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The map label for this gene is murB

Identifier: 15603454

GI number: 15603454

Start: 1799981

End: 1801006

Strand: Reverse

Name: murB

Synonym: PM1589

Alternate gene names: 15603454

Gene position: 1801006-1799981 (Counterclockwise)

Preceding gene: 15603455

Following gene: 15603453

Centisome position: 79.78

GC content: 42.98

Gene sequence:

>1026_bases
ATGCAAAATCTCCAACCCTTTCATACTTTTTCTCTGCCCGTACAAGCACAGAAAATTATTGAAATTACCGATATTGAGCA
ACTGAAACAACAATGGGCTGCATGTCAGACCAATAAACTACCGGTGTTATTGTTAGGTCAAGGCAGTAATGTCCTTTTCT
TACGCGATTTTCAAGGTGTTGTCTTGCTTAATCGCTTAATGGGCATTCAACATACACAAGATGCTGATTTTCATTATTTA
CATGTCAATGGAGGAGAAAATTGGCATCAATTGGTCGCGTGGTCAATACAGCAAGGCATCTTTGGACTCGAAAATCTCGC
ACTGATTCCGGGTTGTGCAGGTTCAGCACCAATCCAAAACATCGGGGCTTATGGTGTGGAATTTAAAGATGTGTGTGACT
ATGTGGAAGTACTCAACTTACACACGAATGAAATCTTTCGCCTTAGCAATGAACAATGCCAATTTGGTTATCGCGACAGC
ATTTTTAAACACGCCTATGCCACGGGCTATGTAATCATTGCCGTAGGTTTGAAACTCAGCAAAAACTGGATACCCGTTTT
AAAATATGGGTCACTGGCTAATTTGGAGAAAAGTGCGGTCAATGCCCAGCAAATTTTTGACGAAGTGTGTGCGATACGAC
GCAGTAAATTACCTGACCCAGCAGAATTCGGCAATGCCGGTAGTTTCTTCAAAAATCCGGTCATTAGTAAAGTGGATTTT
GATCACTTACAGCAAGAATATCCTGACATACCGCATTTTCCGCAAGCTGACGGACGTGTCAAATTAGCCGCAGGTTGGCT
CATTGATCAATGTGGCTTGAAAGGGTATCAACTGGGTGGTGCAGCAGTCCACCAGCAACAAGCGCTGGTTCTGATCAACC
AACAGGATGCAACGAGTAGTGATGTGGTAGAGTTAGCACATCACATTCGCCAAAAAGTTGCTCAACGCTTTGCTGTCTGG
TTACAACCAGAAGTTCGCTTTATTGACGAAGACGGTGAAGTGGATAGTGAACAAGCCATTTGTTAA

Upstream 100 bases:

>100_bases
TTATTCTACGACATTGCCGTTACAACCCATTGACAATCACCAAGCAATATTGGTCAATAACGAAACAAAAATCATCAGAG
GAAACCATAAAGGATAACCT

Downstream 100 bases:

>100_bases
AGGAGCCATCAGATGAATTTCAATACGCTATTAAATACCTTGCCACCAATTGAAGACTTAGTTGGGCTTGCTATCATGGA
CGGTGAAACACAAATCCATT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQNLQPFHTFSLPVQAQKIIEITDIEQLKQQWAACQTNKLPVLLLGQGSNVLFLRDFQGVVLLNRLMGIQHTQDADFHYL
HVNGGENWHQLVAWSIQQGIFGLENLALIPGCAGSAPIQNIGAYGVEFKDVCDYVEVLNLHTNEIFRLSNEQCQFGYRDS
IFKHAYATGYVIIAVGLKLSKNWIPVLKYGSLANLEKSAVNAQQIFDEVCAIRRSKLPDPAEFGNAGSFFKNPVISKVDF
DHLQQEYPDIPHFPQADGRVKLAAGWLIDQCGLKGYQLGGAAVHQQQALVLINQQDATSSDVVELAHHIRQKVAQRFAVW
LQPEVRFIDEDGEVDSEQAIC

Sequences:

>Translated_341_residues
MQNLQPFHTFSLPVQAQKIIEITDIEQLKQQWAACQTNKLPVLLLGQGSNVLFLRDFQGVVLLNRLMGIQHTQDADFHYL
HVNGGENWHQLVAWSIQQGIFGLENLALIPGCAGSAPIQNIGAYGVEFKDVCDYVEVLNLHTNEIFRLSNEQCQFGYRDS
IFKHAYATGYVIIAVGLKLSKNWIPVLKYGSLANLEKSAVNAQQIFDEVCAIRRSKLPDPAEFGNAGSFFKNPVISKVDF
DHLQQEYPDIPHFPQADGRVKLAAGWLIDQCGLKGYQLGGAAVHQQQALVLINQQDATSSDVVELAHHIRQKVAQRFAVW
LQPEVRFIDEDGEVDSEQAIC
>Mature_341_residues
MQNLQPFHTFSLPVQAQKIIEITDIEQLKQQWAACQTNKLPVLLLGQGSNVLFLRDFQGVVLLNRLMGIQHTQDADFHYL
HVNGGENWHQLVAWSIQQGIFGLENLALIPGCAGSAPIQNIGAYGVEFKDVCDYVEVLNLHTNEIFRLSNEQCQFGYRDS
IFKHAYATGYVIIAVGLKLSKNWIPVLKYGSLANLEKSAVNAQQIFDEVCAIRRSKLPDPAEFGNAGSFFKNPVISKVDF
DHLQQEYPDIPHFPQADGRVKLAAGWLIDQCGLKGYQLGGAAVHQQQALVLINQQDATSSDVVELAHHIRQKVAQRFAVW
LQPEVRFIDEDGEVDSEQAIC

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=338, Percent_Identity=56.8047337278106, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_PASMU (P57952)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246528.1
- ProteinModelPortal:   P57952
- SMR:   P57952
- GeneID:   1244936
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1589
- NMPDR:   fig|272843.1.peg.1590
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK00046
- BioCyc:   PMUL272843:PM1589-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 38163; Mature: 38163

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 158-158 ACT_SITE 228-228 ACT_SITE 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLQPFHTFSLPVQAQKIIEITDIEQLKQQWAACQTNKLPVLLLGQGSNVLFLRDFQGV
CCCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHH
VLLNRLMGIQHTQDADFHYLHVNGGENWHQLVAWSIQQGIFGLENLALIPGCAGSAPIQN
HHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHH
IGAYGVEFKDVCDYVEVLNLHTNEIFRLSNEQCQFGYRDSIFKHAYATGYVIIAVGLKLS
CCCCCCCHHHHHHHHHHHHCCCHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEC
KNWIPVLKYGSLANLEKSAVNAQQIFDEVCAIRRSKLPDPAEFGNAGSFFKNPVISKVDF
CCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCCCCCCCCH
DHLQQEYPDIPHFPQADGRVKLAAGWLIDQCGLKGYQLGGAAVHQQQALVLINQQDATSS
HHHHHHCCCCCCCCCCCCEEEEEECHHHHHCCCCCEECCCHHHHCCCEEEEEECCCCCHH
DVVELAHHIRQKVAQRFAVWLQPEVRFIDEDGEVDSEQAIC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQNLQPFHTFSLPVQAQKIIEITDIEQLKQQWAACQTNKLPVLLLGQGSNVLFLRDFQGV
CCCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCHH
VLLNRLMGIQHTQDADFHYLHVNGGENWHQLVAWSIQQGIFGLENLALIPGCAGSAPIQN
HHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHH
IGAYGVEFKDVCDYVEVLNLHTNEIFRLSNEQCQFGYRDSIFKHAYATGYVIIAVGLKLS
CCCCCCCHHHHHHHHHHHHCCCHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEEEEEEEC
KNWIPVLKYGSLANLEKSAVNAQQIFDEVCAIRRSKLPDPAEFGNAGSFFKNPVISKVDF
CCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCCCCCCCCH
DHLQQEYPDIPHFPQADGRVKLAAGWLIDQCGLKGYQLGGAAVHQQQALVLINQQDATSS
HHHHHHCCCCCCCCCCCCEEEEEECHHHHHCCCCCEECCCHHHHCCCEEEEEECCCCCHH
DVVELAHHIRQKVAQRFAVWLQPEVRFIDEDGEVDSEQAIC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100