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The map label for this gene is glmU

Identifier: 15606046

GI number: 15606046

Start: 412805

End: 414199

Strand: Direct

Name: glmU

Synonym: aq_607

Alternate gene names: 15606046

Gene position: 412805-414199 (Clockwise)

Preceding gene: 15606045

Following gene: 15606050

Centisome position: 26.61

GC content: 45.3

Gene sequence:

>1395_bases
ATGCGTGCTGTAATTCTGGCTGCCGGACTCGGAACGAGGTTCAAGAGCGAAAAACCCAAAGTTCTCCACGAAATACTCGG
AAAACCGATGCTCTGGTACGTGATAACGAACGTAAGAAACGGAAGGATAGACGACATAGCTGTCGTGGTGGGACACAAGG
CGCAGGAAGTGATGGAAGCCTTTAAAAACGAAAACCTGAAATTTTTTATACAGGAAAACCCCAAAGGCGGGACTGCTGAC
GCTGTACTCGCAGCGAAGGACTTTTTCTCTTCTTACGAGGGATACGTACTCATAATAAACGGGGACAGTCCCTTAGTCAG
CGGGGAAACTATAAGAAACATGCAACAATTCATTCACATGGTTCGGACTTACGAGGGGATAAAACTCGGAGGGGTTGTTT
TAACGACCCACCTTCCCGACCCTACTGGATACGGGAGGATTATAAAGGAAGAGGGGACGGACAGAATTATCAGGATAGTT
GAGGAAAAGGACGCAACTCCCGAAGAAAAGGCTATAACGGAAATAAACGCTGGAACTTACATCTTTTACGCACCTTACCT
CCTTGAAGCCCTATACAGGATAAAACCGAGTCCCGTGACAGGAGAGCTCTACCTGACCGACGTAATAGAGTACATGGTAA
ACAAAGGGTATGAAGTAAGGAGCTTTATGGCAAAAGAACCCACGGAAGCCCTTGGTGTAAACACCCGCTGGGATTTAGCA
CTCGTAGAAAACGTAATAAAACTCAAGATAGCGAGGTACTGGGCCGAAAGGGGAGTTACTGTTCATTATCCAGAAACCGT
GTGGATAGAACCGGACGTTTCCATAGAACCCGACGTGGAAATATTTCCCGACGTTATGTTGAAAGGAAAGACGAAAATAA
AGAAAGGTTCAGTAATAGGAAAGGGAAGCGTTATAAAGGACTCACTCGTGGAAGAAAATGTAATAGTAAGGGAATACTCG
GTAATAGAAAATTCGGAAATTAAGAAAAGGGCTGTTGTCGGACCCTTTGCGAGGATAAGGAACGAATCTGTTATAGGAGA
AGAGGCTGAAATAGGGAACTTTGTGGAAGTTAAGAAGAGCAGTATAGGAAAAGGGGTAAAGGCAAAGCACCTCGCTTACA
TAGGGGATGCAACTGTGGGAGAGAATACGAATATAGGGGCGGGTACGGTCTTTGCAAATTACGACGGGAAGAGGAAATAC
GAGAGCTACGTAGGAAAAAGCGCCTTTATAGGTAGTAACTCCCTTTTAATAGCTCCTATAAGGGTAGGGGATTGGGCTTA
TATAGCGGGGGGTTCCGTTGTAAACAAAGATATACCGGAAGGGGCACTCGCGGTCTCAAGGCCTGAACTGAAAATTTTTG
AAGGAAGGGGGAAGAAAAAACTCCAGAAAGATTAA

Upstream 100 bases:

>100_bases
GAACTTGGACAGTACGAGGAAGTGAGGAAAATGGTTGAGTTTATAAAGAGTTCCAAAAGAGGAGTGGCGAGACACCACAA
GGACTGATAACATAACATTC

Downstream 100 bases:

>100_bases
ACTCTTGCAAGAACTTTTTCTTTGAAGTCTTTTAACGTCGGTTTTATCTTCACAGGTGTGCTTAGTTTCCCTTCCAGAAC
TTCCATAGTTTTATAGCCGT

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRAVILAAGLGTRFKSEKPKVLHEILGKPMLWYVITNVRNGRIDDIAVVVGHKAQEVMEAFKNENLKFFIQENPKGGTAD
AVLAAKDFFSSYEGYVLIINGDSPLVSGETIRNMQQFIHMVRTYEGIKLGGVVLTTHLPDPTGYGRIIKEEGTDRIIRIV
EEKDATPEEKAITEINAGTYIFYAPYLLEALYRIKPSPVTGELYLTDVIEYMVNKGYEVRSFMAKEPTEALGVNTRWDLA
LVENVIKLKIARYWAERGVTVHYPETVWIEPDVSIEPDVEIFPDVMLKGKTKIKKGSVIGKGSVIKDSLVEENVIVREYS
VIENSEIKKRAVVGPFARIRNESVIGEEAEIGNFVEVKKSSIGKGVKAKHLAYIGDATVGENTNIGAGTVFANYDGKRKY
ESYVGKSAFIGSNSLLIAPIRVGDWAYIAGGSVVNKDIPEGALAVSRPELKIFEGRGKKKLQKD

Sequences:

>Translated_464_residues
MRAVILAAGLGTRFKSEKPKVLHEILGKPMLWYVITNVRNGRIDDIAVVVGHKAQEVMEAFKNENLKFFIQENPKGGTAD
AVLAAKDFFSSYEGYVLIINGDSPLVSGETIRNMQQFIHMVRTYEGIKLGGVVLTTHLPDPTGYGRIIKEEGTDRIIRIV
EEKDATPEEKAITEINAGTYIFYAPYLLEALYRIKPSPVTGELYLTDVIEYMVNKGYEVRSFMAKEPTEALGVNTRWDLA
LVENVIKLKIARYWAERGVTVHYPETVWIEPDVSIEPDVEIFPDVMLKGKTKIKKGSVIGKGSVIKDSLVEENVIVREYS
VIENSEIKKRAVVGPFARIRNESVIGEEAEIGNFVEVKKSSIGKGVKAKHLAYIGDATVGENTNIGAGTVFANYDGKRKY
ESYVGKSAFIGSNSLLIAPIRVGDWAYIAGGSVVNKDIPEGALAVSRPELKIFEGRGKKKLQKD
>Mature_464_residues
MRAVILAAGLGTRFKSEKPKVLHEILGKPMLWYVITNVRNGRIDDIAVVVGHKAQEVMEAFKNENLKFFIQENPKGGTAD
AVLAAKDFFSSYEGYVLIINGDSPLVSGETIRNMQQFIHMVRTYEGIKLGGVVLTTHLPDPTGYGRIIKEEGTDRIIRIV
EEKDATPEEKAITEINAGTYIFYAPYLLEALYRIKPSPVTGELYLTDVIEYMVNKGYEVRSFMAKEPTEALGVNTRWDLA
LVENVIKLKIARYWAERGVTVHYPETVWIEPDVSIEPDVEIFPDVMLKGKTKIKKGSVIGKGSVIKDSLVEENVIVREYS
VIENSEIKKRAVVGPFARIRNESVIGEEAEIGNFVEVKKSSIGKGVKAKHLAYIGDATVGENTNIGAGTVFANYDGKRKY
ESYVGKSAFIGSNSLLIAPIRVGDWAYIAGGSVVNKDIPEGALAVSRPELKIFEGRGKKKLQKD

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Homo sapiens, GI11761621, Length=376, Percent_Identity=25, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI11761619, Length=353, Percent_Identity=24.929178470255, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1790168, Length=447, Percent_Identity=38.0313199105145, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI133931050, Length=382, Percent_Identity=23.0366492146597, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355443, Length=367, Percent_Identity=23.9782016348774, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644084, Length=367, Percent_Identity=23.9782016348774, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_AQUAE (O66863)

