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The map label for this gene is rpoH [H]

Identifier: 15603449

GI number: 15603449

Start: 1794303

End: 1795148

Strand: Reverse

Name: rpoH [H]

Synonym: PM1584

Alternate gene names: 15603449

Gene position: 1795148-1794303 (Counterclockwise)

Preceding gene: 15603450

Following gene: 15603448

Centisome position: 79.52

GC content: 41.49

Gene sequence:

>846_bases
ATGAACAAAGACACACAAACCATGATGTTGGTTCCACAAGGCAGTATCGAAGCTTATATTCGTGCGGCAAATGAATATCC
TATGCTTACCGCTGAGGAAGAAAAGGAATTGGCGGAGCGTTTGTATTATCAAGAAGATTTGGAAGCAGCGAAAAAACTGA
TCCTTTCTCATCTTCGCTTTGTTATTCATGTCGCACGTGGCTATTCAGGCTATGGTCTTCCACAAGCCGATTTGATCCAA
GAAGGCAATATTGGCTTAATGAAGGCGGTGAAACGTTTTAACCCAGAAGTAGGGGTACGTTTAGTGTCCTTTGCGGTGCA
TTGGATCAAAGCAGAAATTCATGAATACGTTCTGCGCAACTGGCGTATTGTGAAAGTAGCAACGACCAAAGCACAACGTA
AATTATTCTTTAATCTACGCAAAACAAAGCAACGTTTAGGCTGGTTTAACGAAAACGAAGTAGAAATGGTTGCTAATGAA
CTTGGTGTTTCGCCAGCAGATGTGATGGAAATGGAGTCCCGTATGACGGGTGCCGATGTGGCATTTGATTTGCCAACAGA
TGACAACGAAGAAACCTATTCACCATCTCTATATTTAGAAGACAAAGGTTCCAACTTCGCGGCGGAACTTGAAAATGAAA
ATTATGAAGCTCAAGCCACAGAACAATTAAGTGTGGCACTTGAGGGACTGGATGAACGTAGTCTTGATATTATTAAAGCG
CGTTGGTTAGATGAAAACAAAGCTACTTTGCAAGATTTAGCCGCCAAATACAATATTTCTGCTGAGCGTGTACGCCAATT
AGAAACGAATGCACTGAAAAAACTCAAAAGTGCGGTCAATTTCTAA

Upstream 100 bases:

>100_bases
AGTTCTGCCAACAGGAAAGCTCCATAATCATTGGCGCCAGTTGGATTTTTATGCTATAACTAGTTTGAGATGTTATCTTC
ATTTTTTAAGAAGGAAAGCA

Downstream 100 bases:

>100_bases
TCCTGTTTTATCAAGTAAAAGCCAAAGTGATCGACTTTGGCTTTTTTGTTTTCATACAAATAGATTATAAAATATCAGCA
AGAATGGTATAGTAATCCCT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNKDTQTMMLVPQGSIEAYIRAANEYPMLTAEEEKELAERLYYQEDLEAAKKLILSHLRFVIHVARGYSGYGLPQADLIQ
EGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVEMVANE
LGVSPADVMEMESRMTGADVAFDLPTDDNEETYSPSLYLEDKGSNFAAELENENYEAQATEQLSVALEGLDERSLDIIKA
RWLDENKATLQDLAAKYNISAERVRQLETNALKKLKSAVNF

Sequences:

>Translated_281_residues
MNKDTQTMMLVPQGSIEAYIRAANEYPMLTAEEEKELAERLYYQEDLEAAKKLILSHLRFVIHVARGYSGYGLPQADLIQ
EGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVEMVANE
LGVSPADVMEMESRMTGADVAFDLPTDDNEETYSPSLYLEDKGSNFAAELENENYEAQATEQLSVALEGLDERSLDIIKA
RWLDENKATLQDLAAKYNISAERVRQLETNALKKLKSAVNF
>Mature_281_residues
MNKDTQTMMLVPQGSIEAYIRAANEYPMLTAEEEKELAERLYYQEDLEAAKKLILSHLRFVIHVARGYSGYGLPQADLIQ
EGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVEMVANE
LGVSPADVMEMESRMTGADVAFDLPTDDNEETYSPSLYLEDKGSNFAAELENENYEAQATEQLSVALEGLDERSLDIIKA
RWLDENKATLQDLAAKYNISAERVRQLETNALKKLKSAVNF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=282, Percent_Identity=70.5673758865248, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1789098, Length=278, Percent_Identity=27.3381294964029, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=28.6821705426357, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32115; Mature: 32115

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDTQTMMLVPQGSIEAYIRAANEYPMLTAEEEKELAERLYYQEDLEAAKKLILSHLRF
CCCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVEMVANELGVSPADVMEMESRMTGADV
CEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCE
AFDLPTDDNEETYSPSLYLEDKGSNFAAELENENYEAQATEQLSVALEGLDERSLDIIKA
EEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
RWLDENKATLQDLAAKYNISAERVRQLETNALKKLKSAVNF
HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKDTQTMMLVPQGSIEAYIRAANEYPMLTAEEEKELAERLYYQEDLEAAKKLILSHLRF
CCCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHVARGYSGYGLPQADLIQEGNIGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNENEVEMVANELGVSPADVMEMESRMTGADV
CEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCE
AFDLPTDDNEETYSPSLYLEDKGSNFAAELENENYEAQATEQLSVALEGLDERSLDIIKA
EEECCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
RWLDENKATLQDLAAKYNISAERVRQLETNALKKLKSAVNF
HHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]