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The map label for this gene is spsK [H]

Identifier: 15605986

GI number: 15605986

Start: 351738

End: 352151

Strand: Direct

Name: spsK [H]

Synonym: aq_518

Alternate gene names: 15605986

Gene position: 351738-352151 (Clockwise)

Preceding gene: 15605985

Following gene: 15605987

Centisome position: 22.67

GC content: 36.47

Gene sequence:

>414_bases
ATGAATGGGAAGGCCGGTGCAAGCAGTAAAGGTGGAAATTTTGTGTATACAATAATTCGCAAAGCTAAGGCAGGAGAACC
GCTAAGGGTGGTTGATGATATATACATGTCTCCTACCTATACCTTAGATGCTGCAAAAGAGATATGGAAAATTTTACTTG
AAAATAAACCTTACGGTATATACCACGTTACAAACTCAGGATACTGCTCATGGTATGAGTTTGCTGTCAAGATACTCGAA
TACTCTGGATTGAAAACGGATATAAAACCTGTAAGACATACAGAATTTAAGACTAAAGCAAATAGACCACTGTGGAGCCC
TTTAGCAAGTAAAAGGGGAATAAAACTGAGAAACTGGGAAGAAGCTTTAAAGGATTTTATAAATGCTATTTCCATTAATA
ACCTTTCTTATTAG

Upstream 100 bases:

>100_bases
TAAAATATTAAATTTGTACGAGGAGGTTTTAAAATGATTATCCGATATCATGCTCCTTTGGGACTTGGACTTGCAGTGGT
GTAACTGATCTAAGTCCATT

Downstream 100 bases:

>100_bases
CTTCTTTCTCTGTTTTCTATTCATAAAGTACATTAACCTAAATCTCTTCCACGATACTCAAAAAGGTGTTCAGAAATTTC
ACCACAGGCCTACACCCAGA

Product: spore coat polysaccharide biosynthesis protein SpsK

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MNGKAGASSKGGNFVYTIIRKAKAGEPLRVVDDIYMSPTYTLDAAKEIWKILLENKPYGIYHVTNSGYCSWYEFAVKILE
YSGLKTDIKPVRHTEFKTKANRPLWSPLASKRGIKLRNWEEALKDFINAISINNLSY

Sequences:

>Translated_137_residues
MNGKAGASSKGGNFVYTIIRKAKAGEPLRVVDDIYMSPTYTLDAAKEIWKILLENKPYGIYHVTNSGYCSWYEFAVKILE
YSGLKTDIKPVRHTEFKTKANRPLWSPLASKRGIKLRNWEEALKDFINAISINNLSY
>Mature_137_residues
MNGKAGASSKGGNFVYTIIRKAKAGEPLRVVDDIYMSPTYTLDAAKEIWKILLENKPYGIYHVTNSGYCSWYEFAVKILE
YSGLKTDIKPVRHTEFKTKANRPLWSPLASKRGIKLRNWEEALKDFINAISINNLSY

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 15609; Mature: 15609

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKAGASSKGGNFVYTIIRKAKAGEPLRVVDDIYMSPTYTLDAAKEIWKILLENKPYGI
CCCCCCCCCCCCCEEEEEEEHHCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEE
YHVTNSGYCSWYEFAVKILEYSGLKTDIKPVRHTEFKTKANRPLWSPLASKRGIKLRNWE
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHH
EALKDFINAISINNLSY
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNGKAGASSKGGNFVYTIIRKAKAGEPLRVVDDIYMSPTYTLDAAKEIWKILLENKPYGI
CCCCCCCCCCCCCEEEEEEEHHCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEE
YHVTNSGYCSWYEFAVKILEYSGLKTDIKPVRHTEFKTKANRPLWSPLASKRGIKLRNWE
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHH
EALKDFINAISINNLSY
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]