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The map label for this gene is trmA

Identifier: 15603668

GI number: 15603668

Start: 2027039

End: 2028136

Strand: Direct

Name: trmA

Synonym: PM1803

Alternate gene names: 15603668

Gene position: 2027039-2028136 (Clockwise)

Preceding gene: 15603667

Following gene: 15603669

Centisome position: 89.79

GC content: 39.89

Gene sequence:

>1098_bases
ATGCAAGCGTTGCTTCCTATTGAACAATATGACAGCTTATTGGCGGAAAAACAGAAAAAACTGACCGCACTTTTAGCGCC
TTTTCATGCTCCCGCATTGGCGGTTTTTCCTTCACCTGTACAGCATTATCGTATGCGTGCAGAGTTTCGTATTTGGCATG
ACAAAGGGGATTTTTACCATATCATGTTCGATCAACGGACTCGTCAACGTTATCGTGTCGATTGTTTTCCGATGGCAAGT
CAGCTTATTAATCAGATGATGGATACGCTCTTACCGTTGTTAAAAACAAACAGAGTACTACATCATAAACTGTTCCAAAT
TGATTATTTAAGCACTTTAAGCAATAAGATCATTGTCAGTTTGCTATATCATAAAACCCTTGATGAAGAATGGGAACAGG
CAGCAAAACAATTAAAAGCAAGTTTGTTACAACAAGGTTTTGACTTACAACTGATTGGGCGTGCTAGCAAACAAAAAATC
TGTTTGGAGCAGGAGTTTGTCGATGAAGTGTTAACGGTGCATGGAAAATCTTACGTGTATCGTCAAGTGGAAAACAGCTT
TACTCAACCCAATGCGATCGTGAATCAGAAAATGCTTGAGTGGGCGGTGGACTGTACTCAAAACAGTGAAGGTGATTTGC
TTGAGCTTTATTGTGGCAACGGTAATTTTTCTATTGCGCTTGCACAAAATTTCCGCAAGGTTTTGGCGACAGAAATTGCT
AAACCCTCGGTGGCAGCCGCCCAATTTAATATTGCCGAAAATAGCATTAAGAATTTGCAAATTATTCGGATGTCCGCAGA
AGAATTCACGCAAGCGATGAATGGCGTGCGTGAATTTCATCGCTTAAAGGGCATTGATTTAAACGCCTATGAATGTAATA
CCATTTTTGTGGATCCGCCTCGTGCTGGTTTAGATGAGGAAACGGTGAAATTAGTACAGAATTATGATCGTATTTTGTAT
ATTTCATGTAATCCACATACTTTGTGCGATAATTTACAACATCTTTGTCAAACACACCGTATTGAAAAAGCTGCCTTGTT
CGATCAATTCCCCTATACCGAACATATGGAAGCCGGTGTGTGGTTAATTCGGAAATAA

Upstream 100 bases:

>100_bases
TCTCAGCAGAATAATCTCGCGTCATCTTATAAAAGGCTTGGACAATGAAACTGAGCCTTTTATCTTCATTTCATACAGAC
ATCATTATAGAACTCATCTT

Downstream 100 bases:

>100_bases
GCCATGAATATTCAACTGATTTGCGAAACGGAAAATCCTGAAAAATTCACCGCACTTTGTACACAATATGGTTTAGTGCA
CGATCCGGAAAGTTATTTGG

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MQALLPIEQYDSLLAEKQKKLTALLAPFHAPALAVFPSPVQHYRMRAEFRIWHDKGDFYHIMFDQRTRQRYRVDCFPMAS
QLINQMMDTLLPLLKTNRVLHHKLFQIDYLSTLSNKIIVSLLYHKTLDEEWEQAAKQLKASLLQQGFDLQLIGRASKQKI
CLEQEFVDEVLTVHGKSYVYRQVENSFTQPNAIVNQKMLEWAVDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIA
KPSVAAAQFNIAENSIKNLQIIRMSAEEFTQAMNGVREFHRLKGIDLNAYECNTIFVDPPRAGLDEETVKLVQNYDRILY
ISCNPHTLCDNLQHLCQTHRIEKAALFDQFPYTEHMEAGVWLIRK

Sequences:

