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The map label for this gene is rlmH

Identifier: 15603788

GI number: 15603788

Start: 2162163

End: 2162630

Strand: Direct

Name: rlmH

Synonym: PM1923

Alternate gene names: 15603788

Gene position: 2162163-2162630 (Clockwise)

Preceding gene: 15603787

Following gene: 15603789

Centisome position: 95.78

GC content: 46.58

Gene sequence:

>468_bases
GTGAAGATTCAACTGATTGCCGTGGGTACCAAAATGCCGAACTGGGTGACGATGGGATTTGAAGAATATCAACGTCGTTT
TCCCAAAGAGATGCCTTTTGAGCTTATTGAAATTCCAGCCGGAAAACGCGGTAAAAATGCGGATATTAAACGTATTTTAG
AACAAGAAGGCAAAGCGATGTTAGCCGCGTGTGGAAAAAGCCGTATCGTCACGTTAGATATTCCCGGTAAACCTTGGACT
ACGGAGCAATTGGCTACGCAGCTAGAGGTCTGGAAAAACGATGGGCGTGATCTCAGTTTACTGATTGGTGGTCCAGAAGG
GTTGTCTGCCGAATGTAAAGCAGCAGCAGAACAAAGTTGGTCATTATCACCGCTTACGCTACCGCATCCGCTCGTGCGTG
TTGTGGTCGCCGAGAGTTTGTATCGTGCTTGGTCTGTGACCATGAATCATCCATATCATCGTGAATAA

Upstream 100 bases:

>100_bases
GGCAGATTGGATCGTCGTTGATTTAGGGCAAGCGATTGTGCATATTATGCAAGCAGACAGTCGAGAGATGTATCAGCTTG
AGAAACTATGGGGCTAATCA

Downstream 100 bases:

>100_bases
TGCTGTGATTCAATGAAAAAGTTAAAAAAATTGTTGGCTAAACCACAACAAGAATTGATTAGAGATAAAAAATCGGAGCG
CAATTTATTTGCCCGCCGAG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKIQLIAVGTKMPNWVTMGFEEYQRRFPKEMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKSRIVTLDIPGKPWT
TEQLATQLEVWKNDGRDLSLLIGGPEGLSAECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTMNHPYHRE

Sequences:

>Translated_155_residues
MKIQLIAVGTKMPNWVTMGFEEYQRRFPKEMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKSRIVTLDIPGKPWT
TEQLATQLEVWKNDGRDLSLLIGGPEGLSAECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTMNHPYHRE
>Mature_155_residues
MKIQLIAVGTKMPNWVTMGFEEYQRRFPKEMPFELIEIPAGKRGKNADIKRILEQEGKAMLAACGKSRIVTLDIPGKPWT
TEQLATQLEVWKNDGRDLSLLIGGPEGLSAECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTMNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=82.5806451612903, Blast_Score=269, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PASMU (Q9CJR9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246862.1
- ProteinModelPortal:   Q9CJR9
- SMR:   Q9CJR9
- GeneID:   1245270
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1923
- NMPDR:   fig|272843.1.peg.1924
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   PMUL272843:PM1923-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17441; Mature: 17441

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLIAVGTKMPNWVTMGFEEYQRRFPKEMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEEECCCCCCEEEECHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAACGKSRIVTLDIPGKPWTTEQLATQLEVWKNDGRDLSLLIGGPEGLSAECKAAAEQSW
EECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPLVRVVVAESLYRAWSVTMNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MKIQLIAVGTKMPNWVTMGFEEYQRRFPKEMPFELIEIPAGKRGKNADIKRILEQEGKAM
CEEEEEEECCCCCCEEEECHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAACGKSRIVTLDIPGKPWTTEQLATQLEVWKNDGRDLSLLIGGPEGLSAECKAAAEQSW
EECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCC
SLSPLTLPHPLVRVVVAESLYRAWSVTMNHPYHRE
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100