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The map label for this gene is yiaO [H]

Identifier: 15603516

GI number: 15603516

Start: 1859742

End: 1860746

Strand: Direct

Name: yiaO [H]

Synonym: PM1651

Alternate gene names: 15603516

Gene position: 1859742-1860746 (Clockwise)

Preceding gene: 15603502

Following gene: 15603517

Centisome position: 82.38

GC content: 36.72

Gene sequence:

>1005_bases
ATGGAGACTTTCACTATGAGTACATCATTATTAAAAAGATTAACCATTGTGGCATCTTTATCACTCTTATTTGCGAATAG
TATTCTGGCTAAAGATCTCGTTGTATCCACAACGGCTAGTCTAGGCTCTTTACAATATGACACGACAAAATATTTTGTGG
ATGTCACAAACCAAAAACTTTCGGAAGCTAAATTAGACTACCGTTTAAATTTCTTTGGTTCTGGTCAACTAGGGAATGAT
AAAGACACGCAGCAAAAATTAAAATTAGGGACGATTGATATAGCCCTTCTCTCTTCAACACTCGCTACGAACATTCCTCA
AATGGCGATTTTTGAATTACCTTTCTTAATCACAAACCGTCAACAGCTGAATAAAATTGAGCAACAAGTCTTCTATCCTT
TCGTTGCACCAGAAGTAGAGAAAAAAGGATATAAAATCATTGGGTTATGGGAGAATGGATTTAGAAGTATTACTAACAAT
ACACGCCCAATCAATCGCCCTGAAGACTTGAAAGGATTAAAAATTCGTACCCCAAGTAGCTCTTGGCGTTTAAAAATGTT
TAAAGCATGGGGTGCTAACCCAACACCAATTCCTTTCGGAGACGTGTTTATTGGTTTAAGAACCGGCGTCATAGACGGTC
AAGAAAACCCACTAACAAACATCTATGCAGCAAAACTCCAAGAGGTTCAAAAATATCTCAGTATCACTAACCATGTTTAT
TCTCCAGCTTACCTTACGGTAGGCAAAAACACATATCAAAAATTACCTGAAAATGTTCGGAAAATTATTGAAGCTGGGGC
AAAAGAAGCACAAACATGGGGATATCAGGAAGCCGAAAAACGAGAAAGTGAACTGGAAAAGAAATTAGTAGAAAGTGGCA
TGACACTTAATAATGCAAATATTCAAGCATTTATCGAAGCGAGCCAACCTATCTACGATGAATTCATTTCTGAAGTTCCA
AATGGAAAAGAATTATTAGAGAAAATGAAGGATACGCTTAAGTAA

Upstream 100 bases:

>100_bases
CACCTTTGGTTATATTGAACAATATTCGCATATCCCAATCACTTTTCAACACTGAGGATTTATTAGGCTGTTTTATCCTT
CCACAAACATAAAACTACTA

Downstream 100 bases:

>100_bases
AAAGAAACCGGGGGTAAGTTTTTATGGACGCACTCTTTAACCTAACGAAAAGAATTTTAACGCTGATTTCAGTACTTATT
TTAAGTGCATTGGCCATTAT

Product: hypothetical protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
METFTMSTSLLKRLTIVASLSLLFANSILAKDLVVSTTASLGSLQYDTTKYFVDVTNQKLSEAKLDYRLNFFGSGQLGND
KDTQQKLKLGTIDIALLSSTLATNIPQMAIFELPFLITNRQQLNKIEQQVFYPFVAPEVEKKGYKIIGLWENGFRSITNN
TRPINRPEDLKGLKIRTPSSSWRLKMFKAWGANPTPIPFGDVFIGLRTGVIDGQENPLTNIYAAKLQEVQKYLSITNHVY
SPAYLTVGKNTYQKLPENVRKIIEAGAKEAQTWGYQEAEKRESELEKKLVESGMTLNNANIQAFIEASQPIYDEFISEVP
NGKELLEKMKDTLK

Sequences:

>Translated_334_residues
METFTMSTSLLKRLTIVASLSLLFANSILAKDLVVSTTASLGSLQYDTTKYFVDVTNQKLSEAKLDYRLNFFGSGQLGND
KDTQQKLKLGTIDIALLSSTLATNIPQMAIFELPFLITNRQQLNKIEQQVFYPFVAPEVEKKGYKIIGLWENGFRSITNN
TRPINRPEDLKGLKIRTPSSSWRLKMFKAWGANPTPIPFGDVFIGLRTGVIDGQENPLTNIYAAKLQEVQKYLSITNHVY
SPAYLTVGKNTYQKLPENVRKIIEAGAKEAQTWGYQEAEKRESELEKKLVESGMTLNNANIQAFIEASQPIYDEFISEVP
NGKELLEKMKDTLK
>Mature_334_residues
METFTMSTSLLKRLTIVASLSLLFANSILAKDLVVSTTASLGSLQYDTTKYFVDVTNQKLSEAKLDYRLNFFGSGQLGND
KDTQQKLKLGTIDIALLSSTLATNIPQMAIFELPFLITNRQQLNKIEQQVFYPFVAPEVEKKGYKIIGLWENGFRSITNN
TRPINRPEDLKGLKIRTPSSSWRLKMFKAWGANPTPIPFGDVFIGLRTGVIDGQENPLTNIYAAKLQEVQKYLSITNHVY
SPAYLTVGKNTYQKLPENVRKIIEAGAKEAQTWGYQEAEKRESELEKKLVESGMTLNNANIQAFIEASQPIYDEFISEVP
NGKELLEKMKDTLK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=320, Percent_Identity=30.9375, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37651; Mature: 37651

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFTMSTSLLKRLTIVASLSLLFANSILAKDLVVSTTASLGSLQYDTTKYFVDVTNQKL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEECCCHH
SEAKLDYRLNFFGSGQLGNDKDTQQKLKLGTIDIALLSSTLATNIPQMAIFELPFLITNR
HHHHHCEEEEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCHHCCCH
QQLNKIEQQVFYPFVAPEVEKKGYKIIGLWENGFRSITNNTRPINRPEDLKGLKIRTPSS
HHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEECCCC
SWRLKMFKAWGANPTPIPFGDVFIGLRTGVIDGQENPLTNIYAAKLQEVQKYLSITNHVY
CCEEEEEEECCCCCCCCCHHHHHEEHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SPAYLTVGKNTYQKLPENVRKIIEAGAKEAQTWGYQEAEKRESELEKKLVESGMTLNNAN
CCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCCC
IQAFIEASQPIYDEFISEVPNGKELLEKMKDTLK
EEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
METFTMSTSLLKRLTIVASLSLLFANSILAKDLVVSTTASLGSLQYDTTKYFVDVTNQKL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEECCCHH
SEAKLDYRLNFFGSGQLGNDKDTQQKLKLGTIDIALLSSTLATNIPQMAIFELPFLITNR
HHHHHCEEEEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHCCHHCCCH
QQLNKIEQQVFYPFVAPEVEKKGYKIIGLWENGFRSITNNTRPINRPEDLKGLKIRTPSS
HHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCEEECCCC
SWRLKMFKAWGANPTPIPFGDVFIGLRTGVIDGQENPLTNIYAAKLQEVQKYLSITNHVY
CCEEEEEEECCCCCCCCCHHHHHEEHHHCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SPAYLTVGKNTYQKLPENVRKIIEAGAKEAQTWGYQEAEKRESELEKKLVESGMTLNNAN
CCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCEECCCC
IQAFIEASQPIYDEFISEVPNGKELLEKMKDTLK
EEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]