>Translated_365_residues
MQALLPIEQYDSLLAEKQKKLTALLAPFHAPALAVFPSPVQHYRMRAEFRIWHDKGDFYHIMFDQRTRQRYRVDCFPMAS
QLINQMMDTLLPLLKTNRVLHHKLFQIDYLSTLSNKIIVSLLYHKTLDEEWEQAAKQLKASLLQQGFDLQLIGRASKQKI
CLEQEFVDEVLTVHGKSYVYRQVENSFTQPNAIVNQKMLEWAVDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIA
KPSVAAAQFNIAENSIKNLQIIRMSAEEFTQAMNGVREFHRLKGIDLNAYECNTIFVDPPRAGLDEETVKLVQNYDRILY
ISCNPHTLCDNLQHLCQTHRIEKAALFDQFPYTEHMEAGVWLIRK
>Mature_365_residues
MQALLPIEQYDSLLAEKQKKLTALLAPFHAPALAVFPSPVQHYRMRAEFRIWHDKGDFYHIMFDQRTRQRYRVDCFPMAS
QLINQMMDTLLPLLKTNRVLHHKLFQIDYLSTLSNKIIVSLLYHKTLDEEWEQAAKQLKASLLQQGFDLQLIGRASKQKI
CLEQEFVDEVLTVHGKSYVYRQVENSFTQPNAIVNQKMLEWAVDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIA
KPSVAAAQFNIAENSIKNLQIIRMSAEEFTQAMNGVREFHRLKGIDLNAYECNTIFVDPPRAGLDEETVKLVQNYDRILY
ISCNPHTLCDNLQHLCQTHRIEKAALFDQFPYTEHMEAGVWLIRK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=215, Percent_Identity=26.9767441860465, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI51173878, Length=215, Percent_Identity=26.9767441860465, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1790403, Length=361, Percent_Identity=66.7590027700831, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1789148, Length=183, Percent_Identity=31.6939890710383, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787083, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24666579, Length=438, Percent_Identity=23.0593607305936, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_PASMU (Q9CK32)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246742.1
- ProteinModelPortal:   Q9CK32
- SMR:   Q9CK32
- GeneID:   1245150
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1803
- NMPDR:   fig|272843.1.peg.1804
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- BioCyc:   PMUL272843:PM1803-MONOMER
- BRENDA:   2.1.1.35
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42268; Mature: 42268

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 323-323 BINDING 189-189 BINDING 217-217 BINDING 222-222 BINDING 238-238 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALLPIEQYDSLLAEKQKKLTALLAPFHAPALAVFPSPVQHYRMRAEFRIWHDKGDFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEEEEEECCCCEEE
IMFDQRTRQRYRVDCFPMASQLINQMMDTLLPLLKTNRVLHHKLFQIDYLSTLSNKIIVS
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LLYHKTLDEEWEQAAKQLKASLLQQGFDLQLIGRASKQKICLEQEFVDEVLTVHGKSYVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
RQVENSFTQPNAIVNQKMLEWAVDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIA
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHC
KPSVAAAQFNIAENSIKNLQIIRMSAEEFTQAMNGVREFHRLKGIDLNAYECNTIFVDPP
CCCHHHHEEEHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCC
RAGLDEETVKLVQNYDRILYISCNPHTLCDNLQHLCQTHRIEKAALFDQFPYTEHMEAGV
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCE
WLIRK
EEEEC
>Mature Secondary Structure
MQALLPIEQYDSLLAEKQKKLTALLAPFHAPALAVFPSPVQHYRMRAEFRIWHDKGDFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHEEEEEEECCCCEEE
IMFDQRTRQRYRVDCFPMASQLINQMMDTLLPLLKTNRVLHHKLFQIDYLSTLSNKIIVS
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
LLYHKTLDEEWEQAAKQLKASLLQQGFDLQLIGRASKQKICLEQEFVDEVLTVHGKSYVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHH
RQVENSFTQPNAIVNQKMLEWAVDCTQNSEGDLLELYCGNGNFSIALAQNFRKVLATEIA
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHC
KPSVAAAQFNIAENSIKNLQIIRMSAEEFTQAMNGVREFHRLKGIDLNAYECNTIFVDPP
CCCHHHHEEEHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCC
RAGLDEETVKLVQNYDRILYISCNPHTLCDNLQHLCQTHRIEKAALFDQFPYTEHMEAGV
CCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCE
WLIRK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100