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The map label for this gene is ribA

Identifier: 15605862

GI number: 15605862

Start: 228716

End: 229936

Strand: Direct

Name: ribA

Synonym: aq_350

Alternate gene names: 15605862

Gene position: 228716-229936 (Clockwise)

Preceding gene: 15605859

Following gene: 15605866

Centisome position: 14.74

GC content: 48.32

Gene sequence:

>1221_bases
ATGAGTGAGAGGGAAGAGTTCAAGTTCAACACGGTGGAAGAAGCAATAGAAGACATAAGACAGGGGAAGATGGTAATAGT
TGTGGACGACCCAGACAGGGAAAACGAAGGAGACCTCGTAATGGCTGCAGAAAAGGTTACCCCTGAGGCCATAAACTTCA
TGGCAAAGTACGGGAGGGGACTAATATGCCTGTCACTCACTCCAGAAAGGTGTGAACAACTGGACCTCCACCCCATGACT
CCTATGAATACGGACCCAAAAGGTACTTACTTTTGCGTTTCCATAGATGCCCACCCAAAGCATGGAACGACAACTGGAAT
TTCCGCCTACGACAGGGCCTTGACTATAAAACTCGCTATTTCCCCCGACGCTAAGCCCTCGGACTTCGTAAGACCAGGAC
ACGTTTTTCCCTTAAAGGCAAGACCTGGAGGAGTTTTGGAAAGGGCGGGACACACGGAGGCTTCCGTTGACCTTGCAAGA
CTCGCTGGACTCTACCCCGCCGGAGTTATTTGTGAGATTATGAAAGACGACGGAACCATGGCGAGGGTTCCCGACCTTAT
GGAGTTTGCGAAAAAGCACAACCTGAAAATAATCACAATAGCGGATCTAATAAAGTACAGGTTGAGGAGAGAAACCTTAG
TAGAGAAGGTTGCAAGTGCTCACCTTCCCACTCCCTGGGGAGTTTTCAAGATACACGCTTACAGGCACAAACTCACTGGT
GAAGAGCAGGTGGCCCTCACGATGGGTGAGTGGAAAGAAGACGAACCCGTTCTCGTCAGAGTTCACTCGGAATGCCTGAC
TGGAGACGTTTTCCGTTCCTTCAGGTGTGACTGCAGACCTCAGCTTGAGAAAGCCCTTGAGATGATAGCAAAGGAAGGAA
AAGGAGTTCTCGTTTACATACTCGGGCACGAGGGAAGGGGTATAGGAATAGCGAACAAAATTAAAGCTTACGAACTTCAG
GAGAAGGGATACGACACGGTGGAGGCGAACGAAAAACTCGGATACCCACCTGACCTCAGGGACTACGGTATAGGGGCACA
GATACTCAGAGACCTCGGGGTCAGAAAAATGAAACTAATGACGAATAACCCGAGGAAGATTGTGGCTCTTGAAGGCTTCG
GACTGGAAGTAGTTGAAAGAGTACCTATAAAAATAGAACCAAATCCTTATAACAAAATTTACCTGCAGGTTAAAAAGGAC
AAGCTGGGGCACATGTTCTAA

Upstream 100 bases:

>100_bases
GTTTCCTTGGTAAAAGCTTAACCTTTACTCTCCTCATTTTCCATTCCTCCGTAGATCAGAAAACTTTATTATACATAAGT
TTTTCTTTAAATTTATTCCT

Downstream 100 bases:

>100_bases
GCTCTTCGTGCCCCGAGTTCTTCCAATCTCTTCTTTAGTTCTTCAACCTCGTCCTCTTCGGAAAGTCCCAAGTAATCTAA
GAGCTTTTTAACACTTACCC

Product: GTP cyclohydrolase II

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSEREEFKFNTVEEAIEDIRQGKMVIVVDDPDRENEGDLVMAAEKVTPEAINFMAKYGRGLICLSLTPERCEQLDLHPMT
PMNTDPKGTYFCVSIDAHPKHGTTTGISAYDRALTIKLAISPDAKPSDFVRPGHVFPLKARPGGVLERAGHTEASVDLAR
LAGLYPAGVICEIMKDDGTMARVPDLMEFAKKHNLKIITIADLIKYRLRRETLVEKVASAHLPTPWGVFKIHAYRHKLTG
EEQVALTMGEWKEDEPVLVRVHSECLTGDVFRSFRCDCRPQLEKALEMIAKEGKGVLVYILGHEGRGIGIANKIKAYELQ
EKGYDTVEANEKLGYPPDLRDYGIGAQILRDLGVRKMKLMTNNPRKIVALEGFGLEVVERVPIKIEPNPYNKIYLQVKKD
KLGHMF

Sequences:

>Translated_406_residues
MSEREEFKFNTVEEAIEDIRQGKMVIVVDDPDRENEGDLVMAAEKVTPEAINFMAKYGRGLICLSLTPERCEQLDLHPMT
PMNTDPKGTYFCVSIDAHPKHGTTTGISAYDRALTIKLAISPDAKPSDFVRPGHVFPLKARPGGVLERAGHTEASVDLAR
LAGLYPAGVICEIMKDDGTMARVPDLMEFAKKHNLKIITIADLIKYRLRRETLVEKVASAHLPTPWGVFKIHAYRHKLTG
EEQVALTMGEWKEDEPVLVRVHSECLTGDVFRSFRCDCRPQLEKALEMIAKEGKGVLVYILGHEGRGIGIANKIKAYELQ
EKGYDTVEANEKLGYPPDLRDYGIGAQILRDLGVRKMKLMTNNPRKIVALEGFGLEVVERVPIKIEPNPYNKIYLQVKKD
KLGHMF
>Mature_405_residues
SEREEFKFNTVEEAIEDIRQGKMVIVVDDPDRENEGDLVMAAEKVTPEAINFMAKYGRGLICLSLTPERCEQLDLHPMTP
MNTDPKGTYFCVSIDAHPKHGTTTGISAYDRALTIKLAISPDAKPSDFVRPGHVFPLKARPGGVLERAGHTEASVDLARL
AGLYPAGVICEIMKDDGTMARVPDLMEFAKKHNLKIITIADLIKYRLRRETLVEKVASAHLPTPWGVFKIHAYRHKLTGE
EQVALTMGEWKEDEPVLVRVHSECLTGDVFRSFRCDCRPQLEKALEMIAKEGKGVLVYILGHEGRGIGIANKIKAYELQE
KGYDTVEANEKLGYPPDLRDYGIGAQILRDLGVRKMKLMTNNPRKIVALEGFGLEVVERVPIKIEPNPYNKIYLQVKKDK
LGHMF

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family

Homologues:

Organism=Escherichia coli, GI1789420, Length=203, Percent_Identity=52.7093596059113, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=50.259067357513, Blast_Score=201, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=43.9613526570048, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319438, Length=186, Percent_Identity=40.8602150537634, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBBA_AQUAE (O66679)

Other databases:

- EMBL:   AE000657
- PIR:   C70331
- RefSeq:   NP_213239.1
- ProteinModelPortal:   O66679
- GeneID:   1193005
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_350
- NMPDR:   fig|224324.1.peg.254
- HOGENOM:   HBG735778
- OMA:   RCDCRMQ
- PhylomeDB:   O66679
- ProtClustDB:   CLSK2299371
- BioCyc:   AAEO224324:AQ_350-MONOMER
- BRENDA:   3.5.4.25
- BRENDA:   4.1.99.12
- HAMAP:   MF_01283
- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299
- Gene3D:   G3DSA:3.90.870.10
- PIRSF:   PIRSF001259
- TIGRFAMs:   TIGR00505
- TIGRFAMs:   TIGR00506

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: =4.1.99.12; =3.5.4.25

Molecular weight: Translated: 45578; Mature: 45446

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 340-340 BINDING 38-38 BINDING 172-172 BINDING 281-281 BINDING 326-326 BINDING 361-361 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEREEFKFNTVEEAIEDIRQGKMVIVVDDPDRENEGDLVMAAEKVTPEAINFMAKYGRG
CCCCHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCC
LICLSLTPERCEQLDLHPMTPMNTDPKGTYFCVSIDAHPKHGTTTGISAYDRALTIKLAI
EEEEEECHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
SPDAKPSDFVRPGHVFPLKARPGGVLERAGHTEASVDLARLAGLYPAGVICEIMKDDGTM
CCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCE
ARVPDLMEFAKKHNLKIITIADLIKYRLRRETLVEKVASAHLPTPWGVFKIHAYRHKLTG
ECCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCC
EEQVALTMGEWKEDEPVLVRVHSECLTGDVFRSFRCDCRPQLEKALEMIAKEGKGVLVYI
CCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEE
LGHEGRGIGIANKIKAYELQEKGYDTVEANEKLGYPPDLRDYGIGAQILRDLGVRKMKLM
ECCCCCCCCCHHCCEEEEHHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHCCCEEEEEE
TNNPRKIVALEGFGLEVVERVPIKIEPNPYNKIYLQVKKDKLGHMF
ECCCCEEEEEECCCHHHHHCCCEEECCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
SEREEFKFNTVEEAIEDIRQGKMVIVVDDPDRENEGDLVMAAEKVTPEAINFMAKYGRG
CCCHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCC
LICLSLTPERCEQLDLHPMTPMNTDPKGTYFCVSIDAHPKHGTTTGISAYDRALTIKLAI
EEEEEECHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEEE
SPDAKPSDFVRPGHVFPLKARPGGVLERAGHTEASVDLARLAGLYPAGVICEIMKDDGTM
CCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCE
ARVPDLMEFAKKHNLKIITIADLIKYRLRRETLVEKVASAHLPTPWGVFKIHAYRHKLTG
ECCHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHCCC
EEQVALTMGEWKEDEPVLVRVHSECLTGDVFRSFRCDCRPQLEKALEMIAKEGKGVLVYI
CCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEE
LGHEGRGIGIANKIKAYELQEKGYDTVEANEKLGYPPDLRDYGIGAQILRDLGVRKMKLM
ECCCCCCCCCHHCCEEEEHHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHCCCEEEEEE
TNNPRKIVALEGFGLEVVERVPIKIEPNPYNKIYLQVKKDKLGHMF
ECCCCEEEEEECCCHHHHHCCCEEECCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320