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The map label for this gene is eno

Identifier: 15605961

GI number: 15605961

Start: 326762

End: 328042

Strand: Direct

Name: eno

Synonym: aq_484

Alternate gene names: 15605961

Gene position: 326762-328042 (Clockwise)

Preceding gene: 15605957

Following gene: 15605968

Centisome position: 21.06

GC content: 48.79

Gene sequence:

>1281_bases
ATGTCAAGGATAAAAAGGGTTCACGGAAGAGAAGTTCTTGACTCCAGAGGAAACCCTACCGTTGAGGTGGAGGTGGAATT
AGAGAGCGGAGCCCTCGGAAGGGCTATAGTCCCGAGCGGTGCTTCAACGGGTGAAAGGGAGGCTTTAGAGCTCAGGGACG
GAGACCCCAAAAGGTATCTGGGGAAGGGAGTTTTAAAGGCGGTTGACAACGTAAACGGAGTTATAGCAAAGGCACTGGTG
GGACTTGAGCCCTACAACCAGAGGGAGATAGACCAGATACTTATAGAACTTGACGGAACGGAGAATAAAAGCAAACTTGG
GGCGAACGCCATCCTCGGAACGAGCATGGCCGTGGCAAGGGCTGCGGCAAACGAACTGGGAATTCCCCTATACGAATACC
TCGGAGGGAAGTTCGGTTACAGACTTCCCGTTCCCTTGATGAACGTTATAAATGGTGGAGCCCACGCGGATAACAACCTA
GACATTCAGGAGTTCATGATCGTTCCGGTGTGCGGAGGAGCTTTCAGGGAAGCCCTGAGGGCAGGAGTGGAGACCTTCCA
CCATCTCAAGAAAATACTGAAGGAGAAGGGATACTCAACGAATGTGGGAGACGAGGGCGGATTTGCTCCGAACCTAAATT
CCTCCGAGGAAGCTTTAGATATACTAATGCAGGCTATAGAAAAGGCGGGCTACAAACCTGGTGAGGACATTCTCCTCGCA
CTTGACGTTGCATCTTCAGAGTTTTACGAAAACGGAGTTTACAAGTTTGAAGGAAAGGAAAGAAGTGCTGAAGAAATGAT
AGAGTTCTACGAAAAGTTAATACAGAAGTACCCGATAATTTCAATAGAAGACCCGATGAGTGAAAACGACTGGGAGGGCT
GGAAGGAGATAACAAAAAGACTCGGGGATAAAGTTCAGCTCGTGGGAGACGACCTCTTCACTACAAACCCGAAAATCTTG
AGGAAGGGTATAGAGGAAGGAGTTGCAAACGCAATCTTAGTGAAACTGAACCAGATAGGGACTGTTTCTGAAACCCTGGA
CACGGTAATGCTCGCAAAGGAAAGAAATTACTCAGCAATAATCTCCCACCGTTCCGGAGAAACAGAAGACACCTTCATCT
CTCACCTTGCGGTGGCGACGAACGCGGGTCAGATAAAAACGGGTTCTGCTTCCAGAACGGACAGGATAGCGAAGTACAAC
GAACTGTTAAGGATAGAGGAGAGACTGGGAAATGGAGCAGTCTTCTGGGGAAGGGAAGAGTTCTACAGGTTCACTTCCTA
A

Upstream 100 bases:

>100_bases
TTGCGAGTTTCCTTCTAAATGCGTTCTGTAATTTATAGAAATCCCTGTATTCCTCTCCTTTGAACCTTTCCAGGTACTTT
TCCATAGTAAGATAGTATAC

Downstream 100 bases:

>100_bases
GAAAATTCTCCAGACCTTTCTCTTCCTTCTCCTTATAAACACCTTTGTAAACCTGTTTTTAAGCAAACCGACGATTTTTG
ACTTAATAAAACTTAAAAAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSRIKRVHGREVLDSRGNPTVEVEVELESGALGRAIVPSGASTGEREALELRDGDPKRYLGKGVLKAVDNVNGVIAKALV
GLEPYNQREIDQILIELDGTENKSKLGANAILGTSMAVARAAANELGIPLYEYLGGKFGYRLPVPLMNVINGGAHADNNL
DIQEFMIVPVCGGAFREALRAGVETFHHLKKILKEKGYSTNVGDEGGFAPNLNSSEEALDILMQAIEKAGYKPGEDILLA
LDVASSEFYENGVYKFEGKERSAEEMIEFYEKLIQKYPIISIEDPMSENDWEGWKEITKRLGDKVQLVGDDLFTTNPKIL
RKGIEEGVANAILVKLNQIGTVSETLDTVMLAKERNYSAIISHRSGETEDTFISHLAVATNAGQIKTGSASRTDRIAKYN
ELLRIEERLGNGAVFWGREEFYRFTS

Sequences:

