Click here to switch to the map view.

The map label for this gene is nfnB [C]

Identifier: 15603697

GI number: 15603697

Start: 2070484

End: 2071152

Strand: Reverse

Name: nfnB [C]

Synonym: PM1832

Alternate gene names: 15603697

Gene position: 2071152-2070484 (Counterclockwise)

Preceding gene: 15603698

Following gene: 15603696

Centisome position: 91.75

GC content: 43.65

Gene sequence:

>669_bases
ATGTCCATTTCAAAAACAGATATCCTCAATGCCTTTCATTTTCGTCACGCTTGCAAAGCCTATGATCCAAGTAAGAAAAT
CACTGAAGAGGATTTCCGTTTTATTTTAGAAACGGGTCGCCTTTCGCCGAGCTCATTTGGTTTCGAGCCGTGGAAGTTCT
TAGTGATTGAAAACCCCGACATCAAAGCATTGATCCGTGATACGGCTTGGGGAGCGAAAGATAAAGTGATGGATTGTAGT
CATTTTGTGGTGGTGTTAGTGCGCCAACCAGAGACCTTACTTGCTGATGGTGAATATGTGCAACATATGATGCGAGATGT
CCATCATATCCCCGAAGAAATGCGCAAAATCCGTCAAAATTTCTACCGCACGTTTAGCGAACAGGATTTTGCCTTAACTG
AGAATCCAAAAGCTTTTTACGATTGGGCGTGTCGTCAAAGCTATATTGCCCTGGCAAATATGCTGACCAGTGCGGCAATG
ATCGGCATCGACTCAACCCCAATTGAAGGTTTCCCATTGGAAAAAATGGATCAAGCGTTTGTCGAACAAGGACTTTATGA
TACGAAAACCTATAAACTGAGTGTGATGATCGCCTTTGGTTACCGTTTAAACGAGCCAAAAGCCAAAACACGCCAAGCCT
TTGACGATGTGGTGGAGTGGGTAAGATAA

Upstream 100 bases:

>100_bases
TGTTTTGATACTTATTCCAGAGAACAGGCGAAACGCCAACAGTTCTCCCGAATTATTTAAACAAACTTTTTGAAAAACCA
CCGCACTTTGGGAGAAAACC

Downstream 100 bases:

>100_bases
CAACCCGCATTTTAGCGAAAGAAAAGCAAGAATAGGCGCTTATGGCGTCTATTTTTTATGGCTCTTGAAAACTAAACTTT
GGCTATTTTTTCCCCTTCCC

Product: hypothetical protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSISKTDILNAFHFRHACKAYDPSKKITEEDFRFILETGRLSPSSFGFEPWKFLVIENPDIKALIRDTAWGAKDKVMDCS
HFVVVLVRQPETLLADGEYVQHMMRDVHHIPEEMRKIRQNFYRTFSEQDFALTENPKAFYDWACRQSYIALANMLTSAAM
IGIDSTPIEGFPLEKMDQAFVEQGLYDTKTYKLSVMIAFGYRLNEPKAKTRQAFDDVVEWVR

Sequences:

>Translated_222_residues
MSISKTDILNAFHFRHACKAYDPSKKITEEDFRFILETGRLSPSSFGFEPWKFLVIENPDIKALIRDTAWGAKDKVMDCS
HFVVVLVRQPETLLADGEYVQHMMRDVHHIPEEMRKIRQNFYRTFSEQDFALTENPKAFYDWACRQSYIALANMLTSAAM
IGIDSTPIEGFPLEKMDQAFVEQGLYDTKTYKLSVMIAFGYRLNEPKAKTRQAFDDVVEWVR
>Mature_221_residues
SISKTDILNAFHFRHACKAYDPSKKITEEDFRFILETGRLSPSSFGFEPWKFLVIENPDIKALIRDTAWGAKDKVMDCSH
FVVVLVRQPETLLADGEYVQHMMRDVHHIPEEMRKIRQNFYRTFSEQDFALTENPKAFYDWACRQSYIALANMLTSAAMI
GIDSTPIEGFPLEKMDQAFVEQGLYDTKTYKLSVMIAFGYRLNEPKAKTRQAFDDVVEWVR

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25842; Mature: 25711

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISKTDILNAFHFRHACKAYDPSKKITEEDFRFILETGRLSPSSFGFEPWKFLVIENPD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
IKALIRDTAWGAKDKVMDCSHFVVVLVRQPETLLADGEYVQHMMRDVHHIPEEMRKIRQN
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FYRTFSEQDFALTENPKAFYDWACRQSYIALANMLTSAAMIGIDSTPIEGFPLEKMDQAF
HHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VEQGLYDTKTYKLSVMIAFGYRLNEPKAKTRQAFDDVVEWVR
HHCCCCCCCEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SISKTDILNAFHFRHACKAYDPSKKITEEDFRFILETGRLSPSSFGFEPWKFLVIENPD
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
IKALIRDTAWGAKDKVMDCSHFVVVLVRQPETLLADGEYVQHMMRDVHHIPEEMRKIRQN
HHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FYRTFSEQDFALTENPKAFYDWACRQSYIALANMLTSAAMIGIDSTPIEGFPLEKMDQAF
HHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VEQGLYDTKTYKLSVMIAFGYRLNEPKAKTRQAFDDVVEWVR
HHCCCCCCCEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA