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The map label for this gene is purB [H]

Identifier: 15603716

GI number: 15603716

Start: 2091547

End: 2092914

Strand: Direct

Name: purB [H]

Synonym: PM1851

Alternate gene names: 15603716

Gene position: 2091547-2092914 (Clockwise)

Preceding gene: 15603715

Following gene: 15603717

Centisome position: 92.65

GC content: 39.4

Gene sequence:

>1368_bases
ATGCAACTTAATGCATTAACTGCACTTTCCCCGATTGATGGTCGTTATCAAGACAAAGTGAGTTCACTACGTTCAATTTT
TAGTGAATTTGGCTTGTTAAAATTTCGTGTGACAGTGGAAGTGCGCTGGTTACAAAAATTAGCTGCCACCGATCAAATTC
AAGAAGTTTCTTTATTATCTCAAGAGGCAAACGATTACTTGAATCACATCGTTGAAAATTTCAGTGTGCAAGATGCCGAA
CGTATTAAAGAAATTGAACGCACGACGAACCACGATGTGAAAGCAGTGGAATATTTCTTAAAAGAAAAAAGTGAAGCTTT
ACCAGAATTAGCCAAAATCAGTGAATTTATTCACTTCGCTTGTACCTCAGAAGACATTAACAATCTTTCGCATGCATTAA
TGCTAAAAACGGCACGTGAAGAGGTGATTCTTCCAGAATGGCAAAACTTAATTGCGGATATCAGCGCACTTGCTAATGAA
TATAAAACTATTCCGTTACTGTCTCGTACACATGGTCAACCTGCATCACCTTCTACTATTGGGAAAGAAATGGCAAACGT
GGTGTATCGCTTACAACGCCAGTTTAAACAATTACAGCAGATTGACATTTTAGGTAAAATCAATGGTGCGGTAGGTAACT
ATAATGCCCATTTATCAGCGTATCCTGATATTGATTGGCATCAATTCAGTGAAGAGTTTGTGACGTCATTAGGTTTAACT
TGGAACCCATATACCACACAAATTGAACCACATGACTACATTGCTGAATTTTTCGATAATGTGGCGCGCTTTAATACTAT
TATTATCGATTTTGACCGTGACTTATGGGGATATATTGCGTTAAATCATTTCAAACAACGTACCATTGCCGGTGAAATTG
GATCTTCAACCATGCCACATAAAGTCAATCCAATCGACTTTGAAAATTCAGAGGGAAATCTTGGTTTAGCCAATGCAGTC
ATGGCACATTTAGGCGCAAAATTACCGGTTTCTCGCTGGCAACGTGATTTAACGGATTCCACCGTATTACGTAACTTAGG
TGTGGGATTAGGCTATTGTTTAATTGCGTACGCGGCGACACGTAAAGGTATCAGTAAATTAGAAGTGAACGAGCAGCATT
TACGTGATGAGTTGGATCAAAACTGGGAAGTCCTTGCCGAACCTATCCAAACAGTGATGCGTCGTTATGGTATTGAAAAG
CCGTATGAGAAATTAAAAGCGTTAACCCGTGGTAAGCGTGTGGATGCAGAAGCGATGCGTGAATTTATCGAAACATTAGA
TATTCCAGCGCAAGAAAAAATCCGTTTACAACAAATGACGCCTGCAACCTATATTGGTGCCGCGGTGGAATTAGTGGAAA
AACTGTAA

Upstream 100 bases:

>100_bases
AACAAAATGGCAAGTGTTATTCTCTCGTCGCAAAATTATCGCTATGGCAAAACAGATTTATTCTTCACTTTAATCATTTT
ATTTTATGGAGCAAACCGCT

Downstream 100 bases:

>100_bases
CATAAAATAAATAGAGTGAGTCAAAAAATAAGGCTGTGTGATATGTCATACAGCCTTATTTATTTTAACTAATAAATTAA
ATTATTTCTGATATAAAAAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQLNALTALSPIDGRYQDKVSSLRSIFSEFGLLKFRVTVEVRWLQKLAATDQIQEVSLLSQEANDYLNHIVENFSVQDAE
RIKEIERTTNHDVKAVEYFLKEKSEALPELAKISEFIHFACTSEDINNLSHALMLKTAREEVILPEWQNLIADISALANE
YKTIPLLSRTHGQPASPSTIGKEMANVVYRLQRQFKQLQQIDILGKINGAVGNYNAHLSAYPDIDWHQFSEEFVTSLGLT
WNPYTTQIEPHDYIAEFFDNVARFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MAHLGAKLPVSRWQRDLTDSTVLRNLGVGLGYCLIAYAATRKGISKLEVNEQHLRDELDQNWEVLAEPIQTVMRRYGIEK
PYEKLKALTRGKRVDAEAMREFIETLDIPAQEKIRLQQMTPATYIGAAVELVEKL

