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The map label for this gene is rfaF [H]

Identifier: 15603709

GI number: 15603709

Start: 2083980

End: 2085026

Strand: Reverse

Name: rfaF [H]

Synonym: PM1844

Alternate gene names: 15603709

Gene position: 2085026-2083980 (Counterclockwise)

Preceding gene: 15603710

Following gene: 15603708

Centisome position: 92.36

GC content: 43.94

Gene sequence:

>1047_bases
ATGCATATTTTAATTATTGGACCCTCTTGGGTTGGCGATATGATGATGTCGCATAGCCTGTATCAAACACTCAAACAACA
CTATCCCCTGTGTGAAATTGATGTCATGGCACCCAATTGGTGTAAACCTCTCTTAGAACGTATGCCTGAAGTGCGACAAG
CCATTACAATGCCATTAGGACATGGGGCATTTGCCTTGGGTGATCGTTATCGCTTAGGCAAAGCATTACGCCACCAATAT
GATATGGCGATTGTTCTACCTAATTCACTCAAATCCGCATTCATTCCTTTTTTTGCCAAGATTCCGCTTCGCCGTGGTTG
GAAAGGCGAAAGTCGCTATCTCTTTTTAAATGATTTACGCAGTAATAAAAAAGACTATCCGATGATGGTGCAACGCTATA
TTGCTTTAGCTTTTGAAAAAGGTGCTGTGCCGAAAGCAGAGGATTTGCCACGACCCGTCCCCTATTTAACGGTCGATCCC
GTCACACAACAACATACCTTAAAAAAATTTGAAAAACAGACCGCACTTATTTCCCCACGCCCGAGTATTGGCTTTTGCCC
GGGGGCAGAATTTGGTCCCGCAAAACGCTGGCCTCATTATCATTATGCTAAATTGGCAGAAATGCTGATTCAACAAGGCT
ATGCCATTCGTTTGTTTGGTTCTGCCAAAGATGAAGCCGTAGGCGAAGAAATCCGCCAAGCGCTACCTGAAACAATGCGT
CACTTTTGCATAAATCTGGCAGGGCAAACCAGTTTGAATGAGGCTGTGGATTTAATCGCTGATTGTACCGCTATCGTCAC
CAATGACAGTGGACTCATGCATATCGCAGCCGCAGTTCAGCGCCCTTTAGTGGCACTTTATGGACCAACGAGCCCCACTT
ATACGCCTCCACTTTCTGAAAAAGCCGTAATTATTCGTTTAATTGAAGGTGGCTTAATCAAAGTGCGTAAAGGCAAAGAC
AGTGACGAAGGTTACCATCAAAGTTTAATTGATATTCAACCGGAATTGGTATTCGATAAACTCACAGGATTACTTGCTAA
CACATGA

Upstream 100 bases:

>100_bases
TGCATATTGAGGCAAAAATTATTCAATACCAATAAATCACCTCAGAAATCGTCTAAAATACGCGATATGATTTGTTATTT
TCAACATGGATAGAATAAGT

Downstream 100 bases:

>100_bases
AAATTTGTTTAGTCAAAACCTCATCAATGGGCGATATTCTGCACAGTTTACCTGCGCTCACCGATGCCCAACGCGCCCTC
CCCAATTTACAAGTTGATTG

Product: RfaF

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MHILIIGPSWVGDMMMSHSLYQTLKQHYPLCEIDVMAPNWCKPLLERMPEVRQAITMPLGHGAFALGDRYRLGKALRHQY
DMAIVLPNSLKSAFIPFFAKIPLRRGWKGESRYLFLNDLRSNKKDYPMMVQRYIALAFEKGAVPKAEDLPRPVPYLTVDP
VTQQHTLKKFEKQTALISPRPSIGFCPGAEFGPAKRWPHYHYAKLAEMLIQQGYAIRLFGSAKDEAVGEEIRQALPETMR
HFCINLAGQTSLNEAVDLIADCTAIVTNDSGLMHIAAAVQRPLVALYGPTSPTYTPPLSEKAVIIRLIEGGLIKVRKGKD
SDEGYHQSLIDIQPELVFDKLTGLLANT

Sequences:

>Translated_348_residues
MHILIIGPSWVGDMMMSHSLYQTLKQHYPLCEIDVMAPNWCKPLLERMPEVRQAITMPLGHGAFALGDRYRLGKALRHQY
DMAIVLPNSLKSAFIPFFAKIPLRRGWKGESRYLFLNDLRSNKKDYPMMVQRYIALAFEKGAVPKAEDLPRPVPYLTVDP
VTQQHTLKKFEKQTALISPRPSIGFCPGAEFGPAKRWPHYHYAKLAEMLIQQGYAIRLFGSAKDEAVGEEIRQALPETMR
HFCINLAGQTSLNEAVDLIADCTAIVTNDSGLMHIAAAVQRPLVALYGPTSPTYTPPLSEKAVIIRLIEGGLIKVRKGKD
SDEGYHQSLIDIQPELVFDKLTGLLANT
>Mature_348_residues
MHILIIGPSWVGDMMMSHSLYQTLKQHYPLCEIDVMAPNWCKPLLERMPEVRQAITMPLGHGAFALGDRYRLGKALRHQY
DMAIVLPNSLKSAFIPFFAKIPLRRGWKGESRYLFLNDLRSNKKDYPMMVQRYIALAFEKGAVPKAEDLPRPVPYLTVDP
VTQQHTLKKFEKQTALISPRPSIGFCPGAEFGPAKRWPHYHYAKLAEMLIQQGYAIRLFGSAKDEAVGEEIRQALPETMR
HFCINLAGQTSLNEAVDLIADCTAIVTNDSGLMHIAAAVQRPLVALYGPTSPTYTPPLSEKAVIIRLIEGGLIKVRKGKD
SDEGYHQSLIDIQPELVFDKLTGLLANT

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=349, Percent_Identity=62.1776504297994, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39065; Mature: 39065

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILIIGPSWVGDMMMSHSLYQTLKQHYPLCEIDVMAPNWCKPLLERMPEVRQAITMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
HGAFALGDRYRLGKALRHQYDMAIVLPNSLKSAFIPFFAKIPLRRGWKGESRYLFLNDLR
CCCHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHH
SNKKDYPMMVQRYIALAFEKGAVPKAEDLPRPVPYLTVDPVTQQHTLKKFEKQTALISPR
CCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCC
PSIGFCPGAEFGPAKRWPHYHYAKLAEMLIQQGYAIRLFGSAKDEAVGEEIRQALPETMR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
HFCINLAGQTSLNEAVDLIADCTAIVTNDSGLMHIAAAVQRPLVALYGPTSPTYTPPLSE
HHHHHHCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCC
KAVIIRLIEGGLIKVRKGKDSDEGYHQSLIDIQPELVFDKLTGLLANT
CEEEEEEECCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHILIIGPSWVGDMMMSHSLYQTLKQHYPLCEIDVMAPNWCKPLLERMPEVRQAITMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
HGAFALGDRYRLGKALRHQYDMAIVLPNSLKSAFIPFFAKIPLRRGWKGESRYLFLNDLR
CCCHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHH
SNKKDYPMMVQRYIALAFEKGAVPKAEDLPRPVPYLTVDPVTQQHTLKKFEKQTALISPR
CCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCC
PSIGFCPGAEFGPAKRWPHYHYAKLAEMLIQQGYAIRLFGSAKDEAVGEEIRQALPETMR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
HFCINLAGQTSLNEAVDLIADCTAIVTNDSGLMHIAAAVQRPLVALYGPTSPTYTPPLSE
HHHHHHCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCC
KAVIIRLIEGGLIKVRKGKDSDEGYHQSLIDIQPELVFDKLTGLLANT
CEEEEEEECCCEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]