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The map label for this gene is leuD

Identifier: 15603824

GI number: 15603824

Start: 2197831

End: 2198436

Strand: Reverse

Name: leuD

Synonym: PM1959

Alternate gene names: 15603824

Gene position: 2198436-2197831 (Counterclockwise)

Preceding gene: 15603825

Following gene: 15603823

Centisome position: 97.38

GC content: 41.42

Gene sequence:

>606_bases
ATGCCTAAAGAATTTAAACAACACACGGGTATCGCCGTCCCCCTTGATGCCTCAAACGTGGATACCGATGCTATTATCCC
AAAACAGTTCTTACAAAAAGTCACTCGTATTGGTTTTGGTCAACACTTATTCCATGAATGGCGCTTTTTAGATGATGAAG
GAAAACAACCCAACCCAGACTTCGTGTTAAACTACCCACGTTATCAAGGTGCCAGTATTTTATTAGCCCGTGAGAATTTT
GGTTGTGGTTCCTCTCGCGAACACGCACCTTGGGCGCTCGATGATTATGGTATTCGTGTGATTATTGCGCCCAGTTTTGC
TGATATTTTTTATGGCAATAGCTTAAATAATCAGATGTTACCAATTCGCCTTAGCGATGAAGAAGTGGAAGAATTGTTCC
AGTTTGTCAATGCTAACGAAGGTGCAACCATTACGGTGGATCTAGAAACACAAAGAGTCAGCGCAAATAACAAAGTCTAT
TCCTTTGAAATCGACCCTTTCCGTCGCCATTGTTTGTTAAATGGGTTAGACAATATCGGACTAACTTTGCAACATGAAGC
AAAAATCGCAGAATACGAAAGCAACATCCCTGCTTTCTTACGCTAA

Upstream 100 bases:

>100_bases
CGATTTTGCCTGAGTGTAAGAAAATTTTCTTTAAACGAAGAAAAGCAAAATCGCAAGTACGGTACAACATAACAGAATCA
ACATGTAAGAGAGAAATCAC

Downstream 100 bases:

>100_bases
ATATGTTCAAGAGATGAAACAATCATCTCTTGAACTTTTTCCCACCCATTCATATTATTTTTTCCTTGTTATTTTATGTA
CAAAATAAATACCTAAAGGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MPKEFKQHTGIAVPLDASNVDTDAIIPKQFLQKVTRIGFGQHLFHEWRFLDDEGKQPNPDFVLNYPRYQGASILLARENF
GCGSSREHAPWALDDYGIRVIIAPSFADIFYGNSLNNQMLPIRLSDEEVEELFQFVNANEGATITVDLETQRVSANNKVY
SFEIDPFRRHCLLNGLDNIGLTLQHEAKIAEYESNIPAFLR

Sequences:

>Translated_201_residues
MPKEFKQHTGIAVPLDASNVDTDAIIPKQFLQKVTRIGFGQHLFHEWRFLDDEGKQPNPDFVLNYPRYQGASILLARENF
GCGSSREHAPWALDDYGIRVIIAPSFADIFYGNSLNNQMLPIRLSDEEVEELFQFVNANEGATITVDLETQRVSANNKVY
SFEIDPFRRHCLLNGLDNIGLTLQHEAKIAEYESNIPAFLR
>Mature_200_residues
PKEFKQHTGIAVPLDASNVDTDAIIPKQFLQKVTRIGFGQHLFHEWRFLDDEGKQPNPDFVLNYPRYQGASILLARENFG
CGSSREHAPWALDDYGIRVIIAPSFADIFYGNSLNNQMLPIRLSDEEVEELFQFVNANEGATITVDLETQRVSANNKVYS
FEIDPFRRHCLLNGLDNIGLTLQHEAKIAEYESNIPAFLR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=200, Percent_Identity=72.5, Blast_Score=318, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=53.8071065989848, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_PASMU (Q9CJN8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246898.1
- ProteinModelPortal:   Q9CJN8
- GeneID:   1245306
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1959
- NMPDR:   fig|272843.1.peg.1960
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   PMUL272843:PM1959-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22885; Mature: 22754

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEFKQHTGIAVPLDASNVDTDAIIPKQFLQKVTRIGFGQHLFHEWRFLDDEGKQPNPD
CCCHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCC
FVLNYPRYQGASILLARENFGCGSSREHAPWALDDYGIRVIIAPSFADIFYGNSLNNQML
EEEECCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCHHHHEECCCCCCCEE
PIRLSDEEVEELFQFVNANEGATITVDLETQRVSANNKVYSFEIDPFRRHCLLNGLDNIG
EEEECHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEECCHHHHHHHHCCCCCCC
LTLQHEAKIAEYESNIPAFLR
EEEECCCHHHHHCCCCCHHCC
>Mature Secondary Structure 
PKEFKQHTGIAVPLDASNVDTDAIIPKQFLQKVTRIGFGQHLFHEWRFLDDEGKQPNPD
CCHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCC
FVLNYPRYQGASILLARENFGCGSSREHAPWALDDYGIRVIIAPSFADIFYGNSLNNQML
EEEECCCCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEECCCHHHHEECCCCCCCEE
PIRLSDEEVEELFQFVNANEGATITVDLETQRVSANNKVYSFEIDPFRRHCLLNGLDNIG
EEEECHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEEECCHHHHHHHHCCCCCCC
LTLQHEAKIAEYESNIPAFLR
EEEECCCHHHHHCCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100