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The map label for this gene is ppx [H]

Identifier: 15603672

GI number: 15603672

Start: 2031177

End: 2032727

Strand: Reverse

Name: ppx [H]

Synonym: PM1807

Alternate gene names: 15603672

Gene position: 2032727-2031177 (Counterclockwise)

Preceding gene: 15603673

Following gene: 15603670

Centisome position: 90.04

GC content: 41.33

Gene sequence:

>1551_bases
ATGAAGAGTGAAAATGTCATGGAAAAAGGCACCGCACTTTCCATTCCCTCGCGTCTCGAAGCGAGGGAAATTGCTGCCAT
TGATTTGGGTTCAAATAGCTTTCATATGATTGTCGCACGTATTATGAATGGCTCTATTCAGATTCTTTCTCGTTTAAAGC
AAAAAGTCCAGCTCGCAGAGGGCTTAGATGAAAACAATGTTCTCAACCAAGCGGCAATTACTCGTGGCGTAAATTGTTTA
GCCTTGTTTGCGGAGCGCTTACAGGGGTTCGCACCAGAAAATGTCAATGTTGTTGGCACCTATACCTTACGTCGTGCAGT
GAACAATGACGAGTTTTTACGCCAAGCCAAAGCTGTTTTCCCTTATCCAATCAATATCATTACAGGGAAAACCGAAGCGC
AAACTATTTATGCCGGTGTGTCACACACCCAACCTGAAACTGGAAAAAAATTTGTCATTGATATTGGTGGTGGCTCGACT
GAAATGATTATTGGGGAAAATTTCACCCCTATTTTAGCTGAAAGTCGACATATGGGGTGTGTCAGCTTTGCGAAACAATT
TTTCCCTCAAGGTGAAATCAGTCAGTTACATTTTGAAAATGCTCGTCAAGCCGCATTAATGAAAATTGAAGACTTAGCCT
ATGAATATCGACATTTGGGCTGGGATGTGGTGTTAGGCTCCTCTGGTACAATTAAAACTGTACATCAAGTCATCATGGCA
AATATTGATCCAAACGGTCTTATCACCCCTGAACGTCTACAACAATTAATCAAACAAACATTACAGCATACCCACTTTGA
AGATCTGAAATTATCCGGCTTGATTGAAGAACGTGCTGATGTCTTTGTGCCCGGATTGGCTATTTTGAGTGCTGTATTTG
AAACATTTGGTATTCAACAAATGCGTTATTCTGATGGGGCGTTACGCGAAGGGGTCATGTATAGCCTCGAAAAGACCTTT
CAAGTAAGCAATATTCGTGAGCGGACGGCGTTAGGTTTAGCAGAACAATTTACCATAGACCAAGCCCAAGCAGAACGTGT
CCATCAAAGTGTTAAACTGCTTAGCCAACAATATCGCCATTGGAAAAAACCACAACTGGCAGCAGAGATGATCGAAATCG
TCCTGTGGGCGGCACGTTTACATGAAGTCGGCATTGTGATTAACCATAAAGGGATGCAAAAACACTCCGCCTATATTTTA
CAAAATATAGAATTACCAGGTTTTGATAAGGAACAACAACGCTTATTAGCCACCTTAGTGCGTTATCAAATTAACCTGTT
TAAACTGTCTGAATTAAGTAAATTTGCACGGTATGATGAACAAGATGTGTTAGCCTTAGTACGTTTATTACGCTTAGCCA
TTATCTTAAACAAATCCCGTCAAGCTACAGAGAAAACCGAGCAAATCACCTTGACGTTCAACCGTGCTTTAACCCATTGG
ACACTCAAGTTTGCACAAGGCTATTTGACGCATACACCGCTGATCTGTAACGAGCTAAAAGCCGAAAAACGCGTGCTTAA
TGAAATGGGATTAGATATCACGTTTAACTAA

Upstream 100 bases:

>100_bases
CGGTACGTTATCGCTTAATCCCTAAATTATATTTACAATCTGTTTCCGGTGTCACCCAAGCGGTGGATTTGCTATATCAA
TTTGAATTTTAAGGAAGAAT

Downstream 100 bases:

>100_bases
ACAAACCGCCCACCAACAGGTGGGCAGCAAGTACATAAATACTGATCGTAAATGCGGTTACTTTTTCGTTTGTTTAGTAG
GGCGTTGCCAACCTTGAATA

Product: hypothetical protein

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKSENVMEKGTALSIPSRLEAREIAAIDLGSNSFHMIVARIMNGSIQILSRLKQKVQLAEGLDENNVLNQAAITRGVNCL
ALFAERLQGFAPENVNVVGTYTLRRAVNNDEFLRQAKAVFPYPINIITGKTEAQTIYAGVSHTQPETGKKFVIDIGGGST
EMIIGENFTPILAESRHMGCVSFAKQFFPQGEISQLHFENARQAALMKIEDLAYEYRHLGWDVVLGSSGTIKTVHQVIMA
NIDPNGLITPERLQQLIKQTLQHTHFEDLKLSGLIEERADVFVPGLAILSAVFETFGIQQMRYSDGALREGVMYSLEKTF
QVSNIRERTALGLAEQFTIDQAQAERVHQSVKLLSQQYRHWKKPQLAAEMIEIVLWAARLHEVGIVINHKGMQKHSAYIL
QNIELPGFDKEQQRLLATLVRYQINLFKLSELSKFARYDEQDVLALVRLLRLAIILNKSRQATEKTEQITLTFNRALTHW
TLKFAQGYLTHTPLICNELKAEKRVLNEMGLDITFN

Sequences:

>Translated_516_residues
MKSENVMEKGTALSIPSRLEAREIAAIDLGSNSFHMIVARIMNGSIQILSRLKQKVQLAEGLDENNVLNQAAITRGVNCL
ALFAERLQGFAPENVNVVGTYTLRRAVNNDEFLRQAKAVFPYPINIITGKTEAQTIYAGVSHTQPETGKKFVIDIGGGST
EMIIGENFTPILAESRHMGCVSFAKQFFPQGEISQLHFENARQAALMKIEDLAYEYRHLGWDVVLGSSGTIKTVHQVIMA
NIDPNGLITPERLQQLIKQTLQHTHFEDLKLSGLIEERADVFVPGLAILSAVFETFGIQQMRYSDGALREGVMYSLEKTF
QVSNIRERTALGLAEQFTIDQAQAERVHQSVKLLSQQYRHWKKPQLAAEMIEIVLWAARLHEVGIVINHKGMQKHSAYIL
QNIELPGFDKEQQRLLATLVRYQINLFKLSELSKFARYDEQDVLALVRLLRLAIILNKSRQATEKTEQITLTFNRALTHW
TLKFAQGYLTHTPLICNELKAEKRVLNEMGLDITFN
>Mature_516_residues
MKSENVMEKGTALSIPSRLEAREIAAIDLGSNSFHMIVARIMNGSIQILSRLKQKVQLAEGLDENNVLNQAAITRGVNCL
ALFAERLQGFAPENVNVVGTYTLRRAVNNDEFLRQAKAVFPYPINIITGKTEAQTIYAGVSHTQPETGKKFVIDIGGGST
EMIIGENFTPILAESRHMGCVSFAKQFFPQGEISQLHFENARQAALMKIEDLAYEYRHLGWDVVLGSSGTIKTVHQVIMA
NIDPNGLITPERLQQLIKQTLQHTHFEDLKLSGLIEERADVFVPGLAILSAVFETFGIQQMRYSDGALREGVMYSLEKTF
QVSNIRERTALGLAEQFTIDQAQAERVHQSVKLLSQQYRHWKKPQLAAEMIEIVLWAARLHEVGIVINHKGMQKHSAYIL
QNIELPGFDKEQQRLLATLVRYQINLFKLSELSKFARYDEQDVLALVRLLRLAIILNKSRQATEKTEQITLTFNRALTHW
TLKFAQGYLTHTPLICNELKAEKRVLNEMGLDITFN

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=483, Percent_Identity=52.3809523809524, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI48994966, Length=472, Percent_Identity=40.8898305084746, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 58283; Mature: 58283

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSENVMEKGTALSIPSRLEAREIAAIDLGSNSFHMIVARIMNGSIQILSRLKQKVQLAE
CCCCCHHHCCCEECCCCCCCHHHEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GLDENNVLNQAAITRGVNCLALFAERLQGFAPENVNVVGTYTLRRAVNNDEFLRQAKAVF
CCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHCC
PYPINIITGKTEAQTIYAGVSHTQPETGKKFVIDIGGGSTEMIIGENFTPILAESRHMGC
CCCEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCCCCHH
VSFAKQFFPQGEISQLHFENARQAALMKIEDLAYEYRHLGWDVVLGSSGTIKTVHQVIMA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
NIDPNGLITPERLQQLIKQTLQHTHFEDLKLSGLIEERADVFVPGLAILSAVFETFGIQQ
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHH
MRYSDGALREGVMYSLEKTFQVSNIRERTALGLAEQFTIDQAQAERVHQSVKLLSQQYRH
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
WKKPQLAAEMIEIVLWAARLHEVGIVINHKGMQKHSAYILQNIELPGFDKEQQRLLATLV
CCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHH
RYQINLFKLSELSKFARYDEQDVLALVRLLRLAIILNKSRQATEKTEQITLTFNRALTHW
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHEEEEEEHHHHHHH
TLKFAQGYLTHTPLICNELKAEKRVLNEMGLDITFN
HHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MKSENVMEKGTALSIPSRLEAREIAAIDLGSNSFHMIVARIMNGSIQILSRLKQKVQLAE
CCCCCHHHCCCEECCCCCCCHHHEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GLDENNVLNQAAITRGVNCLALFAERLQGFAPENVNVVGTYTLRRAVNNDEFLRQAKAVF
CCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHCC
PYPINIITGKTEAQTIYAGVSHTQPETGKKFVIDIGGGSTEMIIGENFTPILAESRHMGC
CCCEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCCCCCHH
VSFAKQFFPQGEISQLHFENARQAALMKIEDLAYEYRHLGWDVVLGSSGTIKTVHQVIMA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
NIDPNGLITPERLQQLIKQTLQHTHFEDLKLSGLIEERADVFVPGLAILSAVFETFGIQQ
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHH
MRYSDGALREGVMYSLEKTFQVSNIRERTALGLAEQFTIDQAQAERVHQSVKLLSQQYRH
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
WKKPQLAAEMIEIVLWAARLHEVGIVINHKGMQKHSAYILQNIELPGFDKEQQRLLATLV
CCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHH
RYQINLFKLSELSKFARYDEQDVLALVRLLRLAIILNKSRQATEKTEQITLTFNRALTHW
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHEEEEEEHHHHHHH
TLKFAQGYLTHTPLICNELKAEKRVLNEMGLDITFN
HHHHHCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]