>Translated_426_residues
MSRIKRVHGREVLDSRGNPTVEVEVELESGALGRAIVPSGASTGEREALELRDGDPKRYLGKGVLKAVDNVNGVIAKALV
GLEPYNQREIDQILIELDGTENKSKLGANAILGTSMAVARAAANELGIPLYEYLGGKFGYRLPVPLMNVINGGAHADNNL
DIQEFMIVPVCGGAFREALRAGVETFHHLKKILKEKGYSTNVGDEGGFAPNLNSSEEALDILMQAIEKAGYKPGEDILLA
LDVASSEFYENGVYKFEGKERSAEEMIEFYEKLIQKYPIISIEDPMSENDWEGWKEITKRLGDKVQLVGDDLFTTNPKIL
RKGIEEGVANAILVKLNQIGTVSETLDTVMLAKERNYSAIISHRSGETEDTFISHLAVATNAGQIKTGSASRTDRIAKYN
ELLRIEERLGNGAVFWGREEFYRFTS
>Mature_425_residues
SRIKRVHGREVLDSRGNPTVEVEVELESGALGRAIVPSGASTGEREALELRDGDPKRYLGKGVLKAVDNVNGVIAKALVG
LEPYNQREIDQILIELDGTENKSKLGANAILGTSMAVARAAANELGIPLYEYLGGKFGYRLPVPLMNVINGGAHADNNLD
IQEFMIVPVCGGAFREALRAGVETFHHLKKILKEKGYSTNVGDEGGFAPNLNSSEEALDILMQAIEKAGYKPGEDILLAL
DVASSEFYENGVYKFEGKERSAEEMIEFYEKLIQKYPIISIEDPMSENDWEGWKEITKRLGDKVQLVGDDLFTTNPKILR
KGIEEGVANAILVKLNQIGTVSETLDTVMLAKERNYSAIISHRSGETEDTFISHLAVATNAGQIKTGSASRTDRIAKYNE
LLRIEERLGNGAVFWGREEFYRFTS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=51.7482517482517, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.1627906976744, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.1627906976744, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=51.981351981352, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.0280373831776, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=352, Percent_Identity=25.8522727272727, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI169201757, Length=352, Percent_Identity=25.8522727272727, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI239744207, Length=352, Percent_Identity=25.8522727272727, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=60.5140186915888, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=50.3480278422274, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=50.3480278422274, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=43.6842105263158, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=438, Percent_Identity=50, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=438, Percent_Identity=50, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=438, Percent_Identity=50, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=49.8855835240275, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=49.4279176201373, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.7674418604651, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.7674418604651, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.7674418604651, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.7674418604651, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=49.7674418604651, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=49.7674418604651, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_AQUAE (O66778)

Other databases:

- EMBL:   AE000657
- PIR:   F70343
- RefSeq:   NP_213338.1
- ProteinModelPortal:   O66778
- SMR:   O66778
- GeneID:   1193196
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_484
- NMPDR:   fig|224324.1.peg.353
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   O66778
- ProtClustDB:   PRK00077
- BioCyc:   AAEO224324:AQ_484-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46871; Mature: 46740

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 335-335 BINDING 155-155 BINDING 164-164 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKRVHGREVLDSRGNPTVEVEVELESGALGRAIVPSGASTGEREALELRDGDPKRYL
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVDNVNGVIAKALVGLEPYNQREIDQILIELDGTENKSKLGANAILGTSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAANELGIPLYEYLGGKFGYRLPVPLMNVINGGAHADNNLDIQEFMIVPVCGGAFREALR
HHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
AGVETFHHLKKILKEKGYSTNVGDEGGFAPNLNSSEEALDILMQAIEKAGYKPGEDILLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSEFYENGVYKFEGKERSAEEMIEFYEKLIQKYPIISIEDPMSENDWEGWKEITKR
EECHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHH
LGDKVQLVGDDLFTTNPKILRKGIEEGVANAILVKLNQIGTVSETLDTVMLAKERNYSAI
CCCEEEEECCCHHCCCHHHHHHHHHHHHHHEEHHHHHHCCCHHHHHHHHHHHHCCCCEEE
ISHRSGETEDTFISHLAVATNAGQIKTGSASRTDRIAKYNELLRIEERLGNGAVFWGREE
EECCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHH
FYRFTS
HHHCCC
>Mature Secondary Structure 
SRIKRVHGREVLDSRGNPTVEVEVELESGALGRAIVPSGASTGEREALELRDGDPKRYL
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVDNVNGVIAKALVGLEPYNQREIDQILIELDGTENKSKLGANAILGTSMAVAR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAANELGIPLYEYLGGKFGYRLPVPLMNVINGGAHADNNLDIQEFMIVPVCGGAFREALR
HHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
AGVETFHHLKKILKEKGYSTNVGDEGGFAPNLNSSEEALDILMQAIEKAGYKPGEDILLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSEFYENGVYKFEGKERSAEEMIEFYEKLIQKYPIISIEDPMSENDWEGWKEITKR
EECHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHH
LGDKVQLVGDDLFTTNPKILRKGIEEGVANAILVKLNQIGTVSETLDTVMLAKERNYSAI
CCCEEEEECCCHHCCCHHHHHHHHHHHHHHEEHHHHHHCCCHHHHHHHHHHHHCCCCEEE
ISHRSGETEDTFISHLAVATNAGQIKTGSASRTDRIAKYNELLRIEERLGNGAVFWGREE
EECCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHH
FYRFTS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320