
- RefSeq:   NP_213474.1
- ProteinModelPortal:   O66914
- SMR:   O66914
- GeneID:   1193976
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_692
- NMPDR:   fig|224324.1.peg.489
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- PhylomeDB:   O66914
- ProtClustDB:   PRK05450
- BioCyc:   AAEO224324:AQ_692-MONOMER
- BRENDA:   2.7.7.38
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27174; Mature: 27174

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAVIIPARLGSTRLKEKPLKNLLGKPLIRWVVEGLVKTGERVILATDSERVKEVVEDL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
CEVFLTPSDLPSGSDRVLYVVRDLDVDLIINYQGDEPFVYEEDIKLIFRELEKGERVVTL
HHHHCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCEEEHHHHHHHHHHHHCCCEEEEE
ARKDKEAYERPEDVKVVLDREGYALYFSRSPIPYFRKNDTFYPLKHVGIYGFRKETLMEF
EHHCHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH
GAMPPSKLEQIEGLEQLRLLENGIKIKVLITENYYHGVDTEEDLKIVEEKLKNL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRAVIIPARLGSTRLKEKPLKNLLGKPLIRWVVEGLVKTGERVILATDSERVKEVVEDL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
CEVFLTPSDLPSGSDRVLYVVRDLDVDLIINYQGDEPFVYEEDIKLIFRELEKGERVVTL
HHHHCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCEEEHHHHHHHHHHHHCCCEEEEE
ARKDKEAYERPEDVKVVLDREGYALYFSRSPIPYFRKNDTFYPLKHVGIYGFRKETLMEF
EHHCHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHH
GAMPPSKLEQIEGLEQLRLLENGIKIKVLITENYYHGVDTEEDLKIVEEKLKNL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320