Other databases:

- EMBL:   AE000657
- PIR:   C70354
- RefSeq:   NP_213423.1
- ProteinModelPortal:   O66863
- SMR:   O66863
- GeneID:   1193925
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_607
- NMPDR:   fig|224324.1.peg.438
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- PhylomeDB:   O66863
- ProtClustDB:   CLSK2299448
- BioCyc:   AAEO224324:AQ_607-MONOMER
- BRENDA:   2.3.1.157
- BRENDA:   2.7.7.23
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 51421; Mature: 51421

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 370-370 BINDING 145-145 BINDING 161-161 BINDING 176-176 BINDING 394-394 BINDING 412-412 BINDING 430-430 BINDING 447-447

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVILAAGLGTRFKSEKPKVLHEILGKPMLWYVITNVRNGRIDDIAVVVGHKAQEVMEA
CCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHH
FKNENLKFFIQENPKGGTADAVLAAKDFFSSYEGYVLIINGDSPLVSGETIRNMQQFIHM
HHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
VRTYEGIKLGGVVLTTHLPDPTGYGRIIKEEGTDRIIRIVEEKDATPEEKAITEINAGTY
HHHHCCEEECCEEEEEECCCCCCCCCEEECCCCCCEEEEEECCCCCCCHHHHEEECCCEE
IFYAPYLLEALYRIKPSPVTGELYLTDVIEYMVNKGYEVRSFMAKEPTEALGVNTRWDLA
EEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCCCHHH
LVENVIKLKIARYWAERGVTVHYPETVWIEPDVSIEPDVEIFPDVMLKGKTKIKKGSVIG
HHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCHHCCHHHCCCCCEEECCCEEC
KGSVIKDSLVEENVIVREYSVIENSEIKKRAVVGPFARIRNESVIGEEAEIGNFVEVKKS
CCCCHHHHHHCCCEEEEEEHHHCCCCHHHHHHHCHHHHHCCCCCCCCCHHCCCHHEEHHH
SIGKGVKAKHLAYIGDATVGENTNIGAGTVFANYDGKRKYESYVGKSAFIGSNSLLIAPI
HCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEECCCCEEEEEE
RVGDWAYIAGGSVVNKDIPEGALAVSRPELKIFEGRGKKKLQKD
EECCEEEEECCCEECCCCCCCCEEECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRAVILAAGLGTRFKSEKPKVLHEILGKPMLWYVITNVRNGRIDDIAVVVGHKAQEVMEA
CCEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHH
FKNENLKFFIQENPKGGTADAVLAAKDFFSSYEGYVLIINGDSPLVSGETIRNMQQFIHM
HHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
VRTYEGIKLGGVVLTTHLPDPTGYGRIIKEEGTDRIIRIVEEKDATPEEKAITEINAGTY
HHHHCCEEECCEEEEEECCCCCCCCCEEECCCCCCEEEEEECCCCCCCHHHHEEECCCEE
IFYAPYLLEALYRIKPSPVTGELYLTDVIEYMVNKGYEVRSFMAKEPTEALGVNTRWDLA
EEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCCCHHH
LVENVIKLKIARYWAERGVTVHYPETVWIEPDVSIEPDVEIFPDVMLKGKTKIKKGSVIG
HHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCHHCCHHHCCCCCEEECCCEEC
KGSVIKDSLVEENVIVREYSVIENSEIKKRAVVGPFARIRNESVIGEEAEIGNFVEVKKS
CCCCHHHHHHCCCEEEEEEHHHCCCCHHHHHHHCHHHHHCCCCCCCCCHHCCCHHEEHHH
SIGKGVKAKHLAYIGDATVGENTNIGAGTVFANYDGKRKYESYVGKSAFIGSNSLLIAPI
HCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHCCCEEEECCCCEEEEEE
RVGDWAYIAGGSVVNKDIPEGALAVSRPELKIFEGRGKKKLQKD
EECCEEEEECCCEECCCCCCCCEEECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320