Sequences:

>Translated_455_residues
MQLNALTALSPIDGRYQDKVSSLRSIFSEFGLLKFRVTVEVRWLQKLAATDQIQEVSLLSQEANDYLNHIVENFSVQDAE
RIKEIERTTNHDVKAVEYFLKEKSEALPELAKISEFIHFACTSEDINNLSHALMLKTAREEVILPEWQNLIADISALANE
YKTIPLLSRTHGQPASPSTIGKEMANVVYRLQRQFKQLQQIDILGKINGAVGNYNAHLSAYPDIDWHQFSEEFVTSLGLT
WNPYTTQIEPHDYIAEFFDNVARFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MAHLGAKLPVSRWQRDLTDSTVLRNLGVGLGYCLIAYAATRKGISKLEVNEQHLRDELDQNWEVLAEPIQTVMRRYGIEK
PYEKLKALTRGKRVDAEAMREFIETLDIPAQEKIRLQQMTPATYIGAAVELVEKL
>Mature_455_residues
MQLNALTALSPIDGRYQDKVSSLRSIFSEFGLLKFRVTVEVRWLQKLAATDQIQEVSLLSQEANDYLNHIVENFSVQDAE
RIKEIERTTNHDVKAVEYFLKEKSEALPELAKISEFIHFACTSEDINNLSHALMLKTAREEVILPEWQNLIADISALANE
YKTIPLLSRTHGQPASPSTIGKEMANVVYRLQRQFKQLQQIDILGKINGAVGNYNAHLSAYPDIDWHQFSEEFVTSLGLT
WNPYTTQIEPHDYIAEFFDNVARFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAV
MAHLGAKLPVSRWQRDLTDSTVLRNLGVGLGYCLIAYAATRKGISKLEVNEQHLRDELDQNWEVLAEPIQTVMRRYGIEK
PYEKLKALTRGKRVDAEAMREFIETLDIPAQEKIRLQQMTPATYIGAAVELVEKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=77.1428571428572, Blast_Score=751, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51806; Mature: 51806

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNALTALSPIDGRYQDKVSSLRSIFSEFGLLKFRVTVEVRWLQKLAATDQIQEVSLLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEANDYLNHIVENFSVQDAERIKEIERTTNHDVKAVEYFLKEKSEALPELAKISEFIHFA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLKTAREEVILPEWQNLIADISALANEYKTIPLLSRTHGQPASPSTI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHH
GKEMANVVYRLQRQFKQLQQIDILGKINGAVGNYNAHLSAYPDIDWHQFSEEFVTSLGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDNVARFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMAHLGAKLPVSRWQRDLTDSTVLRNLGVGLGYCLIAYAAT
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNEQHLRDELDQNWEVLAEPIQTVMRRYGIEKPYEKLKALTRGKRVDAEAMR
HHCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
EFIETLDIPAQEKIRLQQMTPATYIGAAVELVEKL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MQLNALTALSPIDGRYQDKVSSLRSIFSEFGLLKFRVTVEVRWLQKLAATDQIQEVSLLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
QEANDYLNHIVENFSVQDAERIKEIERTTNHDVKAVEYFLKEKSEALPELAKISEFIHFA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLSHALMLKTAREEVILPEWQNLIADISALANEYKTIPLLSRTHGQPASPSTI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHH
GKEMANVVYRLQRQFKQLQQIDILGKINGAVGNYNAHLSAYPDIDWHQFSEEFVTSLGLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAEFFDNVARFNTIIIDFDRDLWGYIALNHFKQRTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLANAVMAHLGAKLPVSRWQRDLTDSTVLRNLGVGLGYCLIAYAAT
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKGISKLEVNEQHLRDELDQNWEVLAEPIQTVMRRYGIEKPYEKLKALTRGKRVDAEAMR
HHCHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
EFIETLDIPAQEKIRLQQMTPATYIGAAVELVEKL
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]