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The map label for this gene is nudE [H]

Identifier: 15603409

GI number: 15603409

Start: 1747980

End: 1748531

Strand: Reverse

Name: nudE [H]

Synonym: PM1544

Alternate gene names: 15603409

Gene position: 1748531-1747980 (Counterclockwise)

Preceding gene: 15603423

Following gene: 15603408

Centisome position: 77.45

GC content: 42.21

Gene sequence:

>552_bases
ATGCATAAAAAACCGGAAATCCTCTCTGTGTCAGTGGTTGCCAAATCACGTATTTTTGAGATTCAATCGGTCGAACTCCA
ATTTTCGAATGGGATTCAGCGTACTTATGAACGCTTTAAAGGGACTGGACGTGCTGCGGTCATGGTGCTACCCATTGAAA
ATGACCAATTACTGATGGTGCGAGAATATGCGATGGGCACAGAGCAATACGAACTGGGTTTCCCTAAAGGTTTAATGGAT
CCCAATGAAACACCTGAAGAAAGTGCTAATCGAGAACTCAAAGAAGAAGTGGGATTAGGTGCTAACGAATTCATTCATTT
ACGCACAGTCAATACCTCGCCAAGTTACATGAATAGCGTGATGCATATCTTTTTAGCTCGGCACCTTTATCCCTGCAAAC
TGGAAGGCGACGAGCCTGAACCCCTAGAACAAATCAGATTCCCTCTCTGTCAATTAGACGACTTATTGCAAGATCCTACA
TTCCGTGAAGCACGTAATTTAGTTGCACTGTATGCACTACGTGACTTTTTAACAAAAAATAAACATCGTTAA

Upstream 100 bases:

>100_bases
TTCATGTCTATCATGCACATCACTTTCCTTTTGGTGTCATGCGGTTTATGGTATCGTTATTCCCTAATTTTGTAAAATTG
CACTTATCCACGAGGTGAAT

Downstream 100 bases:

>100_bases
AAAAATGAGTGGTCGATATCATCACCTCTCTCTCAGTTAAGCTGTCTTATTTTATAGATTCGCTTAATTTATATTAAAAA
AACGTATTGTTTTAAATCAA

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MHKKPEILSVSVVAKSRIFEIQSVELQFSNGIQRTYERFKGTGRAAVMVLPIENDQLLMVREYAMGTEQYELGFPKGLMD
PNETPEESANRELKEEVGLGANEFIHLRTVNTSPSYMNSVMHIFLARHLYPCKLEGDEPEPLEQIRFPLCQLDDLLQDPT
FREARNLVALYALRDFLTKNKHR

Sequences:

>Translated_183_residues
MHKKPEILSVSVVAKSRIFEIQSVELQFSNGIQRTYERFKGTGRAAVMVLPIENDQLLMVREYAMGTEQYELGFPKGLMD
PNETPEESANRELKEEVGLGANEFIHLRTVNTSPSYMNSVMHIFLARHLYPCKLEGDEPEPLEQIRFPLCQLDDLLQDPT
FREARNLVALYALRDFLTKNKHR
>Mature_183_residues
MHKKPEILSVSVVAKSRIFEIQSVELQFSNGIQRTYERFKGTGRAAVMVLPIENDQLLMVREYAMGTEQYELGFPKGLMD
PNETPEESANRELKEEVGLGANEFIHLRTVNTSPSYMNSVMHIFLARHLYPCKLEGDEPEPLEQIRFPLCQLDDLLQDPT
FREARNLVALYALRDFLTKNKHR

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=175, Percent_Identity=57.1428571428571, Blast_Score=220, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21127; Mature: 21127

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKPEILSVSVVAKSRIFEIQSVELQFSNGIQRTYERFKGTGRAAVMVLPIENDQLLMV
CCCCCCEEEEEEEHHHHHEEEEEEEEEHHCCHHHHHHHHCCCCCEEEEEEEECCCCEEEE
REYAMGTEQYELGFPKGLMDPNETPEESANRELKEEVGLGANEFIHLRTVNTSPSYMNSV
EHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHH
MHIFLARHLYPCKLEGDEPEPLEQIRFPLCQLDDLLQDPTFREARNLVALYALRDFLTKN
HHHHHHHHCCCEEECCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KHR
CCC
>Mature Secondary Structure
MHKKPEILSVSVVAKSRIFEIQSVELQFSNGIQRTYERFKGTGRAAVMVLPIENDQLLMV
CCCCCCEEEEEEEHHHHHEEEEEEEEEHHCCHHHHHHHHCCCCCEEEEEEEECCCCEEEE
REYAMGTEQYELGFPKGLMDPNETPEESANRELKEEVGLGANEFIHLRTVNTSPSYMNSV
EHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHH
MHIFLARHLYPCKLEGDEPEPLEQIRFPLCQLDDLLQDPTFREARNLVALYALRDFLTKN
HHHHHHHHCCCEEECCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KHR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]