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The map label for this gene is pcnB1 [H]

Identifier: 15605911

GI number: 15605911

Start: 279846

End: 282320

Strand: Direct

Name: pcnB1 [H]

Synonym: aq_411

Alternate gene names: 15605911

Gene position: 279846-282320 (Clockwise)

Preceding gene: 15605905

Following gene: 15605912

Centisome position: 18.04

GC content: 44.2

Gene sequence:

>2475_bases
ATGGTCTGTCCAAAAGTTGTTATTCTTTCCGAAGGTGCGGATCTCGACAGCCTTTCCGCAGCTTACGGAGTCCTGAAACT
TTACCCTGACGCATACTTGCTAAAGCCCAAGCACTTGTCCAAAAAAGCGGGGGAAGTTTTTAAGAAGTACAGGGATAAGT
TCAGAGTAATAGAAGACCTGCCAGACTGCTTTGAACTCGTGCTCGTGGATACACACTTTCTTCCGGAAGGTTTACCAAGA
GAAAGGATTAAAAGAATTATCGTTTACGACCACCATCCAATAGGGGACGTGAAGGAATTTGAGGGGAAAATTGAAAAGGT
TGGGGCGGCGACCACGCTCGTGGTGGAGGAAATTAAGGAGAAAGGTATAGATATAAATCCACGAGACGCTACACTTCTCG
CCTTCGGGATATACGAAGATACGGGAAATTTTACCTACGAAGGGACTACACCAAGGGACGCTTTGGCTTTAGCATTCTTG
CTTGAAAAGGGAGCAAACCTCAGGGAAATAAGGGAAGTCGTTATGGAAACCTATACGCCCGAGCAGATAGAGGCGGTAGG
GAAAATCGTCCAGTCTATAGAAAAGGTGTTCATAAACGGTAGGCAAATAAGTTTTGCAACTGCCGTTCTGGAGAGGTATC
AGCCGGATATAAACACGCTCCTTTACGAAATAAAAGACCTTAAAGAGTCTGATGCCTTCTTTGTGATAATAGAAGCGGAA
GGAAAGACTTACGTCTTCGGTCGTTCCCAGAGCGAAGATGTAGATGTAGGGGAAATACTTTCACACTTCGGCGGTGGTGG
GCACAGGGAGGCTGGAGCGGTAAAGCTCGAAAACGTATCAGCAGAACGGATAAAAGAGTTAATAAAAGCCTTTTTAAAGA
GAAAGTATGTCAAACTTAAGGTAAGAGACATTATGAACACACCGCCCTTCGTTTTAGAAGAACACGTAAGCGTAAAAGAC
GCACTCACAGAACTCAGTGAAAGGGGGATAGCGAACGCACCCGTGATAAACAGGGAGGGAAAACTGGTAGGGATAATCTC
AAAGAAGGCGTTGCTCAAACTTGTGAAACTCTATCCCGATGAACCCATAGAGCTCTTCGTCAACAGAGATTTTTACACCC
TTTCACCTGACGCACCTGTATGGGAAGCGGAGGAAATCCTCACAAAGTTTGGACAGAAATTAATCCCCGTAGTGGAAGAC
GGAACGGTTGTAGGAGTAGTTACGAGGCTAGACATACTCCAGGCGGTAAAGGAGGATTTAGAAAAGTTAAAGGAAAAGAG
GAGAAAGATAAAAGTTCCCGAAAACATAGAAGAAATAGCGAGGGAAGTGGGACAGATAGCAAAGGAAATGGGCCTCAGGG
CTTACATAGTGGGAGGAGTTGTGAGGGATATTTTACTCGGTAAAGAGGTTTGGGACGTTGACTTCGTAGTTGAGGGGAAT
GCCATAGAACTTGCAAAGGAACTCGCGAGGAGGCACGGCGTTAACGTCCACCCATTCCCCGAATTCGGCACAGCTCACCT
GAAAATCGGAAAACTGAAACTTGAATTTGCAACAGCACGGAGGGAAACTTACCCAAGACCCGGAGCCTATCCGAAAGTGG
AACCAGCCAGCTTGAAAGAGGACTTAATAAGGAGGGACTTCACGATAAATGCCATGGCAATATCCGTAAATCTCGAGGAC
TACGGAACACTCATAGATTACTTCGGAGGATTAAGAGATCTAAAAGACAAAGTAATAAGGGTTCTTCACCCTGTAAGTTT
TATAGAGGACCCTGTAAGGATTTTACGTGCCCTAAGATTTGCGGGAAGACTCAATTTTAAACTCTCAAGAAGCACGGAAA
AATTGTTAAAACAGGCTGTGAACCTCGGACTCCTAAAGGAAGCACCCAGGGGAAGGCTCATAAACGAGATAAAACTCGCC
CTCAGGGAAGACAGGTTTTTAGAAATTTTAGAACTCTACAGGAAGTACAGAGTATTAGAGGAGATAATTGAAGGTTTCCA
GTGGAACGAAAAGGTTCTCCAGAAACTCTACGCCCTCAGGAAAGTGGTGGACTGGCACGCTTTGGAGTTCTCCGAAGAGA
GAATAGATTACGGCTGGCTATACCTCCTTATACTCATATCTAATTTAGATTACGAAAGGGGAAAACACTTTCTTGAGGAG
ATGTCCGCTCCCTCCTGGGTTCGGGAGACCTACAAGTTTATGAAGTTCAAACTTGGTTCACTTAAAGAAGAACTCAAAAA
AGCGAAGGAGAATTACGAAGTTTACAGACTCCTCAAACCTCTTCACACTTCCGTTCTATTACTCCTAATGCTCGAGGAGG
AATTAAAAGAAAAGATAAAACTCTACCTCGAAAAACTCAGGAAGGTAAAACTTCCCAAGGAAAAGATTGAGGAACTGAAA
AAGCAAGGTCTTAAAGGAAAGGAACTCGGAGAGAGGATTGAAGAACTGAAAAGGGAAATTATGAATAAAATTTAG

