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The map label for this gene is proC

Identifier: 15605736

GI number: 15605736

Start: 101014

End: 101811

Strand: Direct

Name: proC

Synonym: aq_166

Alternate gene names: 15605736

Gene position: 101014-101811 (Clockwise)

Preceding gene: 15605734

Following gene: 15605737

Centisome position: 6.51

GC content: 44.24

Gene sequence:

>798_bases
GTGCATGCTATTATCTTAACTATGCGCGTAGGGATAGTGGGTTTTGGGAATATGGGACAGGCTTTTGCCTTGTGCTTTTC
AAAAAAACTCGGTAAAGAAAACATAATAGTCACGGACAAGGTTCAGGAAAAGAGAAACCTCGCAACGGAGATGGGAATAG
CTTTTGCAAGTGATGTAAAATTCTTGGCGGACAACTCAGACGTTGTTCTCGTAGCGGTAAAGCCCAAGGACTCGCAGGAG
GTTTTACAAAAACTAAAGGATTACAAAGGGATAATTCTCAGCATAATGGCGGGAGTGAGTATTGAGAAAATGGAAAAAAT
CTTAGGAAAGGATAAAAAGATAGTAAGGGTAATGCCAAACGTAAACGTGGCCGTGGGAAGCGGTGTTATGGCGATAACCG
ACAACGGGAACCTGAGCGAAGAAGAAAGAAGTAAAGTAGAGGAACTTCTGCTTTCCTGCGGAACTCTTTACCGTATAGAG
GAAAGATTATTTGACGCCTTTACCGCACTTGCAGGAAGCGGACCCGCTTTCGTTTTCTCCTTTATAGACGCTCTGGCACT
TGCGGGAGTCCATCAAGGTTTTTCTTACGAACAAGCCCTCAGGATAGCCCTTGACACGGTTATGGGTAGTGCAAAACTCT
TGAAAGAGTTTCAGGTAAATCCTAACGAGCTCATAGCTAAGGTCACCTCTCCCGGCGGAACGACTATTGAAGGGATAAAG
TACCTTGAGGAAAAGGGATTTAAAGGCACGGTTATGGAATGTATAAACAGGACTTCCCAGAAGGCAAAGAAGCTTTGA

Upstream 100 bases:

>100_bases
CGAACTCTTCGTCTTTTTCAATCCTCCTTACTCTAAAATGCTTTACTTCTCCTTCCCTCAAAATAAGGAGGTTTCCGCGC
CTTTCTTCTGAGTAAAAGAC

Downstream 100 bases:

>100_bases
ATTTAGAGATAAGGCTGGGAAATAAACTCGTAAGATTCAGGAAATTCACAGGAAAGGAAAAGATTTACCTTCCCATTCAA
AGACACACCGATAAAGTTAT

Product: pyrroline carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MHAIILTMRVGIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLADNSDVVLVAVKPKDSQE
VLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIE
ERLFDAFTALAGSGPAFVFSFIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK
YLEEKGFKGTVMECINRTSQKAKKL

Sequences:

>Translated_265_residues
MHAIILTMRVGIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLADNSDVVLVAVKPKDSQE
VLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIE
ERLFDAFTALAGSGPAFVFSFIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK
YLEEKGFKGTVMECINRTSQKAKKL
>Mature_265_residues
MHAIILTMRVGIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVKFLADNSDVVLVAVKPKDSQE
VLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIE
ERLFDAFTALAGSGPAFVFSFIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK
YLEEKGFKGTVMECINRTSQKAKKL

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family

Homologues:

Organism=Homo sapiens, GI198041662, Length=264, Percent_Identity=31.4393939393939, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI24797095, Length=272, Percent_Identity=31.6176470588235, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI24797097, Length=272, Percent_Identity=31.6176470588235, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI21361454, Length=271, Percent_Identity=31.3653136531365, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI1786585, Length=263, Percent_Identity=34.9809885931559, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569021, Length=263, Percent_Identity=30.7984790874525, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17540664, Length=274, Percent_Identity=30.2919708029197, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320861, Length=285, Percent_Identity=28.0701754385965, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21358587, Length=267, Percent_Identity=31.8352059925094, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24648116, Length=278, Percent_Identity=31.294964028777, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24647700, Length=168, Percent_Identity=32.1428571428571, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P5CR_AQUAE (O66553)

Other databases:

- EMBL:   AE000657
- PIR:   F70315
- RefSeq:   NP_213113.1
- ProteinModelPortal:   O66553
- SMR:   O66553
- GeneID:   1192696
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_166
- NMPDR:   fig|224324.1.peg.128
- HOGENOM:   HBG726602
- OMA:   RTFNEHQ
- PhylomeDB:   O66553
- ProtClustDB:   CLSK2748256
- BioCyc:   AAEO224324:AQ_166-MONOMER
- BRENDA:   1.5.1.2
- GO:   GO:0005737
- GO:   GO:0005488
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11645
- PIRSF:   PIRSF000193
- TIGRFAMs:   TIGR00112

Pfam domain/function: PF03807 F420_oxidored; SSF48179 6DGDH_C_like

EC number: =1.5.1.2

Molecular weight: Translated: 28814; Mature: 28814

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAIILTMRVGIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVK
CCEEEEEEEHHHEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
FLADNSDVVLVAVKPKDSQEVLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPN
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCC
VNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGSGPAFVFS
CEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHH
FIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCHHHHHH
YLEEKGFKGTVMECINRTSQKAKKL
HHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHAIILTMRVGIVGFGNMGQAFALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDVK
CCEEEEEEEHHHEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
FLADNSDVVLVAVKPKDSQEVLQKLKDYKGIILSIMAGVSIEKMEKILGKDKKIVRVMPN
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCC
VNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGSGPAFVFS
CEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHH
FIDALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVTSPGGTTIEGIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCHHHHHH
YLEEKGFKGTVMECINRTSQKAKKL
HHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320