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The map label for this gene is gcvH5

Identifier: 15605903

GI number: 15605903

Start: 272598

End: 273044

Strand: Direct

Name: gcvH5

Synonym: aq_402

Alternate gene names: 15605903

Gene position: 272598-273044 (Clockwise)

Preceding gene: 15605902

Following gene: 15605904

Centisome position: 17.57

GC content: 50.34

Gene sequence:

>447_bases
ATGGCAGAAGTAAACGGGTGCCAACTGCCCGAGGATAGACTCTACTACATCAACCCAGATAAGAACGCCTTCATTTGGGC
AAAGGAAGAGGACGGGGTGTTTACCATCGGGCTCACTTCAGTTGCAGCCGCTATGGCGGGAAGACTCGTCGCTTACACGC
CCAAGAAGGTAGGAAAGGCTGTTAAAAAGGACAAGAGCGTGGCAACCATTGAAAGCGGAAAGTGGGTTGGACCCGTACCT
TCTCCCTTTGACGGGGAAATCGTTGAAGTTAACGAAGCTCTAAAGGGAAATCCCGCTCTCGTGAACGACGATCCCTACGG
ACAGGGCTGGATAGCTAAGGTAAAACCTGCAAATCCCGAAGAAGCTAAGTCCGTTCTCGTTGACGCGAACAAAGCGGCTG
AACTTCTCCAACCAATAGTTCAAGAAAAAGGAGTTAAGTGCGGTTAA

Upstream 100 bases:

>100_bases
GCTTGACGGTATAGTGCCCGCAAGCGAGTTTGCACTGAGGGCTATGATGGCGGATAAGCTTATAACCTTCTAGTTTCAAT
AAATTTAAGGGAGGTGTGCT

Downstream 100 bases:

>100_bases
TTCCCCTTCTCTCTCCTTTTAAAACCTTTTGAGGGAGCGGTAATGAATGTATTCGGTGTAATACAGGAGCTTGACGTAGA
ACTTAATGAAAAATTAGTGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAEVNGCQLPEDRLYYINPDKNAFIWAKEEDGVFTIGLTSVAAAMAGRLVAYTPKKVGKAVKKDKSVATIESGKWVGPVP
SPFDGEIVEVNEALKGNPALVNDDPYGQGWIAKVKPANPEEAKSVLVDANKAAELLQPIVQEKGVKCG

Sequences:

>Translated_148_residues
MAEVNGCQLPEDRLYYINPDKNAFIWAKEEDGVFTIGLTSVAAAMAGRLVAYTPKKVGKAVKKDKSVATIESGKWVGPVP
SPFDGEIVEVNEALKGNPALVNDDPYGQGWIAKVKPANPEEAKSVLVDANKAAELLQPIVQEKGVKCG
>Mature_147_residues
AEVNGCQLPEDRLYYINPDKNAFIWAKEEDGVFTIGLTSVAAAMAGRLVAYTPKKVGKAVKKDKSVATIESGKWVGPVPS
PFDGEIVEVNEALKGNPALVNDDPYGQGWIAKVKPANPEEAKSVLVDANKAAELLQPIVQEKGVKCG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=103, Percent_Identity=33.9805825242718, Blast_Score=71, Evalue=4e-13,
Organism=Homo sapiens, GI89057342, Length=103, Percent_Identity=33.0097087378641, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789271, Length=110, Percent_Identity=40, Blast_Score=76, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH5_AQUAE (O66720)

Other databases:

- EMBL:   AE000657
- PIR:   D70336
- RefSeq:   NP_213280.1
- ProteinModelPortal:   O66720
- SMR:   O66720
- GeneID:   1193046
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_402
- NMPDR:   fig|224324.1.peg.295
- HOGENOM:   HBG693789
- OMA:   GKWVGPV
- ProtClustDB:   PRK13380
- BioCyc:   AAEO224324:AQ_402-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 15823; Mature: 15692

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVNGCQLPEDRLYYINPDKNAFIWAKEEDGVFTIGLTSVAAAMAGRLVAYTPKKVGKA
CCCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEHHHHHHHHHCCEEEECCHHHHHH
VKKDKSVATIESGKWVGPVPSPFDGEIVEVNEALKGNPALVNDDPYGQGWIAKVKPANPE
HHCCCCEEEECCCCEECCCCCCCCCCEEEECHHHCCCCCEECCCCCCCCEEEEECCCCHH
EAKSVLVDANKAAELLQPIVQEKGVKCG
HHHHEEECCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEVNGCQLPEDRLYYINPDKNAFIWAKEEDGVFTIGLTSVAAAMAGRLVAYTPKKVGKA
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCEEEEEHHHHHHHHHCCEEEECCHHHHHH
VKKDKSVATIESGKWVGPVPSPFDGEIVEVNEALKGNPALVNDDPYGQGWIAKVKPANPE
HHCCCCEEEECCCCEECCCCCCCCCCEEEECHHHCCCCCEECCCCCCCCEEEEECCCCHH
EAKSVLVDANKAAELLQPIVQEKGVKCG
HHHHEEECCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320