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The map label for this gene is leuB

Identifier: 15603826

GI number: 15603826

Start: 2200386

End: 2201465

Strand: Reverse

Name: leuB

Synonym: PM1961

Alternate gene names: 15603826

Gene position: 2201465-2200386 (Counterclockwise)

Preceding gene: 15603827

Following gene: 15603825

Centisome position: 97.52

GC content: 45.46

Gene sequence:

>1080_bases
ATGCAAACATTCAACATCGCGGTATTAAGTGGCGACGGTATCGGACCAGAAATCATAGCAGAAGCCATTAAAGTCTTAGA
CGTGGTACAACAAAAATACGCATTCAAATTAAACTATCGTACTTTTGATGTGGGTGGCATTGCCATCGACAACCACGGTA
CACCACTACCTGAAGCGACCTTAAAAGGCTGTGAAGAAAGCGATGCGATTTTATTTGGCTCGGTAGGCGGACCAAAATGG
GAACATTTGCCACCAGCACAACAACCCGAACGAGGCGCCTTATTGCCACTACGCAAACACTTTGCCTTATTCTGTAACTT
ACGTCCAGCCACCCTTTACAAAGGGTTAGAAAAATTCTGTCCATTGCGTGCCGATATTTCTGCCAAAGGCTTTGATATGG
TCACTGTGCGTGAATTAACCGGTGGCATTTATTTTGGTCAACCGAAAGGACGTGAAGGCGAAGGTGCCAATGAAAAAGCC
TTTGATACGGAAGTGTATCATCGTTATGAAATTGAACGTATTGCTCGCGTTGCTTTTGAAACTGCAATGAAACGTAGCAA
ACACGTGACGTCCGTAGATAAAGCCAATGTGTTGATCAGTTCTGTCTTATGGCGTGAAGTGGTATGTGACGTGGCGAAAG
ATTACCCTGAGGTGACATTAGATCATATCTACATTGATAACGCTACTATGCAATTAATCAAACAACCTGAATTTTTCGAT
GTGTTACTCTGTTCGAATATTTTCGGCGATATTATTTCCGATGAATGTGCGATGATCACGGGTTCAATGGGGATGTTGCC
TTCTGCCAGCCTGAACGAAAAAGGCTTTGGTTTATACGAACCTGCGGGTGGCTCCGCGCCTGATATCGCAGGCAAAGGCA
TCGCTAACCCGATTGCACAAATTTTATCGGCTGCCATGATGCTACGCCACAGTTTCAACTTAAACGACGCCGCGACAGCC
ATTGAAAACGCTGTGAAAAATGTCCTCGCAGAAGGACACCGCACCGCCGATTTAGCCGATGAAAGCCAACCACTTTCAAC
CAAACAAATGGGCGACCTCATCGCCCAAGCGGTAGAATAA

Upstream 100 bases:

>100_bases
TTCAAAACACACCGCACTTTAAACAGACATGATGCCAACATGTGATAAGAAGAAATTCATCCAAGACAAACTTCATTAAA
AGATCAAAAGGAAAAACCAA

Downstream 100 bases:

>100_bases
AACTATAAAAAAATTATACGTAATCGTTCTTAACGATTACTATGGCTTCGCCACCGTTGGCAAAGCCAATGTTCAAAAAC
TAAAGTTTTTGTCACCGCAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEATLKGCEESDAILFGSVGGPKW
EHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFCPLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKA
FDTEVYHRYEIERIARVAFETAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFD
VLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRHSFNLNDAATA
IENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE

Sequences:

>Translated_359_residues
MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEATLKGCEESDAILFGSVGGPKW
EHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFCPLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKA
FDTEVYHRYEIERIARVAFETAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFD
VLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRHSFNLNDAATA
IENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE
>Mature_359_residues
MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEATLKGCEESDAILFGSVGGPKW
EHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFCPLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKA
FDTEVYHRYEIERIARVAFETAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFD
VLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRHSFNLNDAATA
IENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=31.3782991202346, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI28178816, Length=327, Percent_Identity=29.0519877675841, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI28178821, Length=327, Percent_Identity=29.0519877675841, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4758582, Length=325, Percent_Identity=28.6153846153846, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI28178838, Length=325, Percent_Identity=28.6153846153846, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI28178819, Length=165, Percent_Identity=35.7575757575758, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=71.9887955182073, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1788101, Length=368, Percent_Identity=38.5869565217391, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=25.8706467661692, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=336, Percent_Identity=32.1428571428571, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=30.8571428571429, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=30.1724137931034, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=337, Percent_Identity=29.9703264094956, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=45.7534246575342, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=364, Percent_Identity=34.0659340659341, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=32.3615160349854, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=31.2138728323699, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=344, Percent_Identity=33.1395348837209, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24643268, Length=344, Percent_Identity=33.1395348837209, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=32.378223495702, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=27.5147928994083, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078633, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=27.5964391691395, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=25.5882352941176, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=25.5882352941176, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI20130355, Length=348, Percent_Identity=22.4137931034483, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_PASMU (Q9CJN6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246900.1
- ProteinModelPortal:   Q9CJN6
- SMR:   Q9CJN6
- GeneID:   1245308
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1961
- NMPDR:   fig|272843.1.peg.1962
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   PMUL272843:PM1961-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39062; Mature: 39062

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 98-98 BINDING 108-108 BINDING 137-137 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEAT
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEECCEEECCCCCCCCHHH
LKGCEESDAILFGSVGGPKWEHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFC
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHEEHCCCCHHHHHHHHHHHC
PLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKAFDTEVYHRYEIERIARVAFE
CCCCCCCCCCCCEEEHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCHHH
VLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQ
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHH
ILSAAMMLRHSFNLNDAATAIENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQTFNIAVLSGDGIGPEIIAEAIKVLDVVQQKYAFKLNYRTFDVGGIAIDNHGTPLPEAT
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEECCEEECCCCCCCCHHH
LKGCEESDAILFGSVGGPKWEHLPPAQQPERGALLPLRKHFALFCNLRPATLYKGLEKFC
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHEEHCCCCHHHHHHHHHHHC
PLRADISAKGFDMVTVRELTGGIYFGQPKGREGEGANEKAFDTEVYHRYEIERIARVAFE
CCCCCCCCCCCCEEEHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAMKRSKHVTSVDKANVLISSVLWREVVCDVAKDYPEVTLDHIYIDNATMQLIKQPEFFD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCCCHHH
VLLCSNIFGDIISDECAMITGSMGMLPSASLNEKGFGLYEPAGGSAPDIAGKGIANPIAQ
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHH
ILSAAMMLRHSFNLNDAATAIENAVKNVLAEGHRTADLADESQPLSTKQMGDLIAQAVE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100