Upstream 100 bases:

>100_bases
AAGCATCACTCACTAAGACCTATCCACCCTTATTCAATTGCCAAGGAGCCCGAAAGCCCTCAGCAGGGCCTTATTTAAAC
ATTATAATCATAAAACATAT

Downstream 100 bases:

>100_bases
TTGTGCTTGAAGTCAGGGAAGTAGAAAAGAGCTTTAAGAAGAAAAAGGTTTTAAAGGGGGGTTCCCTTTCCGTTAATAAA
GGTGAAATAGTTGGTCTTCT

Product: poly A polymerase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 824; Mature: 824

Protein sequence:

>824_residues
MVCPKVVILSEGADLDSLSAAYGVLKLYPDAYLLKPKHLSKKAGEVFKKYRDKFRVIEDLPDCFELVLVDTHFLPEGLPR
ERIKRIIVYDHHPIGDVKEFEGKIEKVGAATTLVVEEIKEKGIDINPRDATLLAFGIYEDTGNFTYEGTTPRDALALAFL
LEKGANLREIREVVMETYTPEQIEAVGKIVQSIEKVFINGRQISFATAVLERYQPDINTLLYEIKDLKESDAFFVIIEAE
GKTYVFGRSQSEDVDVGEILSHFGGGGHREAGAVKLENVSAERIKELIKAFLKRKYVKLKVRDIMNTPPFVLEEHVSVKD
ALTELSERGIANAPVINREGKLVGIISKKALLKLVKLYPDEPIELFVNRDFYTLSPDAPVWEAEEILTKFGQKLIPVVED
GTVVGVVTRLDILQAVKEDLEKLKEKRRKIKVPENIEEIAREVGQIAKEMGLRAYIVGGVVRDILLGKEVWDVDFVVEGN
AIELAKELARRHGVNVHPFPEFGTAHLKIGKLKLEFATARRETYPRPGAYPKVEPASLKEDLIRRDFTINAMAISVNLED
YGTLIDYFGGLRDLKDKVIRVLHPVSFIEDPVRILRALRFAGRLNFKLSRSTEKLLKQAVNLGLLKEAPRGRLINEIKLA
LREDRFLEILELYRKYRVLEEIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGWLYLLILISNLDYERGKHFLEE
MSAPSWVRETYKFMKFKLGSLKEELKKAKENYEVYRLLKPLHTSVLLLLMLEEELKEKIKLYLEKLRKVKLPKEKIEELK
KQGLKGKELGERIEELKREIMNKI

Sequences:

>Translated_824_residues
MVCPKVVILSEGADLDSLSAAYGVLKLYPDAYLLKPKHLSKKAGEVFKKYRDKFRVIEDLPDCFELVLVDTHFLPEGLPR
ERIKRIIVYDHHPIGDVKEFEGKIEKVGAATTLVVEEIKEKGIDINPRDATLLAFGIYEDTGNFTYEGTTPRDALALAFL
LEKGANLREIREVVMETYTPEQIEAVGKIVQSIEKVFINGRQISFATAVLERYQPDINTLLYEIKDLKESDAFFVIIEAE
GKTYVFGRSQSEDVDVGEILSHFGGGGHREAGAVKLENVSAERIKELIKAFLKRKYVKLKVRDIMNTPPFVLEEHVSVKD
ALTELSERGIANAPVINREGKLVGIISKKALLKLVKLYPDEPIELFVNRDFYTLSPDAPVWEAEEILTKFGQKLIPVVED
GTVVGVVTRLDILQAVKEDLEKLKEKRRKIKVPENIEEIAREVGQIAKEMGLRAYIVGGVVRDILLGKEVWDVDFVVEGN
AIELAKELARRHGVNVHPFPEFGTAHLKIGKLKLEFATARRETYPRPGAYPKVEPASLKEDLIRRDFTINAMAISVNLED
YGTLIDYFGGLRDLKDKVIRVLHPVSFIEDPVRILRALRFAGRLNFKLSRSTEKLLKQAVNLGLLKEAPRGRLINEIKLA
LREDRFLEILELYRKYRVLEEIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGWLYLLILISNLDYERGKHFLEE
MSAPSWVRETYKFMKFKLGSLKEELKKAKENYEVYRLLKPLHTSVLLLLMLEEELKEKIKLYLEKLRKVKLPKEKIEELK
KQGLKGKELGERIEELKREIMNKI
>Mature_824_residues
MVCPKVVILSEGADLDSLSAAYGVLKLYPDAYLLKPKHLSKKAGEVFKKYRDKFRVIEDLPDCFELVLVDTHFLPEGLPR
ERIKRIIVYDHHPIGDVKEFEGKIEKVGAATTLVVEEIKEKGIDINPRDATLLAFGIYEDTGNFTYEGTTPRDALALAFL
LEKGANLREIREVVMETYTPEQIEAVGKIVQSIEKVFINGRQISFATAVLERYQPDINTLLYEIKDLKESDAFFVIIEAE
GKTYVFGRSQSEDVDVGEILSHFGGGGHREAGAVKLENVSAERIKELIKAFLKRKYVKLKVRDIMNTPPFVLEEHVSVKD
ALTELSERGIANAPVINREGKLVGIISKKALLKLVKLYPDEPIELFVNRDFYTLSPDAPVWEAEEILTKFGQKLIPVVED
GTVVGVVTRLDILQAVKEDLEKLKEKRRKIKVPENIEEIAREVGQIAKEMGLRAYIVGGVVRDILLGKEVWDVDFVVEGN
AIELAKELARRHGVNVHPFPEFGTAHLKIGKLKLEFATARRETYPRPGAYPKVEPASLKEDLIRRDFTINAMAISVNLED
YGTLIDYFGGLRDLKDKVIRVLHPVSFIEDPVRILRALRFAGRLNFKLSRSTEKLLKQAVNLGLLKEAPRGRLINEIKLA
LREDRFLEILELYRKYRVLEEIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGWLYLLILISNLDYERGKHFLEE
MSAPSWVRETYKFMKFKLGSLKEELKKAKENYEVYRLLKPLHTSVLLLLMLEEELKEKIKLYLEKLRKVKLPKEKIEELK
KQGLKGKELGERIEELKREIMNKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=154, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1789436, Length=226, Percent_Identity=33.6283185840708, Blast_Score=93, Evalue=9e-20,
Organism=Escherichia coli, GI87081691, Length=244, Percent_Identity=33.1967213114754, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=176, Percent_Identity=33.5227272727273, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 94659; Mature: 94659

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCPKVVILSEGADLDSLSAAYGVLKLYPDAYLLKPKHLSKKAGEVFKKYRDKFRVIEDL
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
PDCFELVLVDTHFLPEGLPRERIKRIIVYDHHPIGDVKEFEGKIEKVGAATTLVVEEIKE
HHHHHHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGIDINPRDATLLAFGIYEDTGNFTYEGTTPRDALALAFLLEKGANLREIREVVMETYTP
CCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EQIEAVGKIVQSIEKVFINGRQISFATAVLERYQPDINTLLYEIKDLKESDAFFVIIEAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEC
GKTYVFGRSQSEDVDVGEILSHFGGGGHREAGAVKLENVSAERIKELIKAFLKRKYVKLK
CCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
VRDIMNTPPFVLEEHVSVKDALTELSERGIANAPVINREGKLVGIISKKALLKLVKLYPD
HHHHCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCEECCCCCEEEEHHHHHHHHHHHHCCC
EPIELFVNRDFYTLSPDAPVWEAEEILTKFGQKLIPVVEDGTVVGVVTRLDILQAVKEDL
CCEEEEECCCEEEECCCCCCCCHHHHHHHHCHHHCCEECCCCEEHHHHHHHHHHHHHHHH
EKLKEKRRKIKVPENIEEIAREVGQIAKEMGLRAYIVGGVVRDILLGKEVWDVDFVVEGN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECC
AIELAKELARRHGVNVHPFPEFGTAHLKIGKLKLEFATARRETYPRPGAYPKVEPASLKE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHH
DLIRRDFTINAMAISVNLEDYGTLIDYFGGLRDLKDKVIRVLHPVSFIEDPVRILRALRF
HHHHCCCEEEEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLNFKLSRSTEKLLKQAVNLGLLKEAPRGRLINEIKLALREDRFLEILELYRKYRVLE
HHCCCEEECHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGWLYLLILISNLDYERGKHFLEE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHH
MSAPSWVRETYKFMKFKLGSLKEELKKAKENYEVYRLLKPLHTSVLLLLMLEEELKEKIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLEKLRKVKLPKEKIEELKKQGLKGKELGERIEELKREIMNKI
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVCPKVVILSEGADLDSLSAAYGVLKLYPDAYLLKPKHLSKKAGEVFKKYRDKFRVIEDL
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
PDCFELVLVDTHFLPEGLPRERIKRIIVYDHHPIGDVKEFEGKIEKVGAATTLVVEEIKE
HHHHHHHHHHHHHCCCCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KGIDINPRDATLLAFGIYEDTGNFTYEGTTPRDALALAFLLEKGANLREIREVVMETYTP
CCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCH
EQIEAVGKIVQSIEKVFINGRQISFATAVLERYQPDINTLLYEIKDLKESDAFFVIIEAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEC
GKTYVFGRSQSEDVDVGEILSHFGGGGHREAGAVKLENVSAERIKELIKAFLKRKYVKLK
CCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
VRDIMNTPPFVLEEHVSVKDALTELSERGIANAPVINREGKLVGIISKKALLKLVKLYPD
HHHHCCCCCHHHHHHCHHHHHHHHHHHCCCCCCCEECCCCCEEEEHHHHHHHHHHHHCCC
EPIELFVNRDFYTLSPDAPVWEAEEILTKFGQKLIPVVEDGTVVGVVTRLDILQAVKEDL
CCEEEEECCCEEEECCCCCCCCHHHHHHHHCHHHCCEECCCCEEHHHHHHHHHHHHHHHH
EKLKEKRRKIKVPENIEEIAREVGQIAKEMGLRAYIVGGVVRDILLGKEVWDVDFVVEGN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECC
AIELAKELARRHGVNVHPFPEFGTAHLKIGKLKLEFATARRETYPRPGAYPKVEPASLKE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHH
DLIRRDFTINAMAISVNLEDYGTLIDYFGGLRDLKDKVIRVLHPVSFIEDPVRILRALRF
HHHHCCCEEEEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLNFKLSRSTEKLLKQAVNLGLLKEAPRGRLINEIKLALREDRFLEILELYRKYRVLE
HHCCCEEECHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGWLYLLILISNLDYERGKHFLEE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHH
MSAPSWVRETYKFMKFKLGSLKEELKKAKENYEVYRLLKPLHTSVLLLLMLEEELKEKIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLEKLRKVKLPKEKIEELKKQGLKGKELGERIEELKREIMNKI
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA