Click here to switch to the map view.

The map label for this gene is aarF

Identifier: 15603553

GI number: 15603553

Start: 1896912

End: 1898495

Strand: Direct

Name: aarF

Synonym: PM1688

Alternate gene names: 15603553

Gene position: 1896912-1898495 (Clockwise)

Preceding gene: 15603552

Following gene: 15603554

Centisome position: 84.03

GC content: 41.86

Gene sequence:

>1584_bases
ATGCAAATTAAAGATATTTCCCACTTATATAACATCATTAAAACTTTCTTACTTTACGGGATTGATGAGGCCCTACCCCA
GCACCGCTATACACGTGCAATCCGCTGCTGGCGAAAAACCCTATTTTGGCTACGAAATCAACATAAGGACAAAACATTTG
GTTTGCGTTTACGCCTTGCGCTACAAGAGCTGGGACCAGTTTGGATCAAATTAGGACAAATGCTTTCCACGCGTCGTGAT
TTATTCCCCCCTGACATTGCTGATGAATTAGCGTTGTTACAAGATCAAGTCGATCCCTTTGATGGCAAGATCGCCAGAGC
ACAAATTGAAAAAGCGCTAGGCGCGCCACTCGAAACATGGTTTGATGAATTTAACGAAACTGCCTTAGCTTCAGCGTCGA
TTGCACAAGTCCATACGGCAAAATTTAAGCAAAACGCACCGCACTTAGAAAACCGTTTGGCAGGAAAAGAAGTGGTATTA
AAGGTTTTACGCCCTAACATCCAACAAATGATTAATGCTGACTTAAGTTTAATGTACAAAGTGGCAAGTTGGATCCCGCG
CATAAAAGCAGAAGGACGCAGACTACGTCCTGTGGAAGTCGTGCGCGAATATGAAAAAAACCTACGCGATGAATTAGACT
TACGCCGTGAAATGGCGAATGCGATTCAATTACGTGCCAATTTTGAAAACAGCCCAATGTTATACATTCCCGAAATGTAT
AAACAATTTTGTCATCAAACCGTCATCGTCATGGAACGTATTTATGGGATACCGGTTTCCAATATTGAAGAACTCCATGC
TAATGGCACCAATATGAAATTACTGGCGGAGCGTGGTGTACAGGTATTTTTTACTCAGGTTTTTCGTGATAGCTTTTTCC
ATGCAGACATGCACCCGGGTAATATTTTTGTCAATCGAGCACATCCTGACGATCCCCAATATATTGGGATCGATTGTGGT
ATTGTGGGACGCCTCAATGATCATGATAAACGTTATTTAGCGGAAAGTTTTGTGGCGTTTTTTAATCGCGACTATCGCCG
TGTGGCGGAAATGCATGTTGCATCGGGTTGGACCCCCAAAGACACGAATATTGATGATTTTGAGCAAGCTTTTCGTGAGG
TGTGTGAGCCAATTTTTGCTAAACCCTTATCCGAGATTTCCTTTGGACATGTGCTCTTAAACCTGTTTAATGTGGCACGC
GAATACAATATGGAAGTCCAGCCACAATTGGTGTTACTACAAAAAACGTTACTTTACATTGAAGGGTTAGGCAGACAGCT
TTATCCACAACTAGATTTATGGGATACCGCAAAGCCCTTCTTACAAAAATGGCTCGATGAGCAAATGGGCATAAAAGCCT
TTACTAAATCTGTTAAACAAAAACTGCCATATTGGCGTGAACATTTGGTTGATTTACCAGAAAATGTGATGGATGCCCTC
GCGCAACAAAAAATTATCGCTGACGAGTTAATCCATTTAAATCGCACCTTAGCCAAAAAACGTAACATTCCTCACTTCAC
CTCCTTTATTCTGGGGTTATGCACTGGCTTAGCGATCTGGCTTTTAATTTACTTACTAAGCTGA

Upstream 100 bases:

>100_bases
ATTTTTGTGATCAAGTTAAACACTTAGAACACCAAACCGCGCATTTAGAACAAAAAATTGAAATGCTTTTCAAAAAGAAA
TAGCCCAATAGGGACAACCC

Downstream 100 bases:

>100_bases
TCAAACGCAAAAGAAGAGAAAATAATGTGAATTGGCTATTGTTCAAACGCAGTAAAAAAGCTATATTCACCAACATTCAT
TTTTCAACATACTAAAGAAG

Product: hypothetical protein

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MQIKDISHLYNIIKTFLLYGIDEALPQHRYTRAIRCWRKTLFWLRNQHKDKTFGLRLRLALQELGPVWIKLGQMLSTRRD
LFPPDIADELALLQDQVDPFDGKIARAQIEKALGAPLETWFDEFNETALASASIAQVHTAKFKQNAPHLENRLAGKEVVL
KVLRPNIQQMINADLSLMYKVASWIPRIKAEGRRLRPVEVVREYEKNLRDELDLRREMANAIQLRANFENSPMLYIPEMY
KQFCHQTVIVMERIYGIPVSNIEELHANGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVNRAHPDDPQYIGIDCG
IVGRLNDHDKRYLAESFVAFFNRDYRRVAEMHVASGWTPKDTNIDDFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAR
EYNMEVQPQLVLLQKTLLYIEGLGRQLYPQLDLWDTAKPFLQKWLDEQMGIKAFTKSVKQKLPYWREHLVDLPENVMDAL
AQQKIIADELIHLNRTLAKKRNIPHFTSFILGLCTGLAIWLLIYLLS

Sequences:

>Translated_527_residues
MQIKDISHLYNIIKTFLLYGIDEALPQHRYTRAIRCWRKTLFWLRNQHKDKTFGLRLRLALQELGPVWIKLGQMLSTRRD
LFPPDIADELALLQDQVDPFDGKIARAQIEKALGAPLETWFDEFNETALASASIAQVHTAKFKQNAPHLENRLAGKEVVL
KVLRPNIQQMINADLSLMYKVASWIPRIKAEGRRLRPVEVVREYEKNLRDELDLRREMANAIQLRANFENSPMLYIPEMY
KQFCHQTVIVMERIYGIPVSNIEELHANGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVNRAHPDDPQYIGIDCG
IVGRLNDHDKRYLAESFVAFFNRDYRRVAEMHVASGWTPKDTNIDDFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAR
EYNMEVQPQLVLLQKTLLYIEGLGRQLYPQLDLWDTAKPFLQKWLDEQMGIKAFTKSVKQKLPYWREHLVDLPENVMDAL
AQQKIIADELIHLNRTLAKKRNIPHFTSFILGLCTGLAIWLLIYLLS
>Mature_527_residues
MQIKDISHLYNIIKTFLLYGIDEALPQHRYTRAIRCWRKTLFWLRNQHKDKTFGLRLRLALQELGPVWIKLGQMLSTRRD
LFPPDIADELALLQDQVDPFDGKIARAQIEKALGAPLETWFDEFNETALASASIAQVHTAKFKQNAPHLENRLAGKEVVL
KVLRPNIQQMINADLSLMYKVASWIPRIKAEGRRLRPVEVVREYEKNLRDELDLRREMANAIQLRANFENSPMLYIPEMY
KQFCHQTVIVMERIYGIPVSNIEELHANGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVNRAHPDDPQYIGIDCG
IVGRLNDHDKRYLAESFVAFFNRDYRRVAEMHVASGWTPKDTNIDDFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAR
EYNMEVQPQLVLLQKTLLYIEGLGRQLYPQLDLWDTAKPFLQKWLDEQMGIKAFTKSVKQKLPYWREHLVDLPENVMDAL
AQQKIIADELIHLNRTLAKKRNIPHFTSFILGLCTGLAIWLLIYLLS

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily

Homologues:

Organism=Homo sapiens, GI32261307, Length=338, Percent_Identity=25.7396449704142, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI41393593, Length=282, Percent_Identity=24.468085106383, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI40254938, Length=286, Percent_Identity=25.1748251748252, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI34147522, Length=198, Percent_Identity=29.7979797979798, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI217035081, Length=240, Percent_Identity=24.5833333333333, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI2367309, Length=520, Percent_Identity=64.6153846153846, Blast_Score=700, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559152, Length=284, Percent_Identity=26.056338028169, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565180, Length=257, Percent_Identity=26.4591439688716, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325148, Length=440, Percent_Identity=26.5909090909091, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323282, Length=255, Percent_Identity=25.4901960784314, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321319, Length=254, Percent_Identity=20.8661417322835, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI22024280, Length=261, Percent_Identity=27.9693486590038, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24662575, Length=245, Percent_Identity=26.1224489795918, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIB_PASMU (Q9CKD4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246627.1
- ProteinModelPortal:   Q9CKD4
- GeneID:   1245035
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1688
- NMPDR:   fig|272843.1.peg.1689
- HOGENOM:   HBG593509
- OMA:   RIQRVVI
- ProtClustDB:   CLSK870574
- BioCyc:   PMUL272843:PM1688-MONOMER
- HAMAP:   MF_00414
- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232
- TIGRFAMs:   TIGR01982

Pfam domain/function: PF03109 ABC1; SSF56112 Kinase_like

EC number: NA

Molecular weight: Translated: 61409; Mature: 61409

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe82fc38)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKDISHLYNIIKTFLLYGIDEALPQHRYTRAIRCWRKTLFWLRNQHKDKTFGLRLRLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQELGPVWIKLGQMLSTRRDLFPPDIADELALLQDQVDPFDGKIARAQIEKALGAPLETW
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
FDEFNETALASASIAQVHTAKFKQNAPHLENRLAGKEVVLKVLRPNIQQMINADLSLMYK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASWIPRIKAEGRRLRPVEVVREYEKNLRDELDLRREMANAIQLRANFENSPMLYIPEMY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHH
KQFCHQTVIVMERIYGIPVSNIEELHANGTNMKLLAERGVQVFFTQVFRDSFFHADMHPG
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCC
NIFVNRAHPDDPQYIGIDCGIVGRLNDHDKRYLAESFVAFFNRDYRRVAEMHVASGWTPK
CEEEECCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTNIDDFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREYNMEVQPQLVLLQKTLLYI
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EGLGRQLYPQLDLWDTAKPFLQKWLDEQMGIKAFTKSVKQKLPYWREHLVDLPENVMDAL
HHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
AQQKIIADELIHLNRTLAKKRNIPHFTSFILGLCTGLAIWLLIYLLS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure
MQIKDISHLYNIIKTFLLYGIDEALPQHRYTRAIRCWRKTLFWLRNQHKDKTFGLRLRLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQELGPVWIKLGQMLSTRRDLFPPDIADELALLQDQVDPFDGKIARAQIEKALGAPLETW
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHH
FDEFNETALASASIAQVHTAKFKQNAPHLENRLAGKEVVLKVLRPNIQQMINADLSLMYK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASWIPRIKAEGRRLRPVEVVREYEKNLRDELDLRREMANAIQLRANFENSPMLYIPEMY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHH
KQFCHQTVIVMERIYGIPVSNIEELHANGTNMKLLAERGVQVFFTQVFRDSFFHADMHPG
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCC
NIFVNRAHPDDPQYIGIDCGIVGRLNDHDKRYLAESFVAFFNRDYRRVAEMHVASGWTPK
CEEEECCCCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DTNIDDFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREYNMEVQPQLVLLQKTLLYI
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EGLGRQLYPQLDLWDTAKPFLQKWLDEQMGIKAFTKSVKQKLPYWREHLVDLPENVMDAL
HHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
AQQKIIADELIHLNRTLAKKRNIPHFTSFILGLCTGLAIWLLIYLLS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100