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The map label for this gene is kdtA [H]

Identifier: 15605846

GI number: 15605846

Start: 212605

End: 213666

Strand: Direct

Name: kdtA [H]

Synonym: aq_326

Alternate gene names: 15605846

Gene position: 212605-213666 (Clockwise)

Preceding gene: 15605844

Following gene: 15605848

Centisome position: 13.7

GC content: 37.29

Gene sequence:

>1062_bases
ATGCAATTTGAAGTCCTGAAGAGGTTTTTCCCTAAAGAGAGTCTGAAAAATTGCAAAGGAGCTTTATGGGTTCACACAGC
AAGTATAGGAGAATTCAATACTTTCCTACCTATCCTTAAAGAACTAAAAAGAGAACACAGAATTCTGCTCACTTACTTTT
CCCCCCGTGCAAGGGAATACCTAAAGACAAAAAGTGATTTTTACGACTGCCTTCATCCTCTCCCTCTTGATAACCCATTT
TCCGTAAAGAGATTTGAAGAACTATCAAAACCAAAAGCCTTAATAGTAGTTGAAAGGGAATTCTGGCCCTCTTTAATAAT
TTTTACTAAAGTTCCCAAAATCCTCGTCAACGCCTACGCAAAAGGGAGTTTAATAGAGAAAATTCTCTCAAAAAAATTTG
ACTTAATAATAATGAGAACTCAAGAAGATGTGGAAAAATTTAAAACCTTTGGTGCAAAGAGGGTATTTTCCTGCGGGAAT
TTAAAATTCATTTGTCAAAAAGGAAAAGGGATAAAATTGAAAGGTGAATTCATAGTAGCGGGAAGTATCCACACGGGCGA
AGTTGAAATCATTCTAAAAGCCTTTAAAGAAATAAAAAAGACTTATTCAAGCTTAAAACTGATACTTGTCCCGAGGCATA
TAGAGAACGCAAAAATCTTTGAAAAAAAGGCGAGGGACTTTGGTTTTAAAACTTCCTTCTTTGAAAACCTCGAAGGTGAT
GTTATCCTAGTGGACAGGTTCGGGATACTTAAAGAACTATACCCGGTCGGAAAAATCGCAATAGTTGGCGGAACTTTTGT
AAATATAGGGGGGCATAACCTTCTGGAGCCCACCTGCTGGGGCATTCCCGTAATTTACGGACCTTACACGCATAAAGTAA
ATGATCTGAAGGAATTTTTAGAAAAAGAGGGAGCTGGATTTGAAGTAAAGAATGAAACAGAGCTCGTGACAAAACTCACG
GAGCTCCTATCCGTAAAGAAAGAGATTAAAGTGGAGGAAAAATCAAGGGAAATTAAGGGATGTTACTTGGAAAAACTTAG
GGAGTTTTTAAGAGGTCTTTAA

Upstream 100 bases:

>100_bases
TCGGATAAGCTTCCCTTATGGCTTTTAAAACCGTCATTCCCCCCGCACCACTGTCAAAAATCCCTATTTTCATAAAGAAG
ATTTTACAATTCATTCCTTG

Downstream 100 bases:

>100_bases
CGAAGGGAATTACGACCTCGTTGAACTTTTCGGGGTTTTCAAAAGGCGGAAGGTGTGCGGAGTTTTCAAGCTCGTAGTAC
TTTGCTCCCTTTATGCCCTC

Product: 3-deoxy-D-manno-2-octulosonic acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQFEVLKRFFPKESLKNCKGALWVHTASIGEFNTFLPILKELKREHRILLTYFSPRAREYLKTKSDFYDCLHPLPLDNPF
SVKRFEELSKPKALIVVEREFWPSLIIFTKVPKILVNAYAKGSLIEKILSKKFDLIIMRTQEDVEKFKTFGAKRVFSCGN
LKFICQKGKGIKLKGEFIVAGSIHTGEVEIILKAFKEIKKTYSSLKLILVPRHIENAKIFEKKARDFGFKTSFFENLEGD
VILVDRFGILKELYPVGKIAIVGGTFVNIGGHNLLEPTCWGIPVIYGPYTHKVNDLKEFLEKEGAGFEVKNETELVTKLT
ELLSVKKEIKVEEKSREIKGCYLEKLREFLRGL

Sequences:

>Translated_353_residues
MQFEVLKRFFPKESLKNCKGALWVHTASIGEFNTFLPILKELKREHRILLTYFSPRAREYLKTKSDFYDCLHPLPLDNPF
SVKRFEELSKPKALIVVEREFWPSLIIFTKVPKILVNAYAKGSLIEKILSKKFDLIIMRTQEDVEKFKTFGAKRVFSCGN
LKFICQKGKGIKLKGEFIVAGSIHTGEVEIILKAFKEIKKTYSSLKLILVPRHIENAKIFEKKARDFGFKTSFFENLEGD
VILVDRFGILKELYPVGKIAIVGGTFVNIGGHNLLEPTCWGIPVIYGPYTHKVNDLKEFLEKEGAGFEVKNETELVTKLT
ELLSVKKEIKVEEKSREIKGCYLEKLREFLRGL
>Mature_353_residues
MQFEVLKRFFPKESLKNCKGALWVHTASIGEFNTFLPILKELKREHRILLTYFSPRAREYLKTKSDFYDCLHPLPLDNPF
SVKRFEELSKPKALIVVEREFWPSLIIFTKVPKILVNAYAKGSLIEKILSKKFDLIIMRTQEDVEKFKTFGAKRVFSCGN
LKFICQKGKGIKLKGEFIVAGSIHTGEVEIILKAFKEIKKTYSSLKLILVPRHIENAKIFEKKARDFGFKTSFFENLEGD
VILVDRFGILKELYPVGKIAIVGGTFVNIGGHNLLEPTCWGIPVIYGPYTHKVNDLKEFLEKEGAGFEVKNETELVTKLT
ELLSVKKEIKVEEKSREIKGCYLEKLREFLRGL

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=343, Percent_Identity=30.0291545189504, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 40677; Mature: 40677

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFEVLKRFFPKESLKNCKGALWVHTASIGEFNTFLPILKELKREHRILLTYFSPRAREY
CCHHHHHHHCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECHHHHHH
LKTKSDFYDCLHPLPLDNPFSVKRFEELSKPKALIVVEREFWPSLIIFTKVPKILVNAYA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHC
KGSLIEKILSKKFDLIIMRTQEDVEKFKTFGAKRVFSCGNLKFICQKGKGIKLKGEFIVA
CHHHHHHHHHCCCCEEEEECHHHHHHHHHHCHHHHCCCCCEEEEEECCCCEEEECCEEEE
GSIHTGEVEIILKAFKEIKKTYSSLKLILVPRHIENAKIFEKKARDFGFKTSFFENLEGD
ECCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCC
VILVDRFGILKELYPVGKIAIVGGTFVNIGGHNLLEPTCWGIPVIYGPYTHKVNDLKEFL
EEEEECHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
EKEGAGFEVKNETELVTKLTELLSVKKEIKVEEKSREIKGCYLEKLREFLRGL
HHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQFEVLKRFFPKESLKNCKGALWVHTASIGEFNTFLPILKELKREHRILLTYFSPRAREY
CCHHHHHHHCCHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECHHHHHH
LKTKSDFYDCLHPLPLDNPFSVKRFEELSKPKALIVVEREFWPSLIIFTKVPKILVNAYA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHC
KGSLIEKILSKKFDLIIMRTQEDVEKFKTFGAKRVFSCGNLKFICQKGKGIKLKGEFIVA
CHHHHHHHHHCCCCEEEEECHHHHHHHHHHCHHHHCCCCCEEEEEECCCCEEEECCEEEE
GSIHTGEVEIILKAFKEIKKTYSSLKLILVPRHIENAKIFEKKARDFGFKTSFFENLEGD
ECCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHCCCCC
VILVDRFGILKELYPVGKIAIVGGTFVNIGGHNLLEPTCWGIPVIYGPYTHKVNDLKEFL
EEEEECHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
EKEGAGFEVKNETELVTKLTELLSVKKEIKVEEKSREIKGCYLEKLREFLRGL
HHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]