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The map label for this gene is folD

Identifier: 15603798

GI number: 15603798

Start: 2171066

End: 2171920

Strand: Direct

Name: folD

Synonym: PM1933

Alternate gene names: 15603798

Gene position: 2171066-2171920 (Clockwise)

Preceding gene: 15603797

Following gene: 15603799

Centisome position: 96.17

GC content: 44.21

Gene sequence:

>855_bases
ATGACTGCACAAGTGATTTCTGGCACAGAACTCTCGAAAACAATTAAATCCCAAGTAGCACAAAAAATCGAAACCTATAC
TCAGCAGGGCAAACGCTCACCGGGTTTAGCGGTGATTCTCGTGGGGGCGGATCCGGCTTCTCAAGTCTATGTAGGAAGTA
AGCGTAAAAGTTGTGCAGAAATTGGGATCCAGTCAAAATCCTATGATTTACCTGAAACCACACAAGAAAGTGAATTATTG
GCATTAATTGATGAATTAAATGCAGATACGACGGTAGATGGGATTTTAGTACAATTACCACTTCCTAAACATATTGATAG
CACAAAAGTGATTGAACGTATTACGCCTGAAAAAGATGTAGATGGTTTTCATCCTTATAATGTGGGGCGTTTATGTCAAC
GTATTCCGACTTTACGTGCTTGCACGCCTTATGGTGTGATGAAATTGCTCGAAACCACGGGGATTGATTTACACGGTAAG
CATGCAGTGATTGTCGGTGCATCAAATATAGTGGGACGCCCGATGGCACTTGAGTTGTTATTGGCAGGCTGTACTGTCAC
CGTAACGCATCGTTTTACCAAAGATCTCGCACATCACGTTCGTCAAGCGGATATTCTTGTGGTTGCGGTGGGCAAACCGA
AGTTTATTCCGGGCGATTGGATCAAGGCGGGGGCAATTGTGATTGATGTCGGGATTAACCGTCAGGAAGGGAAATTAGTC
GGCGATGTGGAATACGATGTGGCACTTGAAAAAGCAGGATACATTACGCCCGTTCCTGGCGGTGTGGGACCCATGACGGT
CGCAATGTTAATGTTTAATACCTTATCCGCTTATGAAATGAAAATAGCCTCTTAG

Upstream 100 bases:

>100_bases
TCAATCAAACGCTTAAATTTATCTCAATTTGACTTACATTTACAGGCGTTTTTTGCGAGAATAGCAAACGTTTTCTTTCA
CGTAAATAAAGGATAAAGTC

Downstream 100 bases:

>100_bases
AATTGGTGTAGCAAAGTGCGGTTGACTTTAAGTAAAAATTCACCGCACTTTTATAAGTGGATTGTTCTTTTGCTCTTAAA
GATTGCGCGTGTCTTTTTGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQVISGTELSKTIKSQVAQKIETYTQQGKRSPGLAVILVGADPASQVYVGSKRKSCAEIGIQSKSYDLPETTQESELL
ALIDELNADTTVDGILVQLPLPKHIDSTKVIERITPEKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMALELLLAGCTVTVTHRFTKDLAHHVRQADILVVAVGKPKFIPGDWIKAGAIVIDVGINRQEGKLV
GDVEYDVALEKAGYITPVPGGVGPMTVAMLMFNTLSAYEMKIAS

Sequences:

>Translated_284_residues
MTAQVISGTELSKTIKSQVAQKIETYTQQGKRSPGLAVILVGADPASQVYVGSKRKSCAEIGIQSKSYDLPETTQESELL
ALIDELNADTTVDGILVQLPLPKHIDSTKVIERITPEKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMALELLLAGCTVTVTHRFTKDLAHHVRQADILVVAVGKPKFIPGDWIKAGAIVIDVGINRQEGKLV
GDVEYDVALEKAGYITPVPGGVGPMTVAMLMFNTLSAYEMKIAS
>Mature_283_residues
TAQVISGTELSKTIKSQVAQKIETYTQQGKRSPGLAVILVGADPASQVYVGSKRKSCAEIGIQSKSYDLPETTQESELLA
LIDELNADTTVDGILVQLPLPKHIDSTKVIERITPEKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKH
AVIVGASNIVGRPMALELLLAGCTVTVTHRFTKDLAHHVRQADILVVAVGKPKFIPGDWIKAGAIVIDVGINRQEGKLVG
DVEYDVALEKAGYITPVPGGVGPMTVAMLMFNTLSAYEMKIAS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=48.4536082474227, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=46.3917525773196, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=45.7044673539519, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI36796743, Length=213, Percent_Identity=30.0469483568075, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=68.3453237410072, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=43.728813559322, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=284, Percent_Identity=45.7746478873239, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=42.3841059602649, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=317, Percent_Identity=26.4984227129338, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=47.4226804123711, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=47.4226804123711, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=44.7552447552448, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=44.7552447552448, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=44.7552447552448, Blast_Score=230, Evalue=9e-61,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=44.7552447552448, Blast_Score=230, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PASMU (Q9CJR1)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246872.1
- HSSP:   P11586
- ProteinModelPortal:   Q9CJR1
- SMR:   Q9CJR1
- GeneID:   1245280
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1933
- NMPDR:   fig|272843.1.peg.1934
- HOGENOM:   HBG328751
- OMA:   GIDNTKV
- ProtClustDB:   PRK10792
- BioCyc:   PMUL272843:PM1933-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30539; Mature: 30408

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQVISGTELSKTIKSQVAQKIETYTQQGKRSPGLAVILVGADPASQVYVGSKRKSCAE
CCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCHHHHH
IGIQSKSYDLPETTQESELLALIDELNADTTVDGILVQLPLPKHIDSTKVIERITPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMALELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHH
LAGCTVTVTHRFTKDLAHHVRQADILVVAVGKPKFIPGDWIKAGAIVIDVGINRQEGKLV
HHCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEECCEEEEEECCCCCCCCEE
GDVEYDVALEKAGYITPVPGGVGPMTVAMLMFNTLSAYEMKIAS
ECEEHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TAQVISGTELSKTIKSQVAQKIETYTQQGKRSPGLAVILVGADPASQVYVGSKRKSCAE
CCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCHHHHH
IGIQSKSYDLPETTQESELLALIDELNADTTVDGILVQLPLPKHIDSTKVIERITPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMALELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHH
LAGCTVTVTHRFTKDLAHHVRQADILVVAVGKPKFIPGDWIKAGAIVIDVGINRQEGKLV
HHCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEECCEEEEEECCCCCCCCEE
GDVEYDVALEKAGYITPVPGGVGPMTVAMLMFNTLSAYEMKIAS
ECEEHEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100