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The map label for this gene is ispH

Identifier: 15603529

GI number: 15603529

Start: 1872710

End: 1873654

Strand: Direct

Name: ispH

Synonym: PM1664

Alternate gene names: 15603529

Gene position: 1872710-1873654 (Clockwise)

Preceding gene: 15603528

Following gene: 15603530

Centisome position: 82.96

GC content: 44.97

Gene sequence:

>945_bases
ATGAAAATTATTTTAGCCAATCCACGTGGTTTCTGTGCCGGTGTTGATCGTGCGATTAGTATCGTAGAATCTGCGTTAGA
GATTCACGGTTCACCTATTTATGTGCGTCATGAAGTGGTACACAACCGCTTTGTTGTTAATGGGTTACGCGAACGTGGGG
CAATTTTTGTAGAAGAATTACATGAAGTACCGGATGGTGCGATTGTGATTTTCTCAGCTCATGGTGTTTCACAAGCGGTC
CGCCAAGAAGCCAAAAATCGCAATCTCAAAGTGTTTGATGCGACCTGTCCTTTGGTGACAAAAGTCCATATGCAAGTGGC
AAGGGCAAGCCGTAAAGGCACCAAGGCGATCTTAATTGGTCATGAGGGGCATCCAGAAGTCGAAGGCACGATGGGGCAAT
ATGACAACCCTGAAGGGGGCATTTTCTTGGTGGAAAGTGTGGAAGATATTGCTAAGTTAGGGTTAAGACAAGAAGATGAT
TTAACCTTTATGACACAGACTACCTTGTCAATTGACGATACCAGTGAAGTGATTGAAGCACTCAAACAAAAATACCCGGC
TATTCAAGGTCCGCGTAAAAACGATATTTGTTATGCCACCACCAACCGCCAACAAGCCGTGCGTGAACTGGCACAACAAT
CTGATTTAGTCATTGTCGTTGGTTCTAAAAATTCGTCTAACTCTAACCGGTTAGCTGAGCTTGCCTCTCGTATGGGCGTC
GTCTCCAAACTGATTGATGATGCAAATGACATTGATCCAACTTGGCTAGAAAATGTAACTACCATTGGGATTACTGCCGG
TGCCTCTGCCCCAGAAGTGTTAGTCCAATCGGTCGTCAAACGTCTGCAAGAGTTAGGTGTTACCCGTGTAGAAGAGCTAC
AAGGGTGTGAAGAAAATACGGTGTTTGAAGTGCCGAGAGAACTGCGTATTACCGAAGTCAATTAG

Upstream 100 bases:

>100_bases
TGCCGACATCGCCATTTCCCTTGGTGCAGGATTGATGATTCTTGATGCGTTCAAAAATCGCCATGAACCTGAACAACGTA
CAGAATAATAGAGGACGATG

Downstream 100 bases:

>100_bases
CCTCCCTCATTGCTTAATTTATCCAGACATGATGAAAACCACGGATTGAATACCCGTGGTTTTTTTATTGGCTCAACAGC
ATGATTCAATCCTATCATCA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKIILANPRGFCAGVDRAISIVESALEIHGSPIYVRHEVVHNRFVVNGLRERGAIFVEELHEVPDGAIVIFSAHGVSQAV
RQEAKNRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHEGHPEVEGTMGQYDNPEGGIFLVESVEDIAKLGLRQEDD
LTFMTQTTLSIDDTSEVIEALKQKYPAIQGPRKNDICYATTNRQQAVRELAQQSDLVIVVGSKNSSNSNRLAELASRMGV
VSKLIDDANDIDPTWLENVTTIGITAGASAPEVLVQSVVKRLQELGVTRVEELQGCEENTVFEVPRELRITEVN

Sequences:

>Translated_314_residues
MKIILANPRGFCAGVDRAISIVESALEIHGSPIYVRHEVVHNRFVVNGLRERGAIFVEELHEVPDGAIVIFSAHGVSQAV
RQEAKNRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHEGHPEVEGTMGQYDNPEGGIFLVESVEDIAKLGLRQEDD
LTFMTQTTLSIDDTSEVIEALKQKYPAIQGPRKNDICYATTNRQQAVRELAQQSDLVIVVGSKNSSNSNRLAELASRMGV
VSKLIDDANDIDPTWLENVTTIGITAGASAPEVLVQSVVKRLQELGVTRVEELQGCEENTVFEVPRELRITEVN
>Mature_314_residues
MKIILANPRGFCAGVDRAISIVESALEIHGSPIYVRHEVVHNRFVVNGLRERGAIFVEELHEVPDGAIVIFSAHGVSQAV
RQEAKNRNLKVFDATCPLVTKVHMQVARASRKGTKAILIGHEGHPEVEGTMGQYDNPEGGIFLVESVEDIAKLGLRQEDD
LTFMTQTTLSIDDTSEVIEALKQKYPAIQGPRKNDICYATTNRQQAVRELAQQSDLVIVVGSKNSSNSNRLAELASRMGV
VSKLIDDANDIDPTWLENVTTIGITAGASAPEVLVQSVVKRLQELGVTRVEELQGCEENTVFEVPRELRITEVN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=71.9354838709677, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_PASMU (P57960)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246603.1
- PRIDE:   P57960
- GeneID:   1245011
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1664
- NMPDR:   fig|272843.1.peg.1665
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   PMUL272843:PM1664-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34470; Mature: 34470

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIILANPRGFCAGVDRAISIVESALEIHGSPIYVRHEVVHNRFVVNGLRERGAIFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDGAIVIFSAHGVSQAVRQEAKNRNLKVFDATCPLVTKVHMQVARASRKGTKAILIG
HHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HEGHPEVEGTMGQYDNPEGGIFLVESVEDIAKLGLRQEDDLTFMTQTTLSIDDTSEVIEA
CCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHH
LKQKYPAIQGPRKNDICYATTNRQQAVRELAQQSDLVIVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
VSKLIDDANDIDPTWLENVTTIGITAGASAPEVLVQSVVKRLQELGVTRVEELQGCEENT
HHHHHHCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
VFEVPRELRITEVN
HHHCCCCCEEEECC
>Mature Secondary Structure
MKIILANPRGFCAGVDRAISIVESALEIHGSPIYVRHEVVHNRFVVNGLRERGAIFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHH
HEVPDGAIVIFSAHGVSQAVRQEAKNRNLKVFDATCPLVTKVHMQVARASRKGTKAILIG
HHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEE
HEGHPEVEGTMGQYDNPEGGIFLVESVEDIAKLGLRQEDDLTFMTQTTLSIDDTSEVIEA
CCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHH
LKQKYPAIQGPRKNDICYATTNRQQAVRELAQQSDLVIVVGSKNSSNSNRLAELASRMGV
HHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
VSKLIDDANDIDPTWLENVTTIGITAGASAPEVLVQSVVKRLQELGVTRVEELQGCEENT
HHHHHHCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
VFEVPRELRITEVN